Most of the hybrids from crosses between the donor Hfr45(O8:K27) and different O9 recipients expressed the donor O8 antigen specificity and produced the capsular antigen K27. Therefore these hybrids must have inherited the -linked donor region determining the synthesis of O8-specific polysaccharides as well as -linked genes involved in K27 antigen synthesis. In the living state these hybrids were inagglutinable in O8 antiserum like the donor cells. However, when 12 and O8:K42 were used as recipients most of the hybrids were agglutinable in O8 and K27 antisera. The amounts of K27 antigen present in these hybrids, designated as K27i (intermediate) forms, were sufficient to evoke the production of K27 antibodies in rabbits, but insufficient to inhibit O-agglutination of the respective cells. The additional transfer of the region of O8:K27 into such K27i forms frequently resulted in O-inagglutinable K27 hybrids. This is attributed to the introduction of -linked genes which apparently play a role in the synthesis of K27 capsular antigen. Thus it is concluded that at least two gene loci, one close to and the other close to , are required for the synthesis of the complete capsular antigen K27.


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