1887

Abstract

is a major causative agent of diarrhoeal disease worldwide in the human population. In contrast, heavy colonization of poultry typically does not lead to disease and colonized chickens are a major source of infections in humans. Previously, we have shown that chicken (but not human) intestinal mucus inhibits internalization. In this study, we test the hypothesis that chicken mucin, the main component of mucus, is responsible for this inhibition of virulence. Purified chicken intestinal mucin attenuated binding and internalization into HCT-8 cells depending on the site of origin of the mucin (large intestine>small intestine>caecum). invasion of HCT-8 cells was preferentially inhibited compared to bacterial binding to cells. Exposure of the mucin to sodium metaperiodate recovered bacterial invasion levels, suggesting a glycan-mediated effect. However, fucosidase or sialidase pre-treatment of mucin failed to abrogate the inhibition of pathogenicity. In conclusion, differences in the composition of chicken and human intestinal mucin may contribute to the differential outcome of infection of these hosts.

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2010-08-01
2019-10-22
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