1887

Abstract

This study was performed to differentiate serologically between patients with hydatid disease which is active, and which has been successfully cured. A total of 18 cases was included. Pre-treatment serum samples were collected before surgery or chemotherapy. Post-treatment serum samples were collected at various intervals (3 days, 7 days, 1 month, 6 months, 1 year and 2 years) after surgery or chemotherapy. These sera were tested for the presence of circulating hydatid antigen (CAg) by bacterial co-agglutination (Co-A) and counter-current immunoelectrophoresis (CIEP) tests, and for circulating hydatid antibodies (CAb) by indirect haemagglutination assay (IHA). Ten and eight sera, respectively, were positive out of 11 pre-operative and pre-chemotherapeutic sera tested for CAg by the Co-A and CIEP tests. Post-operative sera collected from these cases did not show any CAg by the CIEP test. However, CAg was detected by Co-A in three and four serum samples collected on the third and seventh day, respectively, after surgical removal of the cyst. However, the CAg levels in these post-operative sera showed a gradual decline by the seventh day and were completely absent in the serum specimens collected 1 month after surgery and 6 months after chemotherapy. All the post-operative serum samples except.two, collected 2 years after surgical removal of the cyst, in seven cases of old hydatid disease, were negative for CAg by both the CIEP and Co-A tests. Unlike the CAg profile, no marked differences were noted between the CAb profile of the pre-and post-treatment sera, as shown by the IHA test. Even 1 year after surgery or chemotherapy, two sera showed a marginal decrease in their CAb titre. CAb at varying titres was still detectable in all seven serum samples from old cases of hydatid disease, even 2 years after surgical removal of the cyst. This study shows the value of serial pre-and post-operative or chemotherapy estimation of CAg by Co-A and CIEP as an index of cure or of continuing hydatid infection.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-46-10-859
1997-10-01
2024-12-04
Loading full text...

Full text loading...

/deliver/fulltext/jmm/46/10/medmicro-46-10-859.html?itemId=/content/journal/jmm/10.1099/00222615-46-10-859&mimeType=html&fmt=ahah

References

  1. Bhatia B. B., Pathak K. M. L. Echinococcosis. In Parija S. C. (ed) Review of parasitic zoonoses Delhi: AITBS Publishers and Distributors; 1990268–280
    [Google Scholar]
  2. Parija S. C., Rao R. S., Badrinath S., Sengupta D. N. Hydatid disease in Pondicherry. J Trop Med Hyg 1983; 66:113–115
    [Google Scholar]
  3. Parija S. C. Recent trends in the serodiagnosis of hydatid disease. Southeast Asian J Trop Med Public Health 1991; 22: Suppl 371–376
    [Google Scholar]
  4. Parija S. C. New approaches in the serodiagnosis of hydatid disease. J Indian Med Assoc 1994; 92:281–284
    [Google Scholar]
  5. Gottstein B. An immunoassay for the detection of circulating antigens in human echinococcosis. Am J Trop Med Hyg 1984; 33:1185–1191
    [Google Scholar]
  6. Shariff G. M., Parija S. C. Counter-current immunoelectrophoresis for serodiagnosis of hydatid disease by detection of circulating hydatid antigen. J Microbiol Methods 1991; 14:71–76
    [Google Scholar]
  7. Shariff G. M., Parija S. C. Co-agglutination (Co-A) test for circulating antigen in hydatid disease. J Med Microbiol 1993; 38:231–234
    [Google Scholar]
  8. Parija S. C., Ananthakrishnan N. Evaluation of stabilised cells in the indirect haemagglutination test for echinococcosis. J Med Microbiol 1985; 19:95–98
    [Google Scholar]
  9. Parija S. C., Rao R. S. Enhancement of sensitivity of the haemagglutination test for echinococcosis by use of Staphylococcus aureus protein A. J Med Microbiol 1986; 22:241–244
    [Google Scholar]
  10. Craig P. S., Nelson G. S. The detection of circulating antigen in human hydatid disease. Ann Trop Med Parasitol 1984; 78:219–227
    [Google Scholar]
  11. Kanwar J. R., Vinayak V. K. The significance of free and immuno-complexed hydatid-specific antigen(s) as an immuno-diagnostic tool for human hydatidosis. J Med Microbiol 1984; 37:396–403
    [Google Scholar]
  12. Parija S. C. Simple immunoassays in the serodiagnosis of hydatid disease at the field or poorly equipped laboratories. Indian J Med Microbiol 1993; 11:1–4
    [Google Scholar]
/content/journal/jmm/10.1099/00222615-46-10-859
Loading
/content/journal/jmm/10.1099/00222615-46-10-859
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error