1887

Abstract

Increasing antimicrobial resistance and limited alternative treatments have led to fluoroquinolone-resistant strain inclusion on the WHO global priority pathogens list. In this study we characterized multiple isolates from Malawi with whole genome sequence analysis, identifying the acquirable fluoroquinolone resistance determinant .

Keyword(s): AMR , FQR , Shigella and WGS
Funding
This study was supported by the:
  • Wellcome Trust (Award 098051)
    • Principle Award Recipient: NotApplicable
  • Centre of Excellence in Cognition and its Disorders, Australian Research Council (Award DE180100929)
    • Principle Award Recipient: AmyCain
  • Medical Research Council (Award MR/R020787/1)
    • Principle Award Recipient: KateS. Baker
  • Wellcome Trust (Award 106690/A/14/Z)
    • Principle Award Recipient: KateS. Baker
  • Medical Research Council (Award MR/N013840/1)
    • Principle Award Recipient: NotApplicable
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2021-05-04
2021-05-15
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References

  1. Khalil IA, Troeger C, Blacker BF, Rao PC, Brown A. Morbidity and mortality due to Shigella and enterotoxigenic Escherichia coli diarrhoea: the global burden of disease study 1990–2016. Lancet Infectious Disease 2018; 2018:1229–1240
    [Google Scholar]
  2. Troeger C, Forouzanfar M, Rao PC, lI K, Brown AE et al. Estimates of global, regional, and national morbidity, mortality, and aetiologies of diarrhoeal diseases: a systematic analysis for the global burden of disease study 2015. Lancet Infect Dis 2017; 17:909–948 [CrossRef][PubMed]
    [Google Scholar]
  3. Gu B, Cao Y, Pan S, Zhuang L, Yu R et al. Comparison of the prevalence and changing resistance to nalidixic acid and ciprofloxacin of Shigella between Europe-America and Asia-Africa from 1998 to 2009. Int J Antimicrob Agents 2012; 40:9–17 [CrossRef][PubMed]
    [Google Scholar]
  4. WHO Global priority list of antibiotic-resistant bacteria to guide research, discovery, and development of new antibiotics. WHO press 2017; 7:
    [Google Scholar]
  5. Nüesch-Inderbinen M, Heini N, Zurfluh K, Althaus D, Hächler H et al. Shigella antimicrobial drug resistance mechanisms, 2004-2014. Emerg Infect Dis 2016; 22:1083–1085 [CrossRef][PubMed]
    [Google Scholar]
  6. Iturriza-Gomara M, Jere KC, Hungerford D, Bar-Zeev N, Shioda K et al. Etiology of diarrhea among hospitalized children in Blantyre, Malawi, following rotavirus vaccine introduction: a case-control study. J Infect Dis 2019; 220:213–218 [CrossRef][PubMed]
    [Google Scholar]
  7. Baker KS, Campos J, Pichel M, Della Gaspera A, Duarte-Martinez F et al. Whole genome sequencing of Shigella sonnei through PulseNet Latin America and Caribbean: advancing global surveillance of foodborne illnesses. Clin Microbiol Infect 2017; 23:845–853 [CrossRef][PubMed]
    [Google Scholar]
  8. WHO Draft who preferred product characteristics for vaccines against Shigella . WHO press 2020
    [Google Scholar]
  9. Bar-Zeev N, Kapanda L, Tate JE, Jere KC, Iturriza-Gomara M et al. Effectiveness of a monovalent rotavirus vaccine in infants in Malawi after programmatic roll-out: an observational and case-control study. Lancet Infect Dis 2015; 15:422–428 [CrossRef][PubMed]
    [Google Scholar]
  10. Kotloff KL, Riddle MS, Platts-Mills JA, Pavlinac P, Zaidi AKM. Shigellosis. The Lancet 2018; 391:801–812 [CrossRef]
    [Google Scholar]
  11. Livio S, Strockbine NA, Panchalingam S, Tennant SM, Barry EM et al. Shigella isolates from the global enteric multicenter study inform vaccine development. Clin Infect Dis 2014; 59:933–941 [CrossRef][PubMed]
    [Google Scholar]
  12. Lan R, Lumb B, Ryan D, Reeves PR. Molecular evolution of large virulence plasmid in Shigella clones and enteroinvasive Escherichia coli . Infect Immun 2001; 69:6303–6309 [CrossRef][PubMed]
    [Google Scholar]
  13. Schroeder GN, Hilbi H. Molecular pathogenesis of Shigella spp.: controlling host cell signaling, invasion, and death by type III secretion. Clin Microbiol Rev 2008; 21:134–156 [CrossRef][PubMed]
    [Google Scholar]
  14. Wick RR, Judd LM, Gorrie CL, Holt KE. Unicycler: resolving bacterial genome assemblies from short and long sequencing reads. PLoS Comput Biol 2017; 13:e1005595 [CrossRef][PubMed]
    [Google Scholar]
  15. Baker KS, Dallman TJ, Field N, Childs T, Mitchell H et al. Horizontal antimicrobial resistance transfer drives epidemics of multiple Shigella species. Nat Commun 2018; 9:1462 [CrossRef][PubMed]
    [Google Scholar]
  16. Ingle DJ, Easton M, Valcanis M, Seemann T, Kwong JC et al. Co-circulation of multidrug-resistant Shigella among men who have sex with men in Australia. Clin Infect Dis 2019; 69:1535–1544 [CrossRef][PubMed]
    [Google Scholar]
  17. Liang B, Roberts AP, Xu X, Yang C, Yang X et al. Transferable Plasmid-Borne mcr-1 in a Colistin-Resistant Shigella flexneri isolate. Appl Environ Microbiol 2018; 84:e02655–17 [CrossRef][PubMed]
    [Google Scholar]
  18. Schroeder MR, Juieng P, Batra D, Knipe K, Rowe LA et al. High-Quality complete and draft genome sequences for three Escherichia spp. and three Shigella spp. generated with Pacific biosciences and illumina sequencing and optical. Mapping Genome Announcements 2018; Ja;6:e01384–17
    [Google Scholar]
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