1887

Abstract

Reverse-genetic systems are often used to study different aspects of the viral life cycle. To date, three rescue systems have been developed for the family . These systems use T7 RNA polymerase, which is generally used in rescue systems for . In the present study, we describe a rescue system for Akabane virus (family ) that uses cDNAs and RNA polymerase I instead of T7 RNA polymerase. The utility of this system was demonstrated by the generation of a mutant with a deletion of the non-structural protein (NSs) on the S RNA segment. These results offer a new option for bunyavirus rescue.

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2007-12-01
2019-11-12
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