1887

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Volume 88, Issue 12

Xeroderma pigmentosum C is involved in Epstein–Barr virus DNA replication Free

Abstract

Cellular mismatch and base-excision repair machineries have been shown to be involved in Epstein–Barr Virus (EBV) lytic DNA replication. We report here that nucleotide-excision repair (NER) may also play an important role in EBV lytic DNA replication. Firstly, the EBV BGLF4 kinase interacts with xeroderma pigmentosum C (XPC), the critical DNA damage-recognition factor of NER, in yeast and , as demonstrated by yeast two-hybrid and glutathione -transferase pull-down assays. Simultaneously, XPC was shown, by indirect immunofluorescence and co-immunoprecipitation assays, to interact and colocalize with BGLF4 in EBV-positive NA cells undergoing lytic viral replication. In addition, the efficiency of EBV DNA replication was reduced about 30–40 % by an XPC small interfering RNA. Expression of BGLF4 enhances cellular DNA-repair activity in p53-defective H1299/bcl2 cells in a host-cell reactivation assay. This enhancement was not observed in the XPC-mutant cell line XP4PA-SV unless complemented by ectopic XPC, suggesting that BGLF4 may stimulate DNA repair in an XPC-dependent manner. Overall, we suggest that the interaction of BGLF4 and XPC may be involved in DNA replication and repair and thereby enhance the efficiency of viral DNA replication.

  • Received:
  • Accepted:
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2007-12-01
2024-04-26
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Xeroderma pigmentosum C is involved in Epstein–Barr virus DNA replication
J. Gen. Virol. 88, 3234 (2007); https://doi.org/10.1099/vir.0.83212-0
/content/journal/jgv/10.1099/vir.0.83212-0
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