1887

Abstract

Nipah virus (NiV) is a recently emergent, highly pathogenic, zoonotic paramyxovirus of the genus . Like the phosphoprotein (P) gene of other paramyxoviruses, the P gene of NiV is predicted to encode three additional proteins, C, V and W. When the C, V and W proteins of NiV were tested for their ability to inhibit expression of the chloramphenicol acetyltransferase (CAT) reporter gene in plasmid-based, minigenome replication assays, each protein inhibited CAT expression in a dose-dependent manner. The C, V and W proteins of NiV also inhibited expression of CAT from a measles virus (MV) minigenome, but not from a human parainfluenzavirus 3 (hPIV3) minigenome. Interestingly, the C and V proteins of MV, which have previously been shown to inhibit MV minigenome replication, also inhibited NiV minigenome replication; however, they were not able to inhibit hPIV3 minigenome replication. In contrast, the C protein of hPIV3 inhibited minigenome replication of hPIV3, NiV and MV. Although there is very limited amino acid sequence similarity between the C, V and W proteins within the paramyxoviruses, the heterotypic inhibition of replication suggests that these proteins may share functional properties.

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2008-05-01
2019-11-22
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References

  1. Atreya, P. L., Peeples, M. E. & Collins, P. L. ( 1998; ). The NS1 protein of human respiratory syncytial virus is a potent inhibitor of minigenome transcription and RNA replication. J Virol 72, 1452–1461.
    [Google Scholar]
  2. Bailey, D., Chard, L. S., Dash, P., Barrett, T. & Banyard, A. C. ( 2007; ). Reverse genetics for peste-des-petits-ruminants virus (PPRV): promoter and protein specificities. Virus Res 126, 250–255.[CrossRef]
    [Google Scholar]
  3. Bankamp, B., Kearney, S. P., Liu, X., Bellini, W. J. & Rota, P. A. ( 2002; ). Activity of polymerase proteins of vaccine and wild-type measles virus strains in a minigenome replication assay. J Virol 76, 7073–7081.[CrossRef]
    [Google Scholar]
  4. Bankamp, B., Wilson, J., Bellini, W. J. & Rota, P. A. ( 2005; ). Identification of naturally occurring amino acid variations that affect the ability of the measles virus C protein to regulate genome replication and transcription. Virology 336, 120–129.[CrossRef]
    [Google Scholar]
  5. Baron, M. D. & Barrett, T. ( 2000; ). Rinderpest viruses lacking the C and V proteins show specific defects in growth and transcription of viral RNAs. J Virol 74, 2603–2611.[CrossRef]
    [Google Scholar]
  6. Baron, M. D., Shaila, M. S. & Barrett, T. ( 1993; ). Cloning and sequence analysis of the phosphoprotein gene of rinderpest virus. J Gen Virol 74, 299–304.[CrossRef]
    [Google Scholar]
  7. Brown, D. D., Rima, B. K., Allen, I. V., Baron, M. D., Banyard, A. C., Barret, T. & Duprex, W. P. ( 2005; ). Rational attenuation of a morbillivirus by modulating the activity of the RNA-dependent RNA polymerase.. J Virol 79, 14330–14338.[CrossRef]
    [Google Scholar]
  8. Buchholz, U. J., Finke, S. & Conzelmann, K.-K. ( 1999; ). Generation of bovine respiratory syncytial virus (BRSV) from cDNA: BRSV NS2 is not essential for virus replication in tissue culture, and the human RSV leader region acts as a functional BRSV genome promoter. J Virol 73, 251–259.
    [Google Scholar]
  9. Chua, K. B., Bellini, W. J., Rota, P. A., Harcourt, B. H., Tamin, A., Lam, S. K., Ksiazek, T. G., Rollin, P. E., Zaki, S. R. & other authors ( 2000; ). Nipah virus: a recently emergent deadly paramyxovirus. Science 288, 1432–1435.[CrossRef]
    [Google Scholar]
  10. Curran, J., Boeck, R. & Kolakofsky, D. ( 1991; ). The Sendai virus P gene expresses both an essential protein and an inhibitor of RNA synthesis by shuffling modules via mRNA editing. EMBO J 10, 3079–3085.
    [Google Scholar]
  11. Curran, J., Marq, J. B. & Kolakofsky, D. ( 1992; ). The Sendai virus nonstructural C proteins specifically inhibit viral mRNA synthesis. Virology 189, 647–656.[CrossRef]
    [Google Scholar]
  12. Didcock, L., Young, D. F., Goodbourn, S. & Randall, R. E. ( 1999; ). The V protein of simian virus 5 inhibits interferon signaling by targeting STAT1 for proteasome-mediated degradation. J Virol 73, 9928–9933.
    [Google Scholar]
  13. Durbin, A. P., Hall, S. L., Siew, J. W., Whitehead, S. S., Collins, P. L. & Murphy, B. R. ( 1997; ). Recovery of infectious human parainfluenza virus type 3 from cDNA. Virology 235, 323–332.[CrossRef]
    [Google Scholar]
  14. Galinski, M. S., Troy, R. M. & Banerjee, A. K. ( 1992; ). RNA editing in the phosphoprotein gene of the human parainfluenza virus type 3. Virology 186, 543–550.[CrossRef]
    [Google Scholar]
  15. Goodbourn, S., Didcock, L. & Randall, R. E. ( 2000; ). Interferons: cell signaling, immune modulation, antiviral response and virus countermeasures. J Gen Virol 81, 2341–2364.
    [Google Scholar]
  16. Grogan, C. C. & Moyer, S. A. ( 2001; ). Sendai virus wild-type and mutant C proteins show a direct correlation between L polymerase binding and inhibition of viral RNA synthesis. Virology 288, 96–108.[CrossRef]
    [Google Scholar]
  17. Halpin, K., Bankamp, B., Harcourt, B. H., Bellini, W. J. & Rota, P. A. ( 2004; ). Nipah virus conforms to the rule of six in a minigenome replication assay. J Gen Virol 85, 701–707.[CrossRef]
    [Google Scholar]
  18. Harcourt, B. H., Tamin, A., Ksiazek, T. G., Rollin, P. E., Anderson, L. J., Bellini, W. J. & Rota, P. A. ( 2000; ). Molecular characterization of Nipah virus, a newly emergent paramyxovirus. Virology 271, 334–349.[CrossRef]
    [Google Scholar]
  19. Harcourt, B. H., Tamin, A., Halpin, K., Ksiazek, T. G., Rollin, P. E., Bellini, W. J. & Rota, P. A. ( 2001; ). Molecular characterization of the polymerase gene and genomic termini of Nipah virus. Virology 287, 192–201.[CrossRef]
    [Google Scholar]
  20. He, B., Paterson, G., Stock, N., Durbin, J. E., Durbin, R. K., Goodbourn, S., Randall, R. E. & Lamb, R. A. ( 2002; ). Recovery of paramyxovirus simian virus 5 with a V protein lacking the conserved cysteine-rich domain: the multifunctional V protein blocks both interferon-β induction and interferon signaling. Virology 303, 15–32.[CrossRef]
    [Google Scholar]
  21. Horikami, S. M., Smallwood, S. & Moyer, S. A. ( 1996; ). The Sendai virus V protein interacts with the NP protein to regulate viral genome RNA replication. Virology 222, 383–390.[CrossRef]
    [Google Scholar]
  22. Hsu, V. P., Hossain, M. J., Parashar, U. D., Ali, M. M., Ksiazek, T. G., Kuzmin, I., Niezgoda, M., Rupprecht, C., Bresee, J. & Breiman, R. F. ( 2004; ). Nipah virus reemergence, Bangadesh. Emerg Infect Dis 10, 2082–2087.[CrossRef]
    [Google Scholar]
  23. Hummel, K. B., Lowe, L., Bellini, W. J. & Rota, P. A. ( 2006; ). Development of quantitative gene-specific real-time RT-PCR assays for the detection of measles virus in clinical specimens. J Virol Methods 132, 166–173.[CrossRef]
    [Google Scholar]
  24. Jeyaseelan, K., Ma, D. & Armugam, A. ( 2001; ). Real-time detection of gene promoter activity: quantitation of toxin gene transcription. Nucleic Acids Res 29, E58 [CrossRef]
    [Google Scholar]
  25. Kato, A., Kiyotani, K., Sakai, Y., Yoshida, T. & Nagai, Y. ( 1997a; ). The paramyxovirus, Sendai virus, V protein encodes a luxury function required for viral pathogenesis. EMBO J 16, 578–587.[CrossRef]
    [Google Scholar]
  26. Kato, A., Kiyotani, K., Sakai, Y., Yoshida, T., Shioda, T. & Nagai, Y. ( 1997b; ). Importance of the cysteine-rich carboxyl-terminal half of V protein for Sendai virus pathogenesis. J Virol 71, 7266–7272.
    [Google Scholar]
  27. Kato, A., Ohnishi, Y., Hishiyama, M., Kohase, M., Saito, S., Tashiro, M. & Nagai, Y. ( 2002; ). The amino-terminal half of Sendai virus C protein is not responsible for either counteracting the antiviral action of interferons or down-regulating viral RNA synthesis. J Virol 76, 7114–7124.[CrossRef]
    [Google Scholar]
  28. Kato, A., Cortese-Grogan, C., Moyer, S. A., Sugahara, F., Sakaguchi, T., Kubota, T., Otsuki, N., Kohase, M., Tashiro, M. & Nagai, Y. ( 2004; ). Characterization of the amino acid residues of Sendai virus C protein that are critically involved in its interferon antagonism and RNA synthesis down-regulation. J Virol 78, 7443–7454.[CrossRef]
    [Google Scholar]
  29. Komatsu, T., Takeuchi, K., Yokoo, J. & Gotoh, B. ( 2004; ). C and V proteins of Sendai virus target signaling pathways leading to IRF-3 activation for the negative regulation of interferon-beta production. Virology 325, 137–148.[CrossRef]
    [Google Scholar]
  30. Lamb, R. A. & Kolakofsky, D. ( 2001; ). Paramyxoviridae: the viruses and their replication. In Fields Virology, 4th edn, pp. 1305–1340. Edited by D. M. Knipe & P. M. Howley. Philadelphia, PA: Lippincott Williams & Wilkins.
  31. Lin, Y., Horvath, F., Aligo, J. A., Wilson, R. & He, B. ( 2005; ). The role of simian virus 5 V protein on viral RNA synthesis. Virology 338, 270–280.[CrossRef]
    [Google Scholar]
  32. Luby, S. P., Rahman, M., Hossain, M. J., Blum, L. S., Husain, M. M., Gurley, E., Khan, R., Ahmed, B.-N. & Rahman, S. ( 2006; ). Foodborne transmission of Nipah virus, Bangladesh. Emerg Infect Dis 12, 1888–1894.[CrossRef]
    [Google Scholar]
  33. Magoffin, D. E., Mackenzie, J. S. & Wang, L.-F. ( 2007; ). Genetic analysis of J-virus and Beilong virus using minireplicons. Virology 364, 103–111.[CrossRef]
    [Google Scholar]
  34. Malur, A. G., Hoffman, M. A. & Banerjee, A. K. ( 2004; ). The human parainfluenza virus type 3 (HPIV 3) C protein inhibits viral transcription. Virus Res 99, 199–204.[CrossRef]
    [Google Scholar]
  35. Matsuoka, Y., Curran, J., Pelet, T., Kolakofsky, D., Ray, R. & Compans, R. W. ( 1991; ). The P gene of human parainfluenza virus type 1 encodes P and C proteins, but not a cysteine-rich V protein. J Virol 65, 3406–3410.
    [Google Scholar]
  36. Mayo, M. A. ( 2002; ). A summary of taxonomic changes recently approved by ICTV. Arch Virol 147, 1655–1656.[CrossRef]
    [Google Scholar]
  37. Nanda, S. K. & Baron, M. D. ( 2006; ). Rinderpest virus blocks type I and type II interferon action: role of structural and nonstructural proteins. J Virol 80, 7555–7568.[CrossRef]
    [Google Scholar]
  38. Ohno, S., Ono, N., Takeda, M., Takeuchi, K. & Yanagi, Y. ( 2004; ). Dissection of measles virus V protein in relation to its ability to block alpha/beta interferon signal transduction. J Gen Virol 85, 2991–2999.[CrossRef]
    [Google Scholar]
  39. Park, M. S., Shaw, M. L., Muñoz-Jordan, J., Cros, J. F., Nakaya, T., Bouvier, N., Palese, P., García-Sastre, A. & Basler, C. F. ( 2003; ). Newcastle disease virus (NDV)-based assay demonstrates inerferon-antagonist activity for the NDV V protein and the Nipah virus V, W, and C proteins. J Virol 77, 1501–1511.[CrossRef]
    [Google Scholar]
  40. Parks, C. L., Witko, S. E., Kotash, C., Lin, S. L., Sidhu, M. S. & Udem, S. A. ( 2006; ). Role of V protein RNA binding in inhibition of measles virus minigenome replication. Virology 348, 96–106.[CrossRef]
    [Google Scholar]
  41. Patterson, J. B., Thomas, D., Lewicki, H., Billeter, M. A. & Oldstone, M. B. ( 2000; ). V and C proteins of measles virus function as virulence factors in vivo. Virology 267, 80–89.[CrossRef]
    [Google Scholar]
  42. Poole, E., He, B., Lamb, R. A., Randall, R. E. & Goodbourn, S. ( 2002; ). The V proteins of simian virus 5 and other paramyxoviruses inhibit induction of interferon-β. Virology 303, 33–46.[CrossRef]
    [Google Scholar]
  43. Reutter, G. L., Cortese-Grogan, C., Wilson, J. & Moyer, S. A. ( 2001; ). Mutations in the measles virus C protein that up regulate viral RNA synthesis. Virology 285, 100–109.[CrossRef]
    [Google Scholar]
  44. Rodriguez, J. J., Parisien, J.-P. & Horvath, C. M. ( 2002; ). Nipah virus V protein evades alpha and gamma interferons by preventing STAT1 and STAT2 activation and nuclear accumulation. J Virol 76, 11476–11483.[CrossRef]
    [Google Scholar]
  45. Sambrook, J. & Russell, D. W. ( 2001; ). Molecular Cloning: a Laboratory Manual, 3rd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press.
  46. Shaffer, J. A., Bellini, W. J. & Rota, P. A. ( 2003; ). The C protein of measles virus inhibits the type I interferon response. Virology 315, 389–397.[CrossRef]
    [Google Scholar]
  47. Sidhu, M. S., Chan, J., Kaelin, K., Spielhofer, P., Radecke, F., Schneider, H., Masurekar, M., Dowling, P. C., Billeter, M. A. & Udem, S. A. ( 1995; ). Rescue of synthetic measles virus minireplicons: measles genomic termini direct efficient expression and propagation of a reporter gene. Virology 208, 800–807.[CrossRef]
    [Google Scholar]
  48. Smallwood, S. & Moyer, S. A. ( 2004; ). The L polymerase protein of parainfluenza virus 3 forms an oligomer and can interact with the heterologous Sendai virus L, P and C proteins. Virology 318, 439–450.[CrossRef]
    [Google Scholar]
  49. Svenda, M., Hjertner, B., Linne, T. & Berg, M. ( 2002; ). Both the P and V proteins of the porcine rubulavirus LPMV interact with the NP protein via their respective C-terminal unique parts. Virus Res 83, 31–41.[CrossRef]
    [Google Scholar]
  50. Sweetman, D. A., Miskin, J. & Baron, M. D. ( 2001; ). Rinderpest virus C and V proteins interact with the major (L) component of the viral polymerase. Virology 281, 193–204.[CrossRef]
    [Google Scholar]
  51. Tapparel, C., Hausmann, S., Pelet, T., Curran, J., Kolakofsky, D. & Roux, L. ( 1997; ). Inhibition of Sendai virus genome replication due to promoter-increased selectivity: a possible role for the accessory C proteins. J Virol 71, 9588–9599.
    [Google Scholar]
  52. Tober, C., Seufert, M., Schneider, H., Billeter, M. A., Johnston, I. C. D., Niewiesk, S., ter Meulen, V. & Schneider-Schaulies, S. ( 1998; ). Expression of measles virus V protein is associated with pathogenicity and control of viral RNA synthesis. J Virol 72, 8124–8132.
    [Google Scholar]
  53. Witko, S. E., Kotash, C., Sidhu, M. S., Udem, S. A. & Parks, C. L. ( 2006; ). Inhibition of measles virus minireplicon-encoded reporter gene expression by V protein. Virology 348, 107–119.[CrossRef]
    [Google Scholar]
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