1887

Abstract

Summary.

was examined for its haemagglutinating activity and adherence to cultured epithelial cells (HEp-2) in relation to possession of a virulence (VW) plasmid and to growth conditions. VW-lacking (VW) bacteria were isolated from ten VW strains of each serovar which, after they were grown on CFA plates at 37°C, agglutinated the erythrocytes from five different species. In contrast to the bacteria possessing the plasmid (VW) half of the VW bacteria, grown on CFA plates at 37°C, did not agglutinate any of the erythrocytes used and the other half agglutinated only human erythrocytes. Furthermore, when grown on CFA plates at 25°C, neither VW nor VW bacteria showed a haemagglutinating activity. When the bacteria were grown in CFA broth, only two strains grown at 25°C did not agglutinate any of the erythrocytes tested. The VW and VW bacteria of the remaining strains, grown either at 25°C or 37°C, showed relatively high haemagglutinating activity. Adherence to HEp-2 cells did not correlate with haemagglutinating activity in the VW bacteria grown at 37°C adhered to HEp-2 cells more efficiently than either the VW derivatives or the VW bacteria grown at 25°C, regardless of the growth medium. These results indicate that some of the haemagglutinins detected on are not involved in the adherence to HEp-2 cells.

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1988-03-01
2024-11-12
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References

  1. Aleksic S, Rohde R, Muller G, Wohlers B. 1976; Examination of the envelope antigen Kl in Yersinia enterocolitica which was identified as fimbriae. Zentralblatt fur Bakteriologie, Parasitenkunde, Infektionskrankheiten und Hygiene I, Abtei-lung Originale A 234:513–520
    [Google Scholar]
  2. Balligand G, Laroche Y, Cornells G. 1985; Genetic analysis of virulence plasmid from a serogroup 9 Yersinia enterocolitica strain: Role of outer membrane protein PI in resistance to human serum and autoagglutination. Infection and Immunity 48:782–786
    [Google Scholar]
  3. Bölin I, Norlander L, Wolf-Watz H. 1982; Temperature-inducible outer membrane protein of Yersinia pseudotuberculosis and Yersinia enterocolitica is associated with the virulence plasmid. Infection and Immunity 37:506–512
    [Google Scholar]
  4. Evans D G, Evans D J, Tjoa W S. 1977; Hemagglutination of human group A erythrocytes by enterotoxigenic Escherichia coli isolated from adults with diarrhea: correlation with colonization factor. Infection and Immunity 18:330–337
    [Google Scholar]
  5. Ferber D M, Brubaker R R. 1981; Plasmid in Yersinia pestis. Infection and Immunity 31:839–841
    [Google Scholar]
  6. Gemski P, Lazere J R, Casey T. 1980a; Plasmid associated with pathogenicity and calcium dependency of Yersinia enterocolitica. Infection and Immunity 27:682–685
    [Google Scholar]
  7. Gemski P, Lazere J R, Casey T, Wohlhieter J A. 19806; Presence of a virulence-associated plasmid in Yersinia pseudotuberculosis. Infection and Immunity 28:1044–1047
    [Google Scholar]
  8. Heesemann J, Algermissen B, Laufs R. 1984; Genetically manipulated virulence of Yersinia enterocolitica. Infection and Immunity 46:105–110
    [Google Scholar]
  9. Heesemann J, Keller C, Morawa R, Schmidt N, Siemens H J, Laufs R. 1983; Plasmids of human strains of Yersinia enterocolitica: molecular relatedness and possible importance for pathogenesis. Journal of Infectious Disease 147:107–115
    [Google Scholar]
  10. Higuchi K, Smith J L. 1961; Studies on the nutrition and physiology of Pasteurella pestis. VI. A differential plating medium for the estimation of the mutation rate to avirulence. Journal of Bacteriology 81:605–608
    [Google Scholar]
  11. Kapperud G, Lassen J. 1983; Relationship of virulence-associated autoagglutination to hemagglutinin production in Yersinia enterocolitica and Yersinia enterocolitica-like bacteria. Infection and Immunity 42:163–169
    [Google Scholar]
  12. Kapperud G, Namork E, Skarpeid H J. 1985a; Temperature-inducible surface fibrillae associated with the virulence plasmid of Yersinia enterocolitica and Yersinia pseudotuberculosis. Infection and Immunity 47:561–566
    [Google Scholar]
  13. Kapperud G, Skarpeid H J, Solberg R, Bergan T. 1985b; Outer membrane proteins and plasmids in different Yersinia enterocolitica serogroups isolated from man and animals. Acta Pathologica Microbiologica et Immunologica Scandinav-ica Section B 93:27–35
    [Google Scholar]
  14. Kawaoka Y, Otsuki K, Tsubokura M. 1982; Characteristics of Yersinia enterocolitica bacteriophages. Zentralblatt fur Bak-teriologie Mikrobiologie und Hygiene I Abteilung Originale A 253:102–109
    [Google Scholar]
  15. Kawaoka Y, Otsuki K, Tsubokura M. 1983; Growth temperature-dependent variation in the bacteriophage-inactivating capacity and antigenicity of Yersinia enterocolitica lipo-polysaccharide. Journal of General Microbiology 129:2739–2747
    [Google Scholar]
  16. Kihlstrom E, Magnusson K E. 1983; Hemagglutinating, adhesive and physico-chemical surface properties of different Yersinia enterocolitica and Yersinia enterocolitica-like bacteria. Acta Pathologica Microbiologica et Immunologica Scandinav-ica Section B 91:113–119
    [Google Scholar]
  17. Lachica R V, Zink D L. 1984a; Plasmid-associated cell surface charge and hydrophobicity of Yersinia enterocolitica. Infection and Immunity 44:540–543
    [Google Scholar]
  18. Lachica R V, Zink D L. 1984b; Determination of plasmid-associated hydrophobicity of Yersinia enterocolitica by a latex particle agglutination test. Journal of Clinical Microbiology 19:660–663
    [Google Scholar]
  19. Lachica R V, Zink D L, Ferris W R. 1984; Association of fibril structure formation with cell surface properties of Yersinia enterocolitica. Infection and Immunity 46:272–275
    [Google Scholar]
  20. Laird W J, Cavanaugh D C. 1980; Correlation of autoagglutina-tion and virulence of Yersiniae. Journal of Clinical Microbiology 11:430–432
    [Google Scholar]
  21. Lee W H, Smith R E, Damare J M, Harris M E, Johnston R W. 1981; Evaluation of virulence test procedures for Yersinia enterocolitica recovered from foods. Journal of Applied Bacteriology 50:529–539
    [Google Scholar]
  22. Maclagan R M, Old D C. 1980; Hemagglutinins and fimbriae in different serotypes and biotypes of Yersinia enterocolitica. Journal of Applied Bacteriology 49:353–360
    [Google Scholar]
  23. Martinez R J. 1983; Plasmid-mediated and temperature-regulated surface properties of Yersinia enterocolitica. Infection and Immunity 41:921–930
    [Google Scholar]
  24. Okamoto K, Inoue T, Ichikawa H, Kawamoto Y, Hara S, Miyama A. 1980; Adherence of Yersinia enterocolitica to mammalian epithelial cell lines. Microbiology and Immunology 24:1013–1022
    [Google Scholar]
  25. Old D C, Robertson J. 1981; Adherence of fimbriate and non-fimbriate strains of Yersinia enterocolitica to human epithelial cells. Microbiology and Immunology 25:993–998
    [Google Scholar]
  26. Portnoy D A, Falkow S. 1981; Virulence-associated plasmids from Yersinia enterocolitica and Yersinia pestis. Journal of Bacteriology 148:877–883
    [Google Scholar]
  27. Portnoy D A, Moseley S L, Falkow S. 1981; Characterization of plasmids and plasmid-associated determinants of Yersinia enterocolitica pathogenesis. Infection and Immunity 31:775–782
    [Google Scholar]
  28. Schiemann D A. 1981; An enterotoxin-negative strain of Yersinia enterocolitica serotype 0:3 is capable of producing diarhea in mice. Infection and Immunity 32:571–574
    [Google Scholar]
  29. Skurnik M. 1984; Lack of correlation between the presence of plasmids and fimbriae in Yersinia enterocolitica and Yersinia pseudotuberculosis. Journal of Applied Bacteriology 56:355–363
    [Google Scholar]
  30. Skurnik M, Bolin I, Heikkinen H, Piha S, Wolf-Watz H. 1984; Virulence plasmid-associated autoagglutination in Yersinia spp. Journal of Bacteriology 158:1033–1036
    [Google Scholar]
  31. Straley S C, Brubaker R R. 1981; Cytoplasmic and membrane proteins of Yersiniae cultivated under conditions simulating mammalian intracellular environment. Proceedings of the National Academy of Sciences of the USA 78:1224–1228
    [Google Scholar]
  32. Straley S C, Brubaker R R. 1982; Localization in Yersinia pestis of peptides associated with virulence. Infection and Immunity 36:129–135
    [Google Scholar]
  33. Tsubokura M, Otsuki K, Kawaoka Y, Fukushima H, Ikemura K, and Kanazawa Y. 1984; Addition of new serogroups and improvement of the antigenic designs of Yersinia pseudo-tuberculosis. Current Microbiology 11:89–92
    [Google Scholar]
  34. Vesikari T. 1981; Plasmids in Yersinia enterocolitica serotypes 0:3 and 0:9: correlation with epithelial cell adherence in vitro. Infection and Immunity 33:870–876
    [Google Scholar]
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