1887

Abstract

The two N-terminal cleavage products, nsp1 and nsp1, of the replicase polyproteins of porcine reproductive and respiratory syndrome virus (PRRSV) each contain a papain-like autoproteinase domain, which have been named PCP and PCP, respectively. To assess their role in the PRRSV life cycle, substitutions and deletions of the presumed catalytic cysteine and histidine residues of PCP and PCP were introduced into a PRRSV infectious cDNA clone. Mutations that inactivated PCP activity completely blocked subgenomic mRNA synthesis, but did not affect genome replication. In contrast, mutants in which PCP activity was blocked proved to be non-viable and no sign of viral RNA synthesis could be detected, indicating that the correct processing of the nsp1/nsp2 cleavage site is essential for PRRSV genome replication. In conclusion, the data presented here show that a productive PRRSV life cycle depends on the correct processing of both the nsp1/nsp1 and nsp1/nsp2 junctions.

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2008-02-01
2019-11-18
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