1887

Abstract

. Thirty strains from the group (SMG) obtained from various sources were tested for a range of characters that could be associated with pathogenicity and the results were compared with those for type strains of , and . The SMG strains were heterogeneous in all tests. Most (18) belonged to one of the Lancefield groups with group F predominating. Adherence of strains isolated from abscesses to buccal epithelial cells was greater than that of other strains (p = 0.033). Compared with strains of , SMG strains were generally not aggregated by human saliva. They differed from the type strain of in their relative ability to bind fibrinogen and fibronectin; they were less effective in binding fibrinogen (0.33-4.28% cf. 22% for ) and generally more effective in binding fibronectin (0.49-12.37% cf. 0.95%). Strains isolated from infections were statistically better at binding fibronectin than other strains (p <0.001). The ability of strains to adhere to saliva-coated hydroxyapatite (SHA) varied 10-fold, from 0.16-16.35%. The amount of fibronectin bound by SMG strains correlated with their ability to adhere to SHA (p <0.001). The hydrophobicity of the strains, as measured in the hexadecane partition assay, ranged from 0.0% to 99.0%. Some strains carried both positive and negative cell-surface charges and some strains with a highly hydrophobic cell surface also possessed a relatively high cell-surface charge. A minority of strains possessed a net positive cell-surface charge. Neither hydrophobicity nor cell-surface charge was related to the capacity of strains to adhere to SHA. Strains of SMG co-aggregated weakly with strains of , V. dispar, and .

Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-31-4-259
1990-04-01
2024-12-06
Loading full text...

Full text loading...

/deliver/fulltext/jmm/31/4/medmicro-31-4-259.html?itemId=/content/journal/jmm/10.1099/00222615-31-4-259&mimeType=html&fmt=ahah

References

  1. Abraham S N, Beachey E H, Simpson W A. 1983; Adherence of Streptococcus pyogenes, Escherichia coli and Pseudomonas aeruginosa to fibronectin–coated and uncoated epithelial cells. Infection and Immunity 41:1261–12640
    [Google Scholar]
  2. Absolom D R. 1988; The role of bacterial hydrophobicity in infection: bacterial adhesion and phagocytic ingestion. Canadian Journal of Microbiology 34:287–298
    [Google Scholar]
  3. Babu J P, Dabbous M K. 1986; Interaction of salivary fibronectin with oral streptococci. Journalof Dental Research 65:1094–1100
    [Google Scholar]
  4. Babu J P, Simpson W A, Courtney H S, Beachey E H. 1983; Interaction of human plasma fibronectin with cariogenic and non–cariogenic oral streptococci. Infection and Immunity 41:116–216
    [Google Scholar]
  5. Cisar J O, Kolenbrander P E, McIntire F C. 1979; Specificity of coaggregation reactions between human oral streptococci and strains of Actinomyces ciscosusor Actinomycesnaeslundii. Infection and Immunity 24:474–275
    [Google Scholar]
  6. Coykendall A L, Wesbecher P M, Gustafson K B. 1987; “Streptococcus milleri”, Streptococcus constellatus and Streptococcus intermedius are later synonyms of Streptococcus anginosus. International Journal of Systematic Bacteriology 37:722–222
    [Google Scholar]
  7. de Louvois J. 1980; Bacteriological examination of pus from abscesses of the central nervous system. Journal of Clinical Pathology 33:66–71
    [Google Scholar]
  8. Drucker D B, Green R M . 1978; The relative cariogenicities of Streptococcus milleri and other viridans group streptococci in gnotobiotic hooded rats. Archives of Oral Biology 23:151–157
    [Google Scholar]
  9. Drucker D B, Lee S M. 1983; Possible heterogeneity of Streptococcus milleri determined by DNA and mol % (guanine plus cytosine) measurement and physiological characterisation. Microbios 38:815–115
    [Google Scholar]
  10. Ericson T, Olsson J, Bowen W, Ciardi J, Rundegren J. 1983; Effect of purified human salivary agglutinin on the adsorption of S. mutans to hydroxyapatite. ten Cate J. M. et al. Bacterial adhesion and preventative dentistry IRL Press; Oxford:63–71
    [Google Scholar]
  11. Ericson T, Pruit K, Wedel H. 1975; The reaction of salivary substances with bacteria. Journal of Oral Pathology 4:307–323
    [Google Scholar]
  12. Gibbons R J. 1984; Adherent interactions which may affect microbial ecology in the mouth. Journal of Dental Research. 63:337–838
    [Google Scholar]
  13. Gibbons R J. 1989; Bacterial adhesion to oral tissues: a model for infectious diseases. Journal of Dental Research 68:875–076
    [Google Scholar]
  14. Gibbons R J, van Houte J. 1975; Bacterial adherence in oral microbial ecology. Annual Review of Microbiology 29:19–44
    [Google Scholar]
  15. Gossling J. 1988; Occurrence and pathogenicity of the Streptococcus milleri group. Reviews of Infectious Diseases 10:025–728
    [Google Scholar]
  16. Handley P S, Harty D W S, Wyatt J E, Brown C R, Doran J P, Gibbs A C C. 1987; A comparison of the adhesion, coaggregation and cell–surface hydrophobicity properties of fibrillar and fimbriate strains of Streptococcus salivarius. Journal of General Microbiology 133:3207–3217
    [Google Scholar]
  17. Hoggy S D, Manning J E. 1989; Inhibition of adhesion of viridans streptococci to fibronectin coated hydroxyapatite beads by lipoteichoic acid. Journal of Applied Bacteriology 65:548–348
    [Google Scholar]
  18. Jenkinson H F, Carter D A. 1988; Cell surface mutants of Streptococcus sanguis with altered adherence properties. Oral Microbiology and Immunology 3:53–57
    [Google Scholar]
  19. Kilpper–Balz R, Williams B L, Lutticken R, Schleifer K H. 1984; Relatedness of “Streptococcus milleri” with Streptococcus anginosus and Streptococcus constellatus. Systematic and Applied Microbiology 5:494–500
    [Google Scholar]
  20. Knight R G, Shlaes D M. 1988; Physiological characteristics and deoxyri bonucleic acid relatedness of Streptococcus interrnedius strains. International Journal of Systematic Bacteriology 38:19–24
    [Google Scholar]
  21. Kolenbrander P E, Williams B L. 1983; Prevalence of viridans streptococci exhibiting lac tose–inhibi table coaggregation with oral actinomycetes. Infection and Immunity 41:144–945
    [Google Scholar]
  22. Mandel I D. 1976; Non–immunologic aspects of caries resistance. Journal of Dental Research 55:22–31
    [Google Scholar]
  23. Markwell M A. 1982; A new solid–state reagent to iodinate proteins. I. Conditions for the efficient labeling of antiserum. Analytical Biochemistry 125:2542–743
    [Google Scholar]
  24. McBride B C, van der Hoeven J S. 1981; Role of interbacterial adherence in colonization of the oral cavities of gnotobiotic rats infected with Streptococcus mutans and Veillonella alcalescens. Infection and Immunity 33:346–747
    [Google Scholar]
  25. Mejare B, Edwardsson S. 1975; Streptococcus milleri (Guthof); an indigenous organism of the human oral cavity. Archices of Oral Biology 20:075–776
    [Google Scholar]
  26. Michalek S M, McGhee J R. 1982 Oral streptococci with emphasis on Streptococcus mutans.. McGhee J R. Dental microbiology Harper and Row; Philadelphia:679–690
    [Google Scholar]
  27. Moore–Gillon J C, Eykyn S J, Phillips I. 1981; Microbiology of pyogenic live abscess. British Medical Journal 283:8381–982
    [Google Scholar]
  28. Poole P M, Wilson G. 1976; Infection with minute–colonyforming β–haemolytic streptococci. Journal of Clinical Pathology. 29:974–074
    [Google Scholar]
  29. Reynolds E C, Wong A. 1983; The effect of adsorbed protein on hydroxyapatite zeta potential and Streptococcus mutans adherence. Infection and Immunity 39:1285–12930
    [Google Scholar]
  30. Rogosa M. 1964; The genus Veillonella. I. General cultural, ecological and biochemical considerations. Journal of Bacteriology 87:716–217
    [Google Scholar]
  31. Rosenberg M, Gutnick D, Rosenberg E. 1980; Adherence of bacteria to hydrocarbons: a simple method for measuring cell–surface hydrophobicity. FEMS Microbiology Letters 9:29–33.
    [Google Scholar]
  32. Ruoff K L. 1988; Streptococcus anginosus (Streptococcus milleri): the unrecognized pathogen. Clinical Microbiology Reviews 1:102–108
    [Google Scholar]
  33. Ruoff K L, Ferraro M J. 1987; Hydrolytic enzymes of “Streptococcus milleri”. Journal of Clinical Microbiology 25:1645–16420
    [Google Scholar]
  34. Sklavounou A, Germaine G R. 1980; Adherence of oral streptococci to keratinized and non–keratinized human oral epithelial cells. Infection and Immunity 27:768–668
    [Google Scholar]
  35. Sokal R R, Rohlf F J. 1981; Biometry: the principles and practice of statistics in biomedical research. , 2. W. H. Freeman and Co; San Francisco:372–387
    [Google Scholar]
  36. Stenstrom T A. 1989; Bacterial hydrophobicity, an overall parameter for the measurement of adhesion potential to soil particles. Applied and Environmental Microbiology 55:142–147
    [Google Scholar]
  37. Weerkamp A H, McBride B C. 1981 Identification of a Streptococcus saliuarius cell wall component mediating coaggregation with Veillonella alcalescens V1,Infection and Immunity 32:272–373
    [Google Scholar]
  38. Willcox M D P, Drucker D B, Hillier V F, Green R M. 1985; Relationship of adhesion in vitro and cariogenicity of oral Streptococcus species in germ–free rats. Archives of Oral Biology 30:063–563
    [Google Scholar]
  39. Willcox M D P, Drucker D B. 1989; Surface structures, coaggregation and adherence phenomena of Streptococcus oralis and related species. Microbios 59:19–29
    [Google Scholar]
  40. Williams R C, Gibbons R J. 1975; Inhibition of streptococcal attachment to receptors on human buccal epithelial cells by antigenically similar salivary components. Infection and Immunity 11:171
    [Google Scholar]
  41. Whitnack E, Dale J B, Beachey E H. 1984; Common protective antigens of group A streptococcal M proteins masked by fibrinogen. Journal of Experimental Medicine 159:1201–1212
    [Google Scholar]
  42. Wyatt J E, Handley P S. 1987; Aggregation of Streptococcus sanguis biotypes I and II by parotid saliva: a comparison between peritrichously fibrillar and tufted strains. Microbios 51:111–312
    [Google Scholar]
  43. Wyatt J E, Hesketh L M, Handley P S. 1987; Lack of correlation between fibrils, hydrophobicity and adhesion for strains of Streptococcus sanguis biotypes I and II. Microbios 50:7–15
    [Google Scholar]
  44. Yakushiji T, Konagawa R, Oda M, Inoue M. 1988; Serological variation in oral Streptococcus milleri. Journal of Medical Microbiology 27:714–515
    [Google Scholar]
  45. Yang K D, Augustine N H, Gonzalez L A, Bohnsack J F, Hill H R. 1988; Effects of fibronectin on the interaction of polymorphonuclear leukocytes with unopsonised and antibody–opsonised bacteria. Journal of Infectious Diseases 158:5882–383
    [Google Scholar]
/content/journal/jmm/10.1099/00222615-31-4-259
Loading
/content/journal/jmm/10.1099/00222615-31-4-259
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error