1887

Abstract

Veterinary medicine has focused recently on reptiles, due to the existence of captive collections in zoos and an increase in the acquisition of reptiles as pets. The protozoan parasite, can cause amoebiasis in various animal species and humans. Although amoebiasis disease is remarkably rare in most species of chelonians and crocodiles, these species may serve as species carriers that transmit parasites to susceptible reptile species, such as snakes and lizards, which can become sick and die. In this study, we identified the species in a population of healthy (disease-free) chelonians, and evaluated their diversity through the amplification and sequencing of a small subunit rDNA region. Using this procedure, three species were identified: in 4.76 % of chelonians, in 3.96 % and in 50 %. We did not detect mixed infections. Comparative analysis of the amplified region allowed us to determine the intra-species variations. The and strains isolated in this study did not exhibit marked differences with respect to the sequences reported in GenBank. The analysis of the isolates revealed three different subgroups (A, B and C). Although subgroups A and C were very similar, subgroup B showed a relatively marked difference with respect to subgroups A and C (  = 0.984 and  = 1.000, respectively; 10–14 % nucleotide variation, as determined by ) and with respect to the sequences reported in GenBank. These results suggested that subgroup B may be either in a process of speciation or belong to a different lineage. However, additional research is necessary to support this statement conclusively.

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2014-02-01
2019-10-22
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