1887

Abstract

SUMMARY. Antigens obtained from the ruptured mycelium of were separated on the basis of their attachment or non-attachment to Concanavalin A-linked Sepharose gel. They were analysed by double-diffusion and two-dimensional immunoelectrophoresis with rabbit antiserum and sera from patients with aspergillus-related disease. Their sensitivity was assessed in an enzyme-linked immunosorbent assay (ELISA) for immunoglobulin G. Sera which gave very low or high extinction readings on ELISA gave, in general, comparable results on counter-immunoelectrophoresis; but weak electrophoretic reactions were not closely paralleled by ELISA results. The unfractionated extract and the fraction bound by Concanavalin A-Sepharose had similar ability to detect specific IgG in sera from patients with aspergillosis.

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/content/journal/jmm/10.1099/00222615-16-1-97
1983-02-01
2020-01-24
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http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-16-1-97
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