1887

Abstract

Virus-resistant transgenic plants have been created primarily through the expression of viral sequences. It has been hypothesized that recombination between the viral transgene mRNA and the RNA of an infecting virus could generate novel viruses. As mRNA/viral RNA recombination can occur in virus-resistant transgenic plants, the key to testing this risk hypothesis is to compare the populations of recombinant viruses generated in transgenic and non-transgenic plants. This has been done with two cucumoviral systems, involving either two strains of cucumber mosaic virus (CMV), or CMV and the related tomato aspermy virus (TAV). Although the distribution of the sites of recombination in the CMV/CMV and TAV/CMV systems was quite different, equivalent populations of recombinant viruses were observed in both cases. These results constitute the first comparison of the populations of recombinants in transgenic and non-transgenic plants, and suggest that there is little risk of emergence of recombinant viruses in these plants, other than those that could emerge in non-transgenic plants.

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2008-01-01
2019-10-22
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References

  1. Aaziz, R. & Tepfer, M. ( 1999; ). Recombination between genomic RNAs of two cucumoviruses under conditions of minimal selection pressure. Virology 263, 282–289.[CrossRef]
    [Google Scholar]
  2. Abel, P. P., Nelson, R. S., De, B., Hoffmann, N., Rogers, S. G., Fraley, R. T. & Beachy, R. N. ( 1986; ). Delay of disease development in transgenic plants that express the tobacco mosaic virus coat protein gene. Science 232, 738–743.[CrossRef]
    [Google Scholar]
  3. Ali, A., Li, H., Schneider, W. L., Sherman, D. J., Gray, S., Smith, D. & Roossinck, M. J. ( 2006; ). Analysis of genetic bottlenecks during horizontal transmission of cucumber mosaic virus. J Virol 80, 8345–8350.[CrossRef]
    [Google Scholar]
  4. Carrère, I., Tepfer, M. & Jacquemond, M. ( 1999; ). Recombinants of cucumber mosaic virus (CMV): determinants of host range and symptomatology. Arch Virol 144, 365–379.[CrossRef]
    [Google Scholar]
  5. Chen, Y. K., Goldbach, R. & Prins, M. ( 2002; ). Inter- and intramolecular recombinations in the cucumber mosaic virus genome related to adaptation to alstroemeria. J Virol 76, 4119–4124.[CrossRef]
    [Google Scholar]
  6. de Wispelaere, M., Gaubert, S., Trouilloud, S., Belin, C. & Tepfer, M. ( 2005; ). A map of the diversity of RNA3 recombinants appearing in plants infected with cucumber mosaic virus and tomato aspermy virus. Virology 331, 117–127.[CrossRef]
    [Google Scholar]
  7. Devergne, J. C., Cardin, L. & Marais, A. ( 1969; ). Etude d'une souche du virus de la mosaïque du concombre isolée en France de Ranunculus asiaticus L. Ann Phytopathol 1, 329–337 (in French).
    [Google Scholar]
  8. Dietrich, C., Miller, J., McKenzie, G., Palkovics, L., Balázs, E., Palukaitis, P. & Maiss, E. ( 2007; ). No recombination detected in artificial potyvirus mixed infections and between potyvirus derived transgenes and heterologous challenging potyviruses. Environ Biosafety Res 6, 207–218.[CrossRef]
    [Google Scholar]
  9. Fernandez-Delmond, I., Pierrugues, O., de Wispelaere, M., Guilbaud, L., Gaubert, S., Divéki, Z., Godon, C., Tepfer, M. & Jacquemond, M. ( 2004; ). A novel strategy for creating recombinant infectious RNA virus genomes. J Virol Methods 121, 247–257.[CrossRef]
    [Google Scholar]
  10. Goldbach, R., Bucher, E. & Prins, M. ( 2003; ). Resistance mechanisms to plant viruses: an overview. Virus Res 92, 207–212.[CrossRef]
    [Google Scholar]
  11. Hwang, C. K., Svarovskaia, E. S. & Pathak, V. K. ( 2001; ). Dynamic copy choice: steady state between murine leukemia virus polymerase and polymerase-dependent RNase H activity determines frequency of in vivo template switching. Proc Natl Acad Sci U S A 98, 12209–12214.[CrossRef]
    [Google Scholar]
  12. Jacquemond, M. & Lot, H. ( 1981; ). L'ARN satellite du virus de la mosaïque du concombre. I. Comparaison de l'aptitude à induire la nécrose létale de la tomate d'ARN satellites isolés de plusieurs souches de virus. Agronomie 1, 927–932 (in French).[CrossRef]
    [Google Scholar]
  13. Jacquemond, M., Teycheney, P.-Y., Carrère, I., Navas-Castillo, J. & Tepfer, M. ( 2001; ). Resistance phenotypes of transgenic tobacco plants expressing different cucumber mosaic virus (CMV) coat protein genes. Mol Breed 8, 85–94.[CrossRef]
    [Google Scholar]
  14. Jakab, G., Vaistij, F. E., Droz, E. & Malnoë, P. ( 1997; ). Transgenic plants expressing viral sequences create a favourable environment for recombination between viral sequences. In Virus-Resistant Transgenic Plants: Potential Ecological Impact, pp. 45–51. Edited by M. Tepfer & E. Balazs. Heidelberg: Springer.
  15. Koenig, R. & Buttner, G. ( 2004; ). Strategies for the detection of potential beet necrotic yellow vein virus genome recombinations which might arise as a result of growing A type coat protein gene-expressing sugarbeets in soil containing B type virus. Transgenic Res 13, 21–28.[CrossRef]
    [Google Scholar]
  16. Li, H. & Roossinck, M. J. ( 2004; ). Genetic bottlenecks reduce population variation in an experimental RNA virus population. J Virol 78, 10582–10587.[CrossRef]
    [Google Scholar]
  17. Maniatis, T., Fritsch, E. F. & Sambrook, J. ( 1982; ). Molecular Cloning: a Laboratory Manual. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory.
  18. Meier, M. & Truve, E. ( 2006; ). An attempt to identify recombinants between two sobemoviruses in doubly infected oat plants. Environ Biosafety Res 5, 47–56.[CrossRef]
    [Google Scholar]
  19. Moreno, I. M., Bernal, J. J., Garcia de Blas, B., Rodriguez-Cerezo, E. & Garcia-Arenal, F. ( 1997; ). The expression level of the 3a movement protein determines differences in severity of symptoms between two strains of tomato aspermy cucumovirus. Mol Plant Microbe Interact 10, 171–179.[CrossRef]
    [Google Scholar]
  20. Pierrugues, O., Guilbaud, L., Fernandez-Delmond, I., Fabre, F., Tepfer, M. & Jacquemond, M. ( 2007; ). Biological properties and relative fitness of inter-subgroup cucumber mosaic virus RNA 3 recombinants produced in vitro. J Gen Virol 88, 2852–2861.[CrossRef]
    [Google Scholar]
  21. Rott, M. E. & Jelkmann, W. ( 2001; ). Characterization and detection of several filamentous viruses on cherry: adaptation of an alternative cloning method (DOP-PCR), and modification of an RNA extraction protocol. Eur J Plant Pathol 107, 411–420.[CrossRef]
    [Google Scholar]
  22. Salánki, K., Balázs, E. & Burgyán, J. ( 1994; ). Nucleotide sequence and infectious in vitro transcripts of RNA 3 of tomato aspermy virus pepper isolate. Virus Res 33, 281–289.[CrossRef]
    [Google Scholar]
  23. Salánki, K., Carrère, I., Jacquemond, M., Balázs, E. & Tepfer, M. ( 1997; ). Biological properties of pseudorecombinant and recombinant strains created with cucumber mosaic virus and tomato aspermy virus. J Virol 71, 3597–3602.
    [Google Scholar]
  24. Svarovskaia, E. S., Delviks, K. A., Hwang, C. K. & Pathak, V. K. ( 2000; ). Structural determinants of murine leukemia virus reverse transcriptase that affect the frequency of template switching. J Virol 74, 7171–7178.[CrossRef]
    [Google Scholar]
  25. Tepfer, M. ( 2002; ). Risk assessment of virus-resistant transgenic plants. Annu Rev Phytopathol 40, 467–491.[CrossRef]
    [Google Scholar]
  26. Teycheney, P.-Y., Aaziz, R., Dinant, S., Salánki, K., Tourneur, C., Balázs, E., Jacquemond, M. & Tepfer, M. ( 2000; ). Synthesis of (−)-strand RNA from the 3′ untranslated region of plant viral genomes expressed in transgenic plants upon infection with related viruses. J Gen Virol 81, 1121–1126.
    [Google Scholar]
  27. Tolstrup, N., Nielsen, P. S., Kolberg, J. G., Frankel, A. M., Vissing, H. & Kauppinen, S. ( 2003; ). OligoDesign: optimal design of LNA (locked nucleic acid) oligonucleotide capture probes for gene expression profiling. Nucleic Acids Res 31, 3758–3762.[CrossRef]
    [Google Scholar]
  28. Valoczi, A., Hornyik, C., Varga, N., Burgyan, J., Kauppinen, S. & Havelda, Z. ( 2004; ). Sensitive and specific detection of microRNAs by northern blot analysis using LNA-modified oligonucleotide probes. Nucleic Acids Res 32, e175 [CrossRef]
    [Google Scholar]
  29. Wang, G., Gunic, E., Girardet, J. L. & Stoisavljevic, V. ( 1999; ). Conformationally locked nucleosides. Synthesis and hybridization properties of oligodeoxynucleotides containing 2′,4′-C-bridged 2′-deoxynucleosides. Bioorg Med Chem Lett 9, 1147–1150.[CrossRef]
    [Google Scholar]
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