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Volume 10, Issue 3

Virulence genes, resistome and mobilome of strains isolated in France Open Access

Abstract

is a leading cause of infection in pigs, causing extensive economic losses. In addition, it can also infect wild fauna, and can be responsible for severe infections in humans. Increasing antimicrobial resistance (AMR) has been described in worldwide and most of the AMR genes are carried by mobile genetic elements (MGEs). This contributes to their dissemination by horizontal gene transfer. A collection of 102 strains isolated from humans, pigs and wild boars in France was subjected to whole genome sequencing in order to: (i) study their genetic diversity, (ii) evaluate their content in virulence-associated genes, (iii) decipher the mechanisms responsible for their AMR and their association with MGEs, and (iv) study their ability to acquire extracellular DNA by natural transformation. Analysis by hierarchical clustering on principal components identified a few virulence-associated factors that distinguish invasive CC1 strains from the other strains. A plethora of AMR genes (=217) was found in the genomes. Apart from the frequently reported (B) and (O) genes, more recently described AMR genes were identified [(F)/, (D)]. Modifications in PBPs/MraY and GyrA/ParC were detected in the penicillin- and fluoroquinolone-resistant isolates respectively. New AMR gene–MGE associations were detected. The majority of the strains have the full set of genes required for competence, i.e for the acquisition of extracellular DNA (that could carry AMR genes) by natural transformation. Hence the risk of dissemination of these AMR genes should not be neglected.

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Keyword(s): competence , integrative conjugative elements , mobilizable elements , resistance genes , Streptococcus suis and virulence
Funding
This study was supported by the:
  • Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement (INRAE)
    • Principle Award Recipient: ManonDechêne-Tempier
  • Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (Anses)
    • Principle Award Recipient: ManonDechêne-Tempier
© 2024 The Authors
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License. This article was made open access via a Publish and Read agreement between the Microbiology Society and the corresponding author’s institution.
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2024-04-27
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Virulence genes, resistome and mobilome of Streptococcus suis strains isolated in France
M Gen 10, 001224 (2024); https://doi.org/10.1099/mgen.0.001224
/content/journal/mgen/10.1099/mgen.0.001224
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