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Volume 10, Issue 3

Identifying the suite of genes central to swimming in the biocontrol bacterium Pf-5 Open Access

Abstract

Swimming motility is a key bacterial trait, important to success in many niches. Biocontrol bacteria, such as Pf-5, are increasingly used in agriculture to control crop diseases, where motility is important for colonization of the plant rhizosphere. Swimming motility typically involves a suite of flagella and chemotaxis genes, but the specific gene set employed for both regulation and biogenesis can differ substantially between organisms. Here we used transposon-directed insertion site sequencing (TraDIS), a genome-wide approach, to identify 249 genes involved in Pf-5 swimming motility. In addition to the expected flagella and chemotaxis, we also identified a suite of additional genes important for swimming, including genes related to peptidoglycan turnover, O-antigen biosynthesis, cell division, signal transduction, c-di-GMP turnover and phosphate transport, and 27 conserved hypothetical proteins. Gene knockout mutants and TraDIS data suggest that defects in the Pst phosphate transport system lead to enhanced swimming motility. Overall, this study expands our knowledge of pseudomonad motility and highlights the utility of a TraDIS-based approach for analysing the functions of thousands of genes. This work sets a foundation for understanding how swimming motility may be related to the inconsistency in biocontrol bacteria performance in the field.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): biocontrol , motility , plant growth-promoting rhizobacteria , swimming , TraDIS and transposon insertion sequencing
Funding
This study was supported by the:
  • Australian Research Council (Award FT180100123)
    • Principle Award Recipient: KA Hassan
  • Australian Research Council (Award DP160103746)
    • Principle Award Recipient: SashaG Tetu
© 2024 The Authors
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License. This article was made open access via a Publish and Read agreement between the Microbiology Society and the corresponding author’s institution.
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2024-04-28
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Identifying the suite of genes central to swimming in the biocontrol bacterium Pseudomonas protegens Pf-5
M Gen 10, 001212 (2024); https://doi.org/10.1099/mgen.0.001212
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