1887

Abstract

Introduction:

The Gram-stained morphology of organisms from positive blood culture bottles often suggests diagnoses and therapeutic options. aggregate formation by the organism in this case led to initial confusion in the diagnosis.

Case presentation:

A 57-year-old woman with a recent stroke returned to the emergency department with acute exacerbation of her neurological deficit. Although afebrile, she had an elevated white blood cell count. Urine analysis revealed 4+ blood, nitrates, leukocyte esterase and bacteria. Blood and urine cultures were collected and intravenous piperacillin/tazobactam treatment was begun. The urine culture grew >100 000 colonies ml; the antibiotic was changed to ceftriaxone when susceptibilities became available at 48 h. At 61 h, the aerobic blood culture bottle was flagged as positive. A Gram stain revealed spherical structures, which stained predominantly Gram-negative but were Gram-positive in some areas of the smear. Subcultures grew overnight on blood, chocolate and MacConkey agars and on brain–heart infusion (BHI) broth. Growth on MacConkey agar was sparse. A Gram stain of the snow-white, mucoid colonies revealed Gram-negative rods, spherical aggregates and thick-walled tetrads. Subsequent cultures on solid medium grew only Gram-negative rods, but inoculation of isolated colonies from these cultures into BHI broth or negative blood culture bottles caused the thick-walled spheres and tetrads to reappear.

Conclusion:

MicroScan and matrix-assisted laser desorption/ionization time of flight failed to identify the organism, but 16S rRNA gene sequencing identified it as genomospecies 5 (100 % match). To the best of our knowledge, there are no reports in the literature of aggregate-forming .

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2015-08-01
2020-01-25
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References

  1. I., Rolston K.V.I., Han X.Y.. 2004; Clinical significance of Roseomonas species isolated from catheter and blood samples: analysis of 36 cases in patients with cancer. Clin Infect Dis38:1579–1584 [CrossRef][PubMed]
    [Google Scholar]
  2. Gilardi G.L., Faur Y.C.. 1984; Pseudomonas mesophilica and an unnamed taxon, clinical isolates of pink-pigmented oxidative bacteria. J Clin Microbiol20:626–629[PubMed]
    [Google Scholar]
  3. Rihs J.D., Brenner D.J., Weaver R.E., Steigerwalt A.G., Hollis D.G., Yu V.L.. 1993; Roseomonas, a new genus associated with bacteremia and other human infections. J Clin Microbiol31:3275–3283[PubMed]
    [Google Scholar]
  4. Wang C.M., Lai C.C., Tan C.K., Huang Y.C., Chung K.P., Lee M.R., Hwang K.P., Hsueh P.R.. 2012; Clinical characteristics of infections caused by Roseomonas species and antimicrobial susceptibilities of the isolates. Diagn Microbiol Infect Dis72:199–203 [CrossRef][PubMed]
    [Google Scholar]
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