1887

Abstract

The gene, one of the 16S rRNA methylase genes whose products confer high-level resistance to aminoglycosides, is most prevalent among strains. In this study, eight non-duplicate -carrying avian strains from a farm in China were isolated and characterized, and further examined by phylogenetic grouping, conjugation experiments and PCR-based replicon typing. In addition, the genetic environment of was investigated by cloning and sequencing. Six -carrying were identified as phylogroup A, sequence type (ST) 156 (A-ST156), with two assigned to D-ST117; however, all of them carried the same IncI1 ST136 plasmid. The genetic environment of the gene in these eight plasmids was the same, as shown by PCR mapping. A multidrug-resistant region carrying , , a class 1 integron cassette array (---Δ-) and was characterized on the conjugative IncI1 ST136 plasmid. Co-location of the gene with a class 1 integron cassette array and on the conjugative plasmid will facilitate its maintenance and dissemination.

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2016-05-01
2019-10-15
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