1887

Abstract

Introduction:

Infections due to are cumbersome to treat and control. Virulent is commonly coagulase positive, whilst non‐virulent isolates are coagulase negative. Literature on coagulase‐negative variants of and their role in clinical disease in neonates is seldom available.

Case presentation:

We report a case of an extremely low‐birth‐weight pre‐term infant who had persistent recalcitrant infection with coagulase‐negative methicillin‐sensitive , which resulted in clinical sepsis, thrombocytopaenia and persistence in blood cultures for 7 weeks. The API STAPH‐Ident system (Bio‐Merieux) and 16S rRNA gene sequencing were used to confirm identity. Parallel use of blood culture yielded an isolate with the same phenotypic identity.

Conclusion:

Prompt identification of uncommon phenotypes of also requires the use of appropriate molecular diagnostics to necessitate timely treatment of life‐threatening systemic infections in neonates.

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2014-09-01
2019-10-23
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References

  1. Aswani V.H. , Shukla S.K. . ( 2011; ). Prevalence of Staphylococcus aureus and lack of its lytic bacteriophages in the anterior nares of patients and healthcare workers at a rural clinic. . Clin Med Res 9:, 75–81.[CrossRef]
    [Google Scholar]
  2. Bannerman T.L. , Peacock S.J. . ( 2007; ). Staphylococcus, Micrococcus and other catalase‐positive cocci. . In Manual of Clinical Microbiology, 9th edn, Vol. 1, pp. 390–441. Washington, DC:: ASM Press;.
    [Google Scholar]
  3. Craft A. , Finer N. . ( 2001; ). Nosocomial coagulase negative staphylococcal (CoNS) catheter‐related sepsis in preterm infants: definition, diagnosis, prophylaxis, and prevention. . J Perinatol 21:, 186–192.[CrossRef]
    [Google Scholar]
  4. Fung J.C. , Kaplan M.H. , Hsieh H.C. , Stephens A. , Tyburski M.B. , Tenenbaum M.J. . ( 1984; ). Two coagulase‐variant forms of Staphylococcus aureus isolated from blood cultures. . J Clin Microbiol 20:, 115–117.
    [Google Scholar]
  5. Haimi‐Cohen Y. , Vellozzi E.M. , Rubin L.G. . ( 2002; ). Initial concentration of Staphylococcus epidermidis in simulated pediatric blood cultures correlates with time to positive results with the automated, continuously monitored BACTEC blood culture system. . J Clin Microbiol 40:, 898–901.[CrossRef]
    [Google Scholar]
  6. Hira V. , Sluijter M. , Goessens W.H. , Ott A. , de Groot R. , Hermans P.W. , Kornelisse R.F. . ( 2010; ). Coagulase‐negative staphylococcal skin carriage among neonatal intensive care unit personnel: from population to infection. . J Clin Microbiol 48:, 3876–3881.[CrossRef]
    [Google Scholar]
  7. Jacquot A. , Neveu D. , Aujoulat F. , Mercier G. , Marchandin H. , Jumas‐Bilak E. , Picaud J.C. . ( 2011; ). Dynamics and clinical evolution of bacterial gut microflora in extremely premature patients. . J Pediatr 158:, 390–396.[CrossRef]
    [Google Scholar]
  8. Kloos W.E. , Wolfshohl J.F. . ( 1982; ). Identification of Staphylococcus species with the API STAPH‐IDENT System. . J Clin Microbiol 16:, 509–516.
    [Google Scholar]
  9. Młynarczyk G. , Kochman M. , Lawrynowicz M. , Fordymacki P. , Młynarczyk A. , Jeljaszewicz J. . ( 1998; ). Coagulase‐negative variants of methicillin‐resistant Staphylococcus aureus subsp. . aureus strains isolated from hospital specimens. Zentralbl Bakteriol 288:, 373–381.[CrossRef]
    [Google Scholar]
  10. Palis J. , Segel G.B. . ( 2010; ). Hematology of the fetus and newborn. . In Williams Hematology, , 8th. edn, pp. 87–104. Lichtman M. A. , Kipps T. J. , Seligsohn U. , Kaushansky K. , Prchal J. T. . Rochester, NY:: McGraw‐Hill;.
    [Google Scholar]
  11. Que Y.A. , Moreillon P. . ( 2010; ). Staphylococcus aureus (including staphylococcal toxic shock). . In Mandell, Douglas and Bennett’s Principle and Practice of Infectious Disease, 7th edn, pp. 2543–2578. Philadelphia, PA:: Churchill Livingstone/Elsevier;.
    [Google Scholar]
  12. Romano‐Bertrand S. , Filleron A. , Mesnage R. , Lotthé A. , Didelot M.N. , Burgel L. , Bilak E.J. , Cambonie G. , Parer S. . ( 2014; ). Staphylococcus aureus in a neonatal care center: methicillin‐susceptible strains should be a main concern. . Antimicrob Resist Infect Control 3:, 21.[CrossRef]
    [Google Scholar]
  13. Tan T.Y. , Ng S.Y. , He J. . ( 2008; ). Microbiological characteristics, presumptive identification, and antibiotic susceptibilities of Staphylococcus lugdunensis . . J Clin Microbiol 46:, 2393–2395.[CrossRef]
    [Google Scholar]
  14. Vandenesch F. , Bes M. , Lebeau C. , Greenland T. , Brun Y. , Etienne J. . ( 1993; ). Coagulase‐negative Staphylococcus aureus . . Lancet 342:, 995–996.[CrossRef]
    [Google Scholar]
  15. van der Mee‐Marquet N. , Epinette C. , Loyau J. , Arnault L. , Domelier A.S. , Losfelt B. , Girard N. , Quentin R. . Bloodstream Infection Study Group of the Relais d’Hygiène du Centre ( 2007; ). Staphylococcus aureus strains isolated from bloodstream infections changed significantly in 2006. . J Clin Microbiol 45:, 851–857.[CrossRef]
    [Google Scholar]
  16. van der Mee‐Marquet N. , François P. , Domelier A.S. , Arnault L. , Girard N. , Schrenzel J. , Quentin R. . Bloodstream Infection Study Group of the Réseau des Hygiénistes du Centre ( 2009; ). Variable‐number tandem repeat analysis and multilocus sequence typing data confirm the epidemiological changes observed with Staphylococcus aureus strains isolated from bloodstream infections. . J Clin Microbiol 47:, 2863–2871.[CrossRef]
    [Google Scholar]
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