Heated saline extracts of 89 strains, and (1) supernates of phenol-water extracts (L fractions), (2) purified lipopolysaccharide, (3) trichloracetic-acid (TCA) extracts, and (4) sodium-hydroxide extracts of 23 strains representing all O antigens were subjected to electrophoresis. Precipitation lines obtained with homologous and heterologous antisera were evaluated by electrodensitometric measurement. The characteristics of the immunoelectrophoretic groups established were as follows. : two lines running at different rates towards the anode; three subgroups on the basis of the behaviour of alkali-treated antigens. : triple line at the starting well, alkali sensitive. : triple line at the starting well, alkali resistant; two subgroups according to reactivity or non-reactivity of L fractions. : triple line on the cathode side, alkali resistant, L fraction non-reactive. : single line on the anode side, alkali sensitive, L fraction and TCA extract non-reactive. O antigens identified by agglutination corresponded closely with the immunoelectrophoretic pattern: strains with identical O antigens or sharing major somatic components fell, with one exception, into the same immunoelectrophoretic group.


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