1887

Abstract

chronic airway infection in patients with cystic fibrosis (CF) allows this pathogen to adapt over time in response to different selection pressures. We have previously shown that the main sequence types related to community-acquired methicillin-resistant (MRSA) infections in Argentina – ST5 and ST30 – are also frequently isolated from the sputum of patients with CF, but in these patients they usually display multi-drug antimicrobial resistance. In this study, we sequenced the genomes of MRSA from four paediatric CF patients with the goal of identifying mutations among sequential isolates, especially those possibly related to antimicrobial resistance and virulence, which might contribute to the adaptation of the pathogen in the airways of patients with CF. Our results revealed genetic differences in sequential MRSA strains isolated from patients with CF in both their core and accessory genomes. Although the genetic adaptation of was distinct in different hosts, we detected independent mutations in and – which are known to have crucial roles in virulence and antimicrobial resistance – in isolates recovered from multiple patients. Moreover, we identified allelic variants that were detected in all of the isolates recovered after a certain time point; these non-synonymous mutations were in genes associated with antimicrobial resistance, virulence, iron scavenging and oxidative stress resistance. In conclusion, our results provide evidence of genetic variability among sequential MRSA isolates that could be implicated in the adaptation of these strains during chronic CF airway infection.

Keyword(s): cystic fibrosis , microevolution , MRSA , ST30 and ST5
Funding
This study was supported by the:
  • Consejo Nacional de Investigaciones Científicas y Técnicas (Award PIP 11220150100694CO)
    • Principle Award Recipient: MartaMollerach
  • Agencia Nacional de Promoción Científica y Tecnológica (Award PICT 2012 2362)
    • Principle Award Recipient: MartaMollerach
  • Secretaria de Ciencia y Tecnica, Universidad de Buenos Aires (Award UBACYT 20020130100381BA)
    • Principle Award Recipient: MartaMollerach
  • This is an open-access article distributed under the terms of the Creative Commons Attribution NonCommercial License.
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2021-02-18
2021-10-25
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