was examined for its haemagglutinating activity and adherence to cultured epithelial cells (HEp-2) in relation to possession of a virulence (VW) plasmid and to growth conditions. VW-lacking (VW) bacteria were isolated from ten VW strains of each serovar which, after they were grown on CFA plates at 37°C, agglutinated the erythrocytes from five different species. In contrast to the bacteria possessing the plasmid (VW) half of the VW bacteria, grown on CFA plates at 37°C, did not agglutinate any of the erythrocytes used and the other half agglutinated only human erythrocytes. Furthermore, when grown on CFA plates at 25°C, neither VW nor VW bacteria showed a haemagglutinating activity. When the bacteria were grown in CFA broth, only two strains grown at 25°C did not agglutinate any of the erythrocytes tested. The VW and VW bacteria of the remaining strains, grown either at 25°C or 37°C, showed relatively high haemagglutinating activity. Adherence to HE-2 cells did not correlate with haemagglutinating activity in ; the VW bacteria grown at 37°C adhered to HE-2 cells more efficiently than either the VW derivatives or the VW bacteria grown at 25°C, regardless of the growth medium. These results indicate that some of the haemagglutinins detected on are not involved in the adherence to HE-2 cells.


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