1887

Microbial Genomics logo

Volume 6, Issue 7

Comparative genomics of the genus reveals divergent biosynthetic pathways that may influence colonic competition among species Open Access

Abstract

species are important denizens of the human gut microbiome that ferment complex polysaccharides to butyrate as a terminal fermentation product, which influences human physiology and serves as an energy source for colonocytes. Previous comparative genomics analyses of the genus have examined polysaccharide degradation genes. Here, we characterize the core and pangenomes of the genus with respect to central carbon and energy metabolism, as well as biosynthesis of amino acids and B vitamins using orthology-based methods, uncovering significant differences among species in their biosynthetic capacities. Variation in gene content among species and strains was most significant for cofactor biosynthesis. Unlike all other species of that we analysed, strains lacked biosynthetic genes for riboflavin or pantothenate but possessed folate biosynthesis genes. Differences in gene content for B vitamin synthesis were matched with differences in putative salvage and synthesis strategies among species. For example, we observed extended biotin salvage capabilities in strains, which further suggest that B vitamin acquisition strategies may impact fitness in the gut ecosystem. As differences in the functional potential to synthesize components of biomass (e.g. amino acids, vitamins) can drive interspecies interactions, variation in auxotrophies of the spp. genomes may influence gut ecology. This study serves to advance our understanding of the potential metabolic interactions that influence the ecology of spp. and, ultimately, may provide a basis for rational strategies to manipulate the abundances of these species.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): amino acid biosynthesis , B vitamin biosynthesis , butyrate synthesis , comparative genomics , Lachnospiraceae and Roseburia
© 2020 The Authors
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License.
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2020-06-26
2024-04-26
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Comparative genomics of the genus Roseburia reveals divergent biosynthetic pathways that may influence colonic competition among species
M Gen 6, e000399 (2020); https://doi.org/10.1099/mgen.0.000399
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