1887

Abstract

SUMMARY: The effect of dissolved oxygen concentration on glucose metabolism, poly-β-hydroxybutyr-ate (PHB) synthesis and respiration of nitrogen-fixing was investigated over a range of values spanning oxygen limitation and sufficiency (nitrogen limitation) in continuous culture. The activities of the Entner-Doudoroff enzymes decreased with increasing oxygen supply while glucose-6-phosphate dehydrogenase was unaffected; β-ketothiolase and acetoacetyl-CoA reductase decreased more markedly, reflecting the fall in PHB content. The metabolic quotients for O and CO increased rapidly with increasing oxygen supply, in keeping with the respiratory protection of nitrogenase. The specific activities of all these enzymes, and also of fructose-1,6-bisphosphate aldolase, increased with increasing dilution rate under either oxygen or nitrogen limitation, except that both β-ketothiolase and acetoacetyl-CoA reductase activities fell at the highest dilution rate (0·23 h) under oxygen limitation. It was concluded that the role of the Entner-Doudoroff sequence as the major route of glucose metabolism in was unaffected by oxygen concentration and growth rate.

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/content/journal/micro/10.1099/00221287-110-2-393
1979-02-01
2021-07-30
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References

  1. Dalton H., Postgate J. R. 1968; Effect of oxygen on growth of Azotobacter chroococcum in batch and continuous cultures. Journal of General Microbiology 54:463–473
    [Google Scholar]
  2. Drozd J., Postgate J. R. 1970; Effects of oxygen on acetylene reduction, cytochrome content and respiratory activity of Azotobacter chroococcum. Journal of General Microbiology 63:63–73
    [Google Scholar]
  3. Haaker H., Veeger C. 1976; Regulation of respiration and nitrogen fixation in different types of Azotobacter vinelandii. European Journal of Biochemistry 63:499–507
    [Google Scholar]
  4. Jackson F. A., Dawes E. A. 1976; Regulation of the tricarboxylic acid cycle and poly-β-hydroxybutyrate metabolism in Azotobacter beijerinckii grown under nitrogen or oxygen limitation. Journal of General Microbiology 97:303–312
    [Google Scholar]
  5. Law J. H., Slepecky R. A. 1961; Assay of poly-β-hydroxybutyric acid. Journal of Bacteriology 82:33–36
    [Google Scholar]
  6. McInnes A. G., Ball D. H., Cooper F. P., Bishop C. T. 1958; Separation of carbohydrate derivatives by gas–liquid partition chromatography. Journal of Chromatography 1:556–557
    [Google Scholar]
  7. Mortenson L. E., Hamilton P. B., Wilson P. W. 1955; Dissimilation of 6-phosphogluconate by Azotobacter vinelandii. Biochimica et biophysica acta 16:238–244
    [Google Scholar]
  8. Nagai S., Nishizawa Y., Onodera M., Aiba S. 1971; Effect of dissolved oxygen on growth yield and aldolase activity in chemostat culture of Azotobacter vinelandii. Journal of General Microbiology 66:197–203
    [Google Scholar]
  9. Nagai S., Nishizawa Y., Aiba S. 1974; Some consideration on the rate of induced aldolase synthesis in Azotobacter vinelandii. Journal of General and Applied Microbiology 20:229–241
    [Google Scholar]
  10. Parker C. A. 1954; Effect of oxygen on nitrogen fixation by Azotobacter. Nature, London 173:780–781
    [Google Scholar]
  11. Phillips D. A., Johnson M. J. 1961; Aeration in fermentations. Journal of Biochemical and Microbiological Technology and Engineering 3:277–309
    [Google Scholar]
  12. Senior P. J., Dawes E. A. 1971; Poly-β-hydroxybutyrate biosynthesis and the regulation of glucose metabolism in Azotobacter beijerinckii. Biochemical Journal 125:55–66
    [Google Scholar]
  13. Senior P. J., Dawes E. A. 1973; The regulation of poly-β-hydroxybutyrate metabolism in Azotobacter beijerinckii. Biochemical Journal 134:225–238
    [Google Scholar]
  14. Senior P. J., Beech G. A., Ritchie G. A. F., Dawes E. A. 1972; The role of oxygen limitation in the formation of poly-β-hydroxybutyrate during batch and continuous culture of Azotobacter beijerinckii. Biochemical Journal 128:1193–1201
    [Google Scholar]
  15. Stephenson M. P., Jackson F. A., Dawes E. A. 1978; Further observations on carbohydrate metabolism and its regulation in Azotobacter beijerinckii. Journal of General Microbiology 109:89–96
    [Google Scholar]
  16. Ward A. C., Rowley B. I., Dawes E. A. 1977; Effect of oxygen and nitrogen limitation on poly-β-hydroxybutyrate biosynthesis in ammonium-grown Azotobacter beijerinckii. Journal of General Microbiology 102:61–68
    [Google Scholar]
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