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Volume 9, Issue 11

Comparative analysis of mitochondrion-related organelles in anaerobic amoebozoans Open Access

Abstract

Archamoebae comprises free-living or endobiotic amoebiform protists that inhabit anaerobic or microaerophilic environments and possess mitochondrion-related organelles (MROs) adapted to function anaerobically. We compared reconstructed MRO proteomes of eight species (six genera) and found that the common ancestor of Archamoebae possessed very few typical components of the protein translocation machinery, electron transport chain and tricarboxylic acid cycle. On the other hand, it contained a sulphate activation pathway and bacterial iron–sulphur (Fe-S) assembly system of MIS-type. The metabolic capacity of the MROs, however, varies markedly within this clade. The glycine cleavage system is widely conserved among Archamoebae, except in , probably owing to its role in catabolic function or one-carbon metabolism. MRO-based pyruvate metabolism was dispensed within subgroups Entamoebidae and Rhizomastixidae, whereas sulphate activation could have been lost in isolated cases of , and sp. The MIS (Fe-S) assembly system was duplicated in the common ancestor of Mastigamoebidae and Pelomyxidae, and one of the copies took over Fe-S assembly in their MRO. In Entamoebidae and Rhizomastixidae, we hypothesize that Fe-S cluster assembly in both compartments may be facilitated by dual localization of the single system. We could not find evidence for changes in metabolic functions of the MRO in response to changes in habitat; it appears that such environmental drivers do not strongly affect MRO reduction in this group of eukaryotes.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): anaerobiosis , comparative genomics , mitochondrion-related organelles and reductive evolution
Funding
This study was supported by the:
  • Ministerstvo Školství, Mládeže a Tělovýchovy (Award 90254)
    • Principle Award Recipient: NotApplicable
  • Canadian Institutes of Health Research (Award FRN-142349)
    • Principle Award Recipient: AndrewJ. Roger
  • Grantová Agentura České Republiky (Award 23-06004S)
    • Principle Award Recipient: IvanČepička
  • Ministerstvo Školství, Mládeže a Tělovýchovy (Award CZ.02.1.01/0.0/0.0/16_019/0000759)
    • Principle Award Recipient: JanTachezy
  • H2020 European Research Council (Award 771592)
    • Principle Award Recipient: VladimírHampl
© 2023 The Authors
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License.
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2023-11-23
2024-04-29
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http://instance.metastore.ingenta.com/content/journal/mgen/10.1099/mgen.0.001143
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Comparative analysis of mitochondrion-related organelles in anaerobic amoebozoans
M Gen 9, 001143 (2023); https://doi.org/10.1099/mgen.0.001143
/content/journal/mgen/10.1099/mgen.0.001143
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