1887

Abstract

The gene has rarely been reported in strains and its genetic environment has not yet been investigated.

To identify the gene in isolated from swine and characterize its genetic environment.

A isolate (named MM1L5) from a deceased swine was identified using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) and subjected to antimicrobial susceptibility testing. The genes were detected and then the genetic location and environment of were investigated by Southern blot and PCR mapping, respectively. The gene was cloned and expressed in .

Isolate MM1L5 harboured the and genes. The gene, located on the chromosome, was co-carried with an IS and gene by a novel 6361 bp IS-flanked composite transposon, designated Tn. This transposon consisted of a novel -containing module, IS-ΔIS -IS (named Tn), and a -containing module, IS -tnpR-IS, differing from previous reports. Phylogenetic analysis showed a significant variation based on the sequence of Tn, as compared to those of other related transposons. Interestingly, although the cloned gene could confer resistance to ceftiofur, cefquinome, ceftriaxone and cefotaxime, one amino acid substitution (Ile-142-Thr) resulted in a significant reduction of resistance to these antimicrobials.

This is the first time that has been identified on a chromosome from a isolate. Furthermore, the gene was located with an IS element and gene on a novel IS-flanked composite transposon, Tn, suggesting that Tn might act as a reservoir for the and genes and may become an important vehicle for their dissemination among .

Funding
This study was supported by the:
  • Gong Zheng Hu , National Key Research and Development Program of China , (Award 2016YFD05101304)
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/content/journal/jmm/10.1099/jmm.0.001235
2020-07-21
2020-09-20
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References

  1. Lewis JS, Herrera M, Wickes B, Patterson JE, Jorgensen JH. First report of the emergence of CTX-M-type extended-spectrum beta-lactamases (ESBLs) as the predominant ESBL isolated in a U.S. health care system. Antimicrob Agents Chemother 2007; 51:4015–4021 [CrossRef][PubMed]
    [Google Scholar]
  2. Pitout JDD, Laupland KB. Extended-Spectrum β-lactamase-producing Enterobacteriaceae: an emerging public-health concern. Lancet Infect Dis 2008; 8:159–166 [CrossRef][PubMed]
    [Google Scholar]
  3. Cantón R, Coque TM. The CTX-M beta-lactamase pandemic. Curr Opin Microbiol 2006; 9:466–475 [CrossRef][PubMed]
    [Google Scholar]
  4. Mugnaioli C, Luzzaro F, De Luca F, Brigante G, Amicosante G et al. Dissemination of CTX-M-type extended-spectrum beta-lactamase genes to unusual hosts. J Clin Microbiol 2005; 43:4183–4185 [CrossRef][PubMed]
    [Google Scholar]
  5. Hu X, Gou J, Guo X, Cao Z, Li Y et al. Genetic contexts related to the diffusion of plasmid-mediated CTX-M-55 extended-spectrum beta-lactamase isolated from Enterobacteriaceae in China. Ann Clin Microbiol Antimicrob 2018; 17:12 [CrossRef][PubMed]
    [Google Scholar]
  6. Partridge SR, Kwong SM, Firth N, Jensen SO. Mobile genetic elements associated with antimicrobial resistance. Clin Microbiol Rev 2018; 31:e00088–00017 [CrossRef][PubMed]
    [Google Scholar]
  7. Adeolu M, Alnajar S, Naushad S, S Gupta R. Genome-based phylogeny and taxonomy of the 'Enterobacteriales': proposal for Enterobacterales ord. nov. divided into the families Enterobacteriaceae, Erwiniaceae fam. nov., Pectobacteriaceae fam. nov., Yersiniaceae fam. nov., Hafniaceae fam. nov., Morganellaceae fam. nov., and Budviciaceae fam. nov. Int J Syst Evol Microbiol 2016; 66:5575–5599 [CrossRef][PubMed]
    [Google Scholar]
  8. O'Hara CM, Brenner FW, Miller JM. Classification, identification, and clinical significance of Proteus, Providencia, and Morganella. Clin Microbiol Rev 2000; 13:534–546 [CrossRef][PubMed]
    [Google Scholar]
  9. Bonnet R. Growing group of extended-spectrum beta-lactamases: the CTX-M enzymes. Antimicrob Agents Chemother 2004; 48:1–14 [CrossRef][PubMed]
    [Google Scholar]
  10. Metan G, Gulmez D, Eser OK, Kocagöz S, Sardan YC et al. CTX-M-3-type extended-spectrum beta-lactamase-producing Morganella morganii: first description of an isolate from Turkey. Int J Antimicrob Agents 2007; 30:368–370 [CrossRef][PubMed]
    [Google Scholar]
  11. Clinical and Laboratory Standards Institute Performance Standards for Antimicrobial Susceptibility Testing, 28th ed. Wayne, PA: CLSI Supplement M100; 2018
    [Google Scholar]
  12. Clinical and Laboratory Standards Institute Performance Standards for Antimicrobial Disk and Dilution Susceptibility Tests for Bacteria Isolated from Animals, 2nd ed. Wayne, PA: VET01. CLSI; 2018
    [Google Scholar]
  13. Stock I, Wiedemann B. Identification and natural antibiotic susceptibility of Morganella morganii. Diagn Microbiol Infect Dis 1998; 30:153–165 [CrossRef][PubMed]
    [Google Scholar]
  14. Dallenne C, Da Costa A, Decré D, Favier C, Arlet G. Development of a set of multiplex PCR assays for the detection of genes encoding important beta-lactamases in Enterobacteriaceae. J Antimicrob Chemother 2010; 65:490–495 [CrossRef][PubMed]
    [Google Scholar]
  15. Kumar S, Stecher G, Tamura K. MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol 2016; 33:1870–1874 [CrossRef][PubMed]
    [Google Scholar]
  16. Schultz E, Barraud O, Madec J-Y, Haenni M, Cloeckaert A et al. Multidrug Resistance Salmonella Genomic Island 1 in a Morganella morganii subsp. morganii Human Clinical Isolate from France. mSphere 2017; 2:e00118–00117 [CrossRef][PubMed]
    [Google Scholar]
  17. Jiang W, Men S, Kong L, Ma S, Yang Y et al. Prevalence of plasmid-mediated fosfomycin resistance gene fosA3 among CTX-M-Producing Escherichia coli isolates from chickens in China. Foodborne Pathog Dis 2017; 14:210–218 [CrossRef][PubMed]
    [Google Scholar]
  18. Lee S-Y, Park Y-J, Yu JK, Jung S, Kim Y et al. Prevalence of acquired fosfomycin resistance among extended-spectrum β-lactamase-producing Escherichia coli and Klebsiella pneumoniae clinical isolates in Korea and IS26-composite transposon surrounding fosA3. J Antimicrob Chemother 2012; 67:2843–2847 [CrossRef][PubMed]
    [Google Scholar]
  19. Fu L, Wang S, Zhang Z, Yan X, Yang X et al. Co-carrying of KPC-2, NDM-5, CTX-M-3 and CTX-M-65 in three plasmids with serotype O89: H10 Escherichia coli strain belonging to the ST2 clone in China. Microb Pathog 2019; 128:1–6 [CrossRef][PubMed]
    [Google Scholar]
  20. Dhanji H, Doumith M, Rooney PJ, O'Leary MC, Loughrey AC et al. Molecular epidemiology of fluoroquinolone-resistant ST131 Escherichia coli producing CTX-M extended-spectrum beta-lactamases in nursing homes in Belfast, UK. J Antimicrob Chemother 2011; 66:297–303 [CrossRef][PubMed]
    [Google Scholar]
  21. Woodford N, Carattoli A, Karisik E, Underwood A, Ellington MJ et al. Complete nucleotide sequences of plasmids pEK204, pEK499, and pEK516, encoding CTX-M enzymes in three major Escherichia coli lineages from the United Kingdom, all belonging to the international O25:H4-ST131 clone. Antimicrob Agents Chemother 2009; 53:4472–4482 [CrossRef][PubMed]
    [Google Scholar]
  22. Harmer CJ, Moran RA, Hall RM. Movement of IS26-associated antibiotic resistance genes occurs via a translocatable unit that includes a single IS26 and preferentially inserts adjacent to another IS26. mBio 2014; 5:e01801–01814 [CrossRef][PubMed]
    [Google Scholar]
  23. Harmer CJ, Hall RM. IS26-Mediated formation of transposons carrying antibiotic resistance genes. mSphere 2016; 1:e00038–00016 [CrossRef][PubMed]
    [Google Scholar]
  24. Haroche J, Allignet J, El Solh N. Tn5406, a new staphylococcal transposon conferring resistance to streptogramin A and related compounds including dalfopristin. Antimicrob Agents Chemother 2002; 46:2337–2343 [CrossRef][PubMed]
    [Google Scholar]
  25. Kehrenberg C, Schwarz S. Florfenicol-chloramphenicol exporter gene fexA is part of the novel transposon Tn558. Antimicrob Agents Chemother 2005; 49:813–815 [CrossRef][PubMed]
    [Google Scholar]
  26. Kadlec K, Schwarz S. Identification of the novel dfrK-carrying transposon Tn559 in a porcine methicillin-susceptible Staphylococcus aureus ST398 strain. Antimicrob Agents Chemother 2010; 54:3475–3477 [CrossRef][PubMed]
    [Google Scholar]
  27. Xu J, Jia H, Cui G, Tong H, Wei J et al. ICEAplChn1, a novel SXT/R391 integrative conjugative element (ICE), carrying multiple antibiotic resistance genes in Actinobacillus pleuropneumoniae. Vet Microbiol 2018; 220:18–23 [CrossRef][PubMed]
    [Google Scholar]
  28. He DD, Zhao SY, Wu H, Hu GZ, Zhao JF et al. Antimicrobial resistance-encoding plasmid clusters with heterogeneous MDR regions driven by IS26 in a single Escherichia coli isolate. J Antimicrob Chemother 2019; 74:1511–1516 [CrossRef][PubMed]
    [Google Scholar]
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