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Volume 6, Issue 1

Large-scale and significant expression from pseudogenes in – a facultative bacterial endosymbiont Open Access

Abstract

The majority of bacterial genomes have high coding efficiencies, but there are some genomes of intracellular bacteria that have low gene density. The genome of the endosymbiont contains almost 50 % pseudogenes containing mutations that putatively silence them at the genomic level. We have applied multiple ‘omic’ strategies, combining Illumina and Pacific Biosciences Single-Molecule Real-Time DNA sequencing and annotation, stranded RNA sequencing and proteome analysis to better understand the transcriptional and translational landscape of pseudogenes, and potential mechanisms for their control. Between 53 and 74 % of the transcriptome remains active in cell-free culture. The mean sense transcription from coding domain sequences (CDSs) is four times greater than that from pseudogenes. Comparative genomic analysis of six Illumina-sequenced isolates from different host species shows pseudogenes make up ~40 % of the 2729 genes in the core genome, suggesting that they are stable and/or that is a recent introduction across the genus as a facultative symbiont. These data shed further light on the importance of transcriptional and translational control in deciphering host–microbe interactions. The combination of genomics, transcriptomics and proteomics gives a multidimensional perspective for studying prokaryotic genomes with a view to elucidating evolutionary adaptation to novel environmental niches.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): endosymbiont , pseudogenes , Sodalis glossinidius and transcriptome
Funding
This study was supported by the:
  • Wellcome Trust (Award 200690/Z/16/Z)
    • Principle Award Recipient: Ian Goodhead
  • Maihidol University (Award Studentship)
    • Principle Award Recipient: Pisut Pongchaikul
  • Biotechnology and Biological Sciences Research Council (Award BBJ017698/1)
    • Principle Award Recipient: Alistair C Darby
  • Biotechnology and Biological Sciences Research Council (Award BB/L014777/1)
    • Principle Award Recipient: Alistair C Darby
© 2020 The Authors
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License.
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2020-01-10
2024-04-26
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Large-scale and significant expression from pseudogenes in Sodalis glossinidius – a facultative bacterial endosymbiont
M Gen 6, e000285 (2020); https://doi.org/10.1099/mgen.0.000285
/content/journal/mgen/10.1099/mgen.0.000285
/content/journal/mgen/10.1099/mgen.0.000285
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