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Volume 10, Issue 3

Genomic analysis of clinical isolates reveals genetic diversity but little evidence of genetic determinants for diarrhoeal disease Open Access

Abstract

spp. are associated with a number of infectious syndromes in humans including gastroenteritis and dysentery. Our understanding of the genetic diversity, population structure, virulence determinants and antimicrobial resistance of the genus has been limited by a lack of sequenced genomes linked to metadata. We performed a comprehensive analysis of the whole genome sequences of 447 isolates from children in Karachi, Pakistan, with moderate-to-severe diarrhoea (MSD) and from matched controls without diarrhoea that were collected as part of the Global Enteric Multicenter Study (GEMS). Human-associated isolates exhibited high species diversity and extensive antimicrobial and virulence gene content. , , and were all significantly associated with MSD in at least one cohort group. The and genes that encode components of polar and lateral flagella, respectively, exhibited a weak association with isolates originating from cases of gastroenteritis.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Aeromonas , AMR , diarrhoea , GEMS , genomics , Pakistan and virulence
Funding
This study was supported by the:
  • Biotechnology and Biological Sciences Research Council (Award BB/X011011/1)
    • Principle Award Recipient: RobertA Kingsley
  • Biotechnology and Biological Sciences Research Council (Award BB/R012504/1)
    • Principle Award Recipient: RobertA Kingsley
© 2024 The Authors
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License. This article was made open access via a Publish and Read agreement between the Microbiology Society and the corresponding author’s institution.
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2024-03-07
2024-04-27
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Genomic analysis of clinical Aeromonas isolates reveals genetic diversity but little evidence of genetic determinants for diarrhoeal disease
M Gen 10, 001211 (2024); https://doi.org/10.1099/mgen.0.001211
/content/journal/mgen/10.1099/mgen.0.001211
/content/journal/mgen/10.1099/mgen.0.001211
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