1887

Abstract

SUMMARY: A dilution method for the assay of streptomycin is described using a digest nutrient broth as the test medium and a certain strain of ( as the test organism. The inhibition end-point is estimated turbidi-metrically by comparison with that in a standard solution of streptomycin base. The accuracy of the method when employed as described is of the order of ± 15%. It is necessary to control the dilution of the test medium, the temperature of incubation of the tests, the size of the inoculum and hydrogen-ion concentration in order to obtain consistent results. With dilution of the medium the sensitivity of the test is increased while its accuracy is decreased. The use of an inoculum of constant size is important, and an incubation temperature of 28° is found to be more satisfactory than 37°. Medium with a hydrogen-ion concentration of pH 7·6 affords greater sensitivity than that with a lower pH value.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-1-3-353
1947-09-01
2021-08-02
Loading full text...

Full text loading...

/deliver/fulltext/micro/1/3/mic-1-3-353.html?itemId=/content/journal/micro/10.1099/00221287-1-3-353&mimeType=html&fmt=ahah

References

  1. Abraham E. P., Duthie E. S. 1946; Effect of pH of the medium on activity of streptomycin and penicillin. Lancet i:455
    [Google Scholar]
  2. Ainsworth G. C., Brown A. M., Marsden P. S. S. F., Smith P. A., Spilsbury J. F. 1947; A method for the large-scale production of streptomycin by surface culture. J. gen. Microbiol 1:335
    [Google Scholar]
  3. Bondi A., Dietz C. C., Spaulding E. H. 1946; Interference with the antibacterial action of streptomycin by reducing agents. Science 103:399
    [Google Scholar]
  4. Cavallito C. J. 1946; Relationship of thiol structures to reaction with antibiotics. J. biol. chem 164:29
    [Google Scholar]
  5. Denkelwater R., Cook M. A., Tisiiler M. 1945; The effect of cysteine on streptomycin & streptothricin. Science 102:12
    [Google Scholar]
  6. Donovick R., Rake G. 1946; Influence of certain substances on activity of streptomycin in vitro. 1. Modifications in test medium. Proc. Soc. exp. Biol., N.Y 61:224
    [Google Scholar]
  7. Donovick R., Rake G., Fried J. 1946; The influence of certain substances on the antibiotic activity of streptomycin in vitro. 2. The action of some carbonyl reagents on streptomycin. J. biol. Chem 164:173
    [Google Scholar]
  8. Geiger W. B., Green S. R., Waksman S. A. 1946; The inactivation of streptomycin and its practical applications. Proc. Soc. exp. Biol., N.Y 61:187–92
    [Google Scholar]
  9. Hobby G. L., Lenert F., Hyman B. 1946; Factors influencing the action of streptomycin in vitro. J. Bact 51:606
    [Google Scholar]
  10. Madigan D. G., Swift P. N., Brownlee G. 1947; Clinical & pharmacological aspects of the toxicity of streptomycin. Lancet i:9
    [Google Scholar]
  11. Molitor H., Graessle O. E., Kuna S., Mushett C. W., Silber R. H. 1946; Some toxiological and pharmacological properties of streptomycin. J. Pharmacol 86:151
    [Google Scholar]
  12. Pope C. G., Stevens M. F. 1946; The assay of penicillin by the dilution method. Bull. Hlth Org. L.o.N 12:274
    [Google Scholar]
  13. Van Dolah R. W., Christenson G. L. 1947; Chemical inactivation of streptomycin. Arch. Biochem 12:7
    [Google Scholar]
  14. Waksman S. 1945; Standardization of streptomycin. Science 102:40
    [Google Scholar]
  15. Waksman S., Schatz A. 1945; Streptomycin-origin, nature and properties. J. Amer. pharm. Ass 34:273
    [Google Scholar]
  16. Wolinsky E., Steenken W. 1946; Streptomycin and penicillin resistant staphylococci; influence of pH, and body fluids on streptomycin action. Proc. Soc. exp. Biol., N.Y 62:162
    [Google Scholar]
  17. Woodthorpe T. J., Ireland D. M. 1947; A method for extracting and purifying streptomycin suitable for large-scale production. J. gen. Microbiol 1:334
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-1-3-353
Loading
/content/journal/micro/10.1099/00221287-1-3-353
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error