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Volume 7, Issue 12

Single-cell genomics-based analysis reveals a vital ecological role of sp. LSW in the meromictic Lake Shunet, Siberia Open Access

Abstract

Meromictic lakes usually harbour certain prevailing anoxygenic phototrophic bacteria in their anoxic zone, such as the purple sulfur bacterium (PSB) sp. LSW (hereafter LSW) in Lake Shunet, Siberia. PSBs have been suggested to play a vital role in carbon, nitrogen and sulfur cycling at the oxic–anoxic interface of stratified lakes; however, the ecological significance of PSBs in the lake remains poorly understood. In this study, we explored the potential ecological role of LSW using a deep-sequencing analysis of single-cell genomics associated with flow cytometry. An approximately 2.7 Mb draft genome was obtained based on the co-assembly of five single-cell genomes. LSW might grow photolithoautotrophically and could play putative roles not only as a carbon fixer and diazotroph, but also as a sulfate reducer/oxidizer in the lake. This study provides insights into the potential ecological role of sp. in meromictic lakes.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): flow cytometry , Lake Shunet , purple sulfur bacteria and single-cell genomics
Funding
This study was supported by the:
  • Ministry of Science and Technology, Taiwan (Award MOST105-2923-B-001-001-MY3)
    • Principle Award Recipient: Sen-LinTang
  • Russian Foundation for Basic Research (Award 21-54-52001)
    • Principle Award Recipient: DenisYu Rogozin
  • Ministry of Science and Technology, Taiwan (Award NSC102-2923-B-001-004-MY3)
    • Principle Award Recipient: Sen-LinTang
© 2021 The Authors
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License.
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2021-12-03
2024-04-26
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Single-cell genomics-based analysis reveals a vital ecological role of Thiocapsa sp. LSW in the meromictic Lake Shunet, Siberia
M Gen 7, 000712 (2021); https://doi.org/10.1099/mgen.0.000712
/content/journal/mgen/10.1099/mgen.0.000712
/content/journal/mgen/10.1099/mgen.0.000712
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