1887

Abstract

Variation in PrP prion gene sequence appears to modulate susceptibility to chronic wasting disease (CWD), a naturally occurring prion disease affecting four North American species of the family Cervidae. Wapiti () PrP is polymorphic at codon 132 [methionine (M) or leucine (L)]. We genotyped 171 samples, collected between 2002 and 2005 from CWD-infected and uninfected wapiti from three free-ranging populations in Colorado, USA, to study influences of PrP polymorphisms on CWD susceptibility further. Overall genotype frequencies for 124 apparently uninfected animals were 65.3 % MM, 32.3 % ML and 2.4 % LL; for 47 CWD-infected animals, these frequencies were 70.2 % MM, 27.7 % ML and 2.1 % LL. Surprisingly, our data revealed that, among recent (approx. 2002–2005) CWD cases detected in free-ranging Colorado wapiti, the three PrP codon 132 genotypes were represented in proportion to their abundance in sampled populations (≥0.24) and all three genotypes showed equivalent susceptibility to infection.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/vir.0.83424-0
2008-05-01
2020-09-24
Loading full text...

Full text loading...

/deliver/fulltext/jgv/89/5/1324.html?itemId=/content/journal/jgv/10.1099/vir.0.83424-0&mimeType=html&fmt=ahah

References

  1. Baeten L. A., Powers B. E., Jewell J. E., Spraker T. R., Miller M. W. 2007; A natural case of chronic wasting disease in a free-ranging moose ( Alces alces shirasi ). J Wildl Dis 43:309–314 [CrossRef]
    [Google Scholar]
  2. Balachandran A., Spraker T. R., O'Rourke K. I., Williams E. S., McLane J. 2002; Sub-clinical chronic wasting disease (CWD) in farmed Rocky Mountain elk in Canada: histopathological, immunohistochemical (IHC) and PrP genotyping findings in the “source” farm. In International Conference on Transmissible Spongiform Encephalopathies Edinburgh: 15–18 September 2002, abstract P1.18
    [Google Scholar]
  3. Baylis M., Goldmann W. 2004; The genetics of scrapie in sheep and goats. Curr Mol Med 4:385–396 [CrossRef]
    [Google Scholar]
  4. Browning S. R., Mason G. L., Seward T., Green M., Eliason G. A., Mathiason C., Miller M. W., Williams E. S., Hoover E., Telling G. C. 2004; Transmission of prions from mule deer and elk with chronic wasting disease to transgenic mice expressing cervid PrP. J Virol 78:13345–13350 [CrossRef]
    [Google Scholar]
  5. Bruce M. E. 1993; Scrapie strain variation and mutation. Br Med Bull 49:822–838
    [Google Scholar]
  6. Conner M. M., Miller M. W. 2004; Movement patterns and spatial epidemiology of a prion disease in mule deer population units. Ecol Appl 14:1870–1881 [CrossRef]
    [Google Scholar]
  7. Hamir A. N., Gidlewski T., Spraker T. R., Miller J. M., Creekmore L., Crocheck M., Cline T., O'Rourke K. I. 2006; Preliminary observations of genetic susceptibility of elk ( Cervus elaphus nelsoni ) to chronic wasting disease by experimental oral inoculation. J Vet Diagn Invest 18:110–114 [CrossRef]
    [Google Scholar]
  8. Heaton M. P., Leymaster K. A., Freking B. A., Hawk D. A., Smith T. P. L., Keele J. W., Snelling W. M., Fox J. M., Chitko-McKown C. G., Laegreid W. W. 2003; Prion gene sequence variation within diverse groups of U.S. sheep, beef cattle, and deer. Mamm Genome 14:765–777 [CrossRef]
    [Google Scholar]
  9. Hibler C. P., Wilson K. L., Spraker T. R., Miller M. W., Zink R. R., DeBuse L. L., Andersen E., Schweitzer D., Kennedy J. A. other authors 2003; Field validation and assessment of an enzyme-linked immunosorbent assay for detecting chronic wasting disease in mule deer ( Odocoileus hemionus ), white-tailed deer ( Odocoileus virginianus ), and Rocky Mountain elk ( Cervus elaphus nelsoni ). J Vet Diagn Invest 15:311–319 [CrossRef]
    [Google Scholar]
  10. Huson H. J., Happ G. M. 2006; Polymorphisms of the prion protein gene (PRNP) in Alaskan moose ( Alces alces gigas ). Anim Genet 37:425–426 [CrossRef]
    [Google Scholar]
  11. Jewell J. E., Conner M. M., Wolfe L. L., Miller M. W., Williams E. S. 2005; Low frequency of PrP genotype 225SF among free-ranging mule deer ( Odocoileus hemionus ) with chronic wasting disease. J Gen Virol 86:2127–2134 [CrossRef]
    [Google Scholar]
  12. Johnson C., Johnson J., Clayton M., McKenzie D., Aiken J. 2003; Prion protein gene heterogeneity in free-ranging white-tailed deer within the chronic wasting disease affected region of Wisconsin. J Wildl Dis 39:576–581 [CrossRef]
    [Google Scholar]
  13. Mead S. 2006; Prion disease genetics. Eur J Hum Genet 14:273–281 [CrossRef]
    [Google Scholar]
  14. Miller M. W., Conner M. M. 2005; Epidemiology of chronic wasting disease in free-ranging mule deer: spatial, temporal, and demographic influences on observed prevalence patterns. J Wildl Dis 41:275–290 [CrossRef]
    [Google Scholar]
  15. Miller M. W., Williams E. S., McCarty C. W., Spraker T. R., Kreeger T. J., Larsen C. T., Thorne E. T. 2000; Epizootiology of chronic wasting disease in free-ranging cervids in Colorado and Wyoming. J Wildl Dis 36:676–690 [CrossRef]
    [Google Scholar]
  16. O'Rourke K. I., Besser T. E., Miller M. W., Cline T. F., Spraker T. R., Jenny A. L., Wild M. A., Zebarth G. L., Williams E. S. 1999; PrP genotypes of captive and free-ranging Rocky Mountain elk ( Cervus elaphus nelsoni ) with chronic wasting disease. J Gen Virol 80:2765–2769
    [Google Scholar]
  17. O'Rourke K. I., Spraker T. R., Zhuang D., Greenlee J. J., Gidlewski T. E., Hamir A. N. 2007; Elk with a long incubation prion disease phenotype have a unique PrPd profile. Cell Mol Dev Neurosc 18:1935–1938
    [Google Scholar]
  18. Race R. E., Raines A., Baron T. G. M., Miller M. W., Allen J., Williams E. S. 2002; Comparison of abnormal prion protein glycoform patterns from transmissible spongiform encephalopathy agent-infected deer, elk, sheep, and cattle. J Virol 76:12365–12368 [CrossRef]
    [Google Scholar]
  19. Raymond G. J., Raymond L. D., Meade-White K. D., Hughson A. G., Favara C., Gardner D., Williams E. S., Miller M. W., Race R. E., Caughey B. 2007; Transmission and adaptation of chronic wasting disease to hamster and transgenic mice: evidence for strains. J Virol 81:4305–4314 [CrossRef]
    [Google Scholar]
  20. Spraker T. R., Miller M. W., Williams E. S., Getzy D. M., Adrian W. J., Schoonveld G. G., Spowart R. A., O'Rourke K. I., Miller J. M., Merz P. A. 1997; Spongiform encephalopathy in free-ranging mule deer ( Odocoileus hemionus ), white-tailed deer ( Odocoileus virginianus ) and Rocky Mountain elk ( Cervus elaphus nelsoni ) in northcentral Colorado. J Wildl Dis 33:1–6 [CrossRef]
    [Google Scholar]
  21. Spraker T. R., Balachandran A., Zhuang D., O'Rourke K. I. 2004; Variable patterns of distribution of PrPCWD in the obex and cranial lymphoid tissues of Rocky Mountain elk ( Cervus elaphus nelsoni) with subclinical chronic wasting disease. Vet Rec 155:295–302 [CrossRef]
    [Google Scholar]
  22. Tamguney G., Giles K., Bouzamondo-Bernstein E., Bosque P. J., Miller M. W., Safar J., DeArmond S. J., Prusiner S. B. 2006; Transmission of elk and deer prions to transgenic mice. J Virol 80:9104–9114 [CrossRef]
    [Google Scholar]
  23. Williams E. S. 2005; Chronic wasting disease. Vet Pathol 42:530–549 [CrossRef]
    [Google Scholar]
  24. Williams E. S., Young S. 1980; Chronic wasting disease of captive mule deer: a spongiform encephalopathy. J Wildl Dis 16:89–98 [CrossRef]
    [Google Scholar]
  25. Williams E. S., Young S. 1982; Spongiform encephalopathy of Rocky Mountain elk. J Wildl Dis 18:465–471 [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/vir.0.83424-0
Loading
/content/journal/jgv/10.1099/vir.0.83424-0
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error