1887

Abstract

This study characterized the genetic basis of antimicrobial resistance of a number of spp. isolated from humans from 2000 to 2004 in Hiroshima prefecture, Japan. A total of 26 isolates of spp. were included in this study. Antimicrobial susceptibility tests revealed high levels of resistance, especially to ampicillin, streptomycin, trimethoprim, tetracycline, nalidixic acid and ciprofloxacin. PCR and DNA sequencing were used for screening and characterization of antibiotic-resistance determinants. PCR sequencing analysis revealed the presence of only one type of class 1 integron in one isolate of . This class 1 integron was 1904 bp and contained two gene cassettes: a probable esterase/lipase () and , which confers resistance to streptomycin and spectinomycin. Two types of class 2 integron were identified in this study. One was the classic type (2158 bp) and carried the three conserved resistance gene cassettes of the class 2 integron, , and , which confer resistance to trimethoprim, streptothricin and streptomycin/spectinomycin, respectively. This type was detected in both (14 isolates) and (five isolates). The other type was shorter (1313 bp) and carried only two gene cassettes, and . This integron was detected in a single isolate of . PFGE patterns showed limited diversity within clusters of the same species. Furthermore, an extended-spectrum -lactamase gene, , which confers resistance to ampicillin, was characterized in all isolates of except the oldest strain, which was isolated in 2000. Southern blot hybridization and conjugation experiments showed that was located in the chromosome.

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2006-12-01
2019-11-13
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