1887

Abstract

Summary

The opsonic requirements of were investigated in a series of experiments with human polymorphonuclear leucocytes (PMNL). Pre-incubation of with pooled normal human serum (NHS) in concentrations of 5–20% significantly increased the uptake of radiolabelled bacteria by PMNL. Treatment of the bacteria with NHS 30% caused the release of radiolabel and this effect was abolished by heating serum to 56°C, suggesting that is serum-sensitive and that complement is involved. Opsonisation of with NHS concentrations of 10–30% significantly increased PMNL chemi-luminescence. Removal of specific antibody had no effect. Removal of either the classical or alternative complement pathways produced no significant change in PMNL chemilum-inescence, indicating that either pathway is sufficient for opsonisation on its own. The results confirm that complement is the most efficient opsonin for

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/content/journal/jmm/10.1099/00222615-38-3-209
1993-03-01
2020-01-24
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http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-38-3-209
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