1887

Abstract

In the study, group B streptococci (GBS) isolated from bovines and humans in and around Van, eastern Turkey, were serotyped, and their haemagglutination and lectin-agglutination properties were also determined. This study is the first epidemiological survey of GBS serotypes performed in Turkey. A total of 148 GBS isolates, 76 from bovine milk and 72 from women attending a maternity polyclinic, were examined by co-agglutination, slide haemagglutination and slide lectin-agglutination tests. By the co-agglutination test, 34 (44·7 %) of bovine isolates and 49 (68 %) of human isolates could be serotyped. In bovine isolates, type VII (11·8 %), III (10·5 %), Ic (6·5 %) and VIII (3·9 %) were the most frequently detected serotypes. The most frequent human serotypes were Ic (33·3 %), IV (8·3 %), VIII (6·9 %), V (5·5 %) and R (5·5 %). In the haemagglutination test using rabbit erythrocytes, 23 (33·3 %) bovine and 15 (23·4 %) human isolates were found to be positive. The bovine GBS isolates showed a significant positive agglutination reaction with lectin (30·4 %), whereas the human GBS isolates were found to be positive for (18·8 %) and (37·5 %) lectins. The treatment of GBS with trypsin was also found to be important for the demonstration of the haemagglutination and lectin-agglutination properties of GBS. The results of the study provide data on serotype distribution and the formulation of a possible GBS vaccine in Turkey, and the lectin-agglutination tests may also be useful for differentiating bovine and human GBS strains.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/jmm.0.46156-0
2006-05-01
2019-10-19
Loading full text...

Full text loading...

/deliver/fulltext/jmm/55/5/517.html?itemId=/content/journal/jmm/10.1099/jmm.0.46156-0&mimeType=html&fmt=ahah

References

  1. Ainsworth, A. J. & Capley, G. ( 1986; ). Monoclonal antibodies produced to Streptococcus agalactiae. Am J Vet Res 47, 1211–1213.
    [Google Scholar]
  2. Berg, S., Trollfors, B., Lagergard, T., Zackrisson, G. & Claesson, A. ( 2000; ). Serotypes and clinical manifestations of group B streptococcal infections in Western Sweden. Clin Microbiol Infect 6, 9–13.
    [Google Scholar]
  3. Bopp, V. & Lämmler, C. ( 1995; ). Comparative studies on group-B streptococci isolated from bovine milk samples in Thuringia and Hesse. Zentralbl Veterinarmed B 42, 427–433.
    [Google Scholar]
  4. Christensen, P., Kahlmeter, G., Jonsson, S. & Kronvall, G. ( 1973; ). New method for the serological grouping of streptococci with specific antibodies adsorbed to protein A-containing staphylococci. Infect Immun 7, 881–885.
    [Google Scholar]
  5. Devriese, L. A. ( 1991; ). Streptococcal ecovars associated with different animal species: epidemiological significance of serogroups and biotypes. J Appl Bacteriol 71, 478–483.[CrossRef]
    [Google Scholar]
  6. Duarte, R. S., Miranda, O. P., Bellei, B. C., Brito, M. A. V. P. & Teixeira, L. M. ( 2004; ). Phenotypic and molecular characteristics of Streptococcus agalactiae isolates recovered from milk of dairy cows in Brazil. J Clin Microbiol 42, 4214–4222.[CrossRef]
    [Google Scholar]
  7. Finch, L. A. & Martin, D. R. ( 1984; ). Human and bovine group B streptococci: two distinct populations. J Appl Bacteriol 57, 273–278.[CrossRef]
    [Google Scholar]
  8. Harrison, L. H., Dwyer, D. M. & Johnson, J. A. ( 1995; ). Emergence of serotype V group B streptococcal infection among infants and adults. J Infect Dis 171, 513.[CrossRef]
    [Google Scholar]
  9. Jelinkova, J. ( 1977; ). Group B streptococci in the human population. Curr Top Microbiol Immunol 76, 127–165.
    [Google Scholar]
  10. Keefe, G. P. ( 1997; ). Streptococcus agalactiae mastitis: a review. Can Vet J 38, 429–437.
    [Google Scholar]
  11. Kellens, J. T., Jacobs, J. A., Peumans, W. J. & Stobberingh, E. E. ( 1993; ). The agglutination of beta-haemolytic streptococci by lectins. J Med Microbiol 39, 440–445.[CrossRef]
    [Google Scholar]
  12. Ko, W. C., Lee, H. C., Wang, L. R., Lee, C. T., Liu, A. J. & Wu, J. J. ( 2001; ). Serotyping and antimicrobial susceptibility of group B streptococcus over an eight-year period in southern Taiwan. Eur J Clin Microbiol Infect Dis 20, 334–339.
    [Google Scholar]
  13. Koneman, E. W., Allen, S. D., Dowell, V. R., Janda, W. M., Schreckenberger, P. C. & Winn, W. C. ( 1997; ). The Gram-positive cocci, part II: streptococci, enterococci and streptococcus-like bacteria. In Color Atlas and Text Book of Diagnostic Microbiology, 5th edn, pp. 577–629. Philadelphia, New York: Lippincott.
  14. Korhonen, T. K. & Finne, J. ( 1985; ). Agglutination assays for detecting bacterial binding specificities. In Enterobacterial Surface Antigens: Methods for Molecular Characterization, pp. 301–313. Edited by T. K. Korhonen, E. A. Dawes & P. H. Măkelă. Amsterdam: Elsevier.
  15. Kurl, D. N., Haataja, S. & Finne, J. ( 1989; ). Hemagglutination activities of group B, C, D and G streptococci: demonstration of novel sugar-specific cell-binding activities in Streptococcus suis. Infect Immun 57, 384–389.
    [Google Scholar]
  16. Kutschke, V. C. ( 1986; ). Zur serologischen Typendifferenzierung von Streptokokken der Gruppe B. Monatsh Vetmed 41, 227–228.
    [Google Scholar]
  17. Mosabi, J. M., Arimi, S. M. & Kangethe, E. K. ( 1997; ). Isolation and characterization of group B streptococci from human and bovine sources within and around Nairobi. Epidemiol Infect 118, 215–220.[CrossRef]
    [Google Scholar]
  18. Motlova, J., Wagner, M. & Jelinkova, J. ( 1986; ). A search for new group B streptococcal serotypes. J Med Microbiol 22, 101–105.[CrossRef]
    [Google Scholar]
  19. Moyo, S. R., Maeland, J. A. & Bergh, K. ( 2002; ). Typing of human isolates of Streptococcus agalactiae (group B streptococcus, GBS) strains from Zimbabwe. J Med Microbiol 51, 595–600.
    [Google Scholar]
  20. Munoz, A., Lopez, A. & Llovo, J. ( 1994; ). Lectin typing of beta-haemolytic streptococci of groups A and B. J Med Microbiol 41, 324–328.[CrossRef]
    [Google Scholar]
  21. Niewerth, B. ( 1987; ). Wechselwirkungen zwischen Gram-positiven Bakterien, Lektinen und Körperzellen. Dissertation, Fachbereich Veterinärmedizin der Justus Liebig Universität, Gießen.
  22. Ottensooser, F., Nakamizo, Y., Sato, M., Miyamoto, Y. & Takizawa, K. ( 1974; ). Lectin detecting group C streptococci. Infect Immun 9, 971–973.
    [Google Scholar]
  23. Pasaribu, F. H., Lämmler, C. & Blobel, H. ( 1985; ). Serotyping of bovine and human group B streptococci by coagglutination. IRCS (Int Res Commun Syst) Med Sci 13, 24–25.
    [Google Scholar]
  24. Schaefer, R. L., Keller, K. F. & Doyle, R. J. ( 1979; ). Lectins in diagnostic microbiology: use of wheat germ agglutinin for laboratory identification of Neisseria gonorrhoeae. J Clin Microbiol 10, 669–672.
    [Google Scholar]
  25. Schaufuss, P., Lämmler, C. & Blobel, H. ( 1986; ). Rapid differentiation of streptococci isolated from cows with mastitis. J Clin Microbiol 24, 1098–1099.
    [Google Scholar]
  26. Schuctat, A. & Wenger, J. D. ( 1994; ). Epidemiology of group B streptococcal disease. Epidemiol Rev 16, 374–402.
    [Google Scholar]
  27. Shet, A. & Ferrieri, P. ( 2004; ). Neonatal & maternal group B streptococcal infections: a comprehensive review. Indian J Med Res 120, 141–150.
    [Google Scholar]
  28. Slifkin, M. & Cumbie, R. ( 1987; ). Identification of Group B streptococcal antigen with lectin-bound polystyrene particles. J Clin Microbiol 25, 1172–1175.
    [Google Scholar]
  29. Slifkin, M. & Gil, G. M. ( 1983; ). Rapid biochemical tests for identification of group A, B, C, F and G streptococci from throat cultures. J Clin Microbiol 18, 29–32.
    [Google Scholar]
  30. Spellerberg, B. ( 2000; ). Pathogenesis of neonatal Streptococcus agalactiae infections. Microbes Infect 2, 1733–1742.[CrossRef]
    [Google Scholar]
  31. Swenshon, M., Lämmler, C. & Siebert, U. ( 1998; ). Identification and molecular characterization of beta-hemolytic streptococci isolated from harbour porpoises (Phocoena phocoena) of the North and Baltic Seas. J Clin Microbiol 36, 1902–1906.
    [Google Scholar]
  32. Wibawan, I. W. T., Lämmler, C., Seleim, R. S. & Pasaribu, F. H. ( 1993; ). A haemagglutinating adhesin of group B streptococci isolated from cases of bovine mastitis mediates adherence to HeLa cells. J Gen Microbiol 139, 2173–2178.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/jmm.0.46156-0
Loading
/content/journal/jmm/10.1099/jmm.0.46156-0
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error