1887

Abstract

The anthranilate-5-phosphoribosylpyrophosphate phosphoribosyltransferases (PRT), coded by the second structural gene () of the tryptophan () operon in strains LT2 and LT7 of , differ from each other in a number of parameters. These include the apparent values for their substrates anthranilic acid and 5-phosphoribosylpyro-phosphate, thermostability, sensitivity to substrate inhibition by anthranilic acid, as well as end-product inhibition by tryptophan and specific activity. The PRT of strain LT7 further differs from that of strain LT2 in that its apparent for 5-phosphoribosylpyrophosphate is three to seven times higher when associated with anthranilate synthase in the enzyme complex which catalyses the first two steps of tryptophan biosynthesis than in its free uncomplexed form, while the PRT of strain LT2 shows the same apparent for this substrate in both its free and complexed forms. These results confirm and extend the finding of Stuttard (1975) that strains LT2 and LT7 differ genetically from each other at a single site within region II of the gene.

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1979-05-01
2021-05-08
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References

  1. Balbinder E., Blume A., Weber A., Tamaki H. 1968; Polar and antipolar mutants in the tryptophan operon of Salmonella typhimurium. Journal of Bacteriology 95:2217–2229
    [Google Scholar]
  2. Balbinder E., Callahan R., Mccann P. P., Cordaro J. C., Weber A. R., Smith A. M., Angelosanto F. 1970; Regulatory mutants of the tryptophan operon of Salmonella typhimurium . Genetics 66:31–53
    [Google Scholar]
  3. Bauerle R. H., Margolin P. 1966; A multifunctional enzyme complex in the tryptophan pathway of Salmonella typhimurium comparison of polarity and pseudopolarity mutations. Cold Spring Harbor Symposia on Quantitative Biology 31:203–214
    [Google Scholar]
  4. Bauerle R. H., Margolin P. 1967; evidence for two sites for initiation of gene expression in the tryptophan operon of Salmonella typhimurium. journal of molecular biology 26:423–436
    [Google Scholar]
  5. Blume A. J., Balbinder E. 1966; the tryptophan operon of Salmonella typhimurium fine structure analysis by deletion mapping and abortive transduction. Genetics 53:577–592
    [Google Scholar]
  6. Blume A. J., Weber A., Balbinder E. 1968; Analysis of polar and nonpolar tryptophanmutants by derepression kinetics. Journal of Bacteriology 95:2230–2241
    [Google Scholar]
  7. Cordaro J. C., Levy H. R., Balbinder E. 1968; Product inhibition of anthranilate synthetase in Salmonella typhimurium. Biochemical and Biophysical Research Communications 33:183–189
    [Google Scholar]
  8. Creighton T. E. 1970; A steady-state kinetic investigation of the reaction mechanism of the tryptophan synthetase of escherichia coli. European Journal of Biochemistry 13:1–10
    [Google Scholar]
  9. Demerec M., Adelberg E. A., Clark A. J., Hartman P. E. 1966; A proposal for a uniform nomenclature in bacterial genetics. Genetics 54:61–76
    [Google Scholar]
  10. Gerhart J. C., Pardee A. B. 1962; The enzymology of control by feedback inhibition. Journal of Biological Chemistry 237:891–896
    [Google Scholar]
  11. Grieshaber M., Bauerle R. 1972; Structure and evolution of a bifunctional enzyme of the tryptophan operon. Nature New Biology 236:232–235
    [Google Scholar]
  12. Hardman J. K., Yanofsky C. 1965; Studies on the active site of the a protein subunit of the Escherichia coli tryptophan synthetase. Journal of Biological Chemistry 240:725–732
    [Google Scholar]
  13. Henderson E. J., Nagano H., Zalkin H., Hwang L. H. 1970a; the anthranilate synthetase- anthranilate 5-phosphoribosylpyrophosphate phosphoribosyltransferase aggregate. Purification of the aggregate and regulatory properties of anthranilate synthetase. Journal of Biological Chemistry 245:1416–1423
    [Google Scholar]
  14. Henderson E. J., Zalkin H., Hwang L. H. 1970b; The anthranilate synthetase-anthranilate 5-phosphoribosylpyrophosphate phosphoribosyl- transferase aggregate. catalytic and regulatory properties of aggregated and unaggregated forms of anthranilate 5-phosphoribosylpyrophosphate phosphoribosyltransferase. Journal of Biological Chemistry 245:1424–1431
    [Google Scholar]
  15. Jackson E. N., Yanofsky C. 1974; localization of two functions of phosphoribosyl anthranilate transferase of Escherichia coli to distinct regions of the polypeptide chain. Journal of Bacteriology 117:502–508
    [Google Scholar]
  16. La Scolea L. J., Balbinder E. 1972; Restoration of phosphoribosyltransferase activity by partially deleting the trpb gene in the tryptophan operon of Salmonella typhimurium. Journal of Bacteriology 112:877–885
    [Google Scholar]
  17. Lowry O. H., Rosebrough N. J., Farr A. L., Randall R. J. 1951; Protein measurement with the folin phenol reagent. Journal Of Biological Chemistry 193:265–275
    [Google Scholar]
  18. Nagano H., Zalkin H. 1970; Some physicochemical properties of anthranilate synthetase component I from Salmonella typhimurium. Journal of Biological Chemistry 245:3097–3103
    [Google Scholar]
  19. Pabst M. J., Kuhn J. C., Somerville R. L. 1973; Feedback regulation in the anthranilate aggregate from wild type and mutant strains of Escherichia coli . Journal of Biological Chemistry 248:901–914
    [Google Scholar]
  20. Robison P. D., Levy H. R. 1976; Metal ion requirement and tryptophan inhibition by normal and variant anthranilate synthetase-anthranilate- 5-phosphoribosylpyrophosphate phosphoribosyltransferases from Salmonella typhimurium. Biochimica et biophysica acta 445:475–485
    [Google Scholar]
  21. Smith O. H., Yanofsky C. 1962; Enzymes involved in the biosynthesis of tryptophan. Methods in enzymology 5:794–806
    [Google Scholar]
  22. Stuttard C. 1975; Tryptophan biosynthesis in Salmonella typhimurium: location in trpB of a genetic difference between strains LT2 and LT7. Journal of Bacteriology 123:878–887
    [Google Scholar]
  23. Vogel H. J., Bonner D. 1956; Acetylornithinase of escherichia coli: partial purification and some properties. Journal of Biological Chemistry 218:97–106
    [Google Scholar]
  24. Yanofsky C., Horn V., Bonner M., Stasiowski S. 1971; Polarity and enzyme functions in mutants for the first three genes of the tryptophan operon of Escherichia coli . Genetics 69:409–433
    [Google Scholar]
  25. Zalkin H. 1973; Anthranilate synthetase. Advances in Enzymology 38:1–39
    [Google Scholar]
  26. Zalkin H., Kling D. 1968; anthranilate synthetase - purification and properties of component I from Salmonella typhimurium . Biochemistry 7:3566–3573
    [Google Scholar]
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