1887

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Volume 88, Issue 9

Adaptation of two flaviviruses results in differences in genetic heterogeneity and virus adaptability Free

Abstract

West Nile virus (WNV) is a mosquito-borne flavivirus that was first introduced into the USA in the New York City area in 1999. Since its introduction, WNV has steadily increased both its host and geographical ranges. Outbreaks of the closely related flavivirus, St. Louis encephalitis virus (SLEV), occur in the USA periodically, but levels of activity and host range are more restricted than those of WNV. Understanding the selective pressures that drive arbovirus adaptation and evolution in their disparate mosquito and avian hosts is crucial to predicting their ability to persist and re-emerge. Here, we evaluated the phenotypes of mosquito cell-adapted WNV and SLEV. Results indicated that adaptations did not translate to adaptations for either virus, yet SLEV displayed attenuated growth in both mosquitoes and chickens, while WNV generally did not. growth analyses also indicated that WNV adaptations could be generalized to cell cultures derived from other mosquito species, while SLEV could not. Analysis of genetic diversity for passaged SLEV revealed a highly homogeneous population that differed significantly from previous results of high levels of diversity in WNV. We hypothesize that this difference in genetic diversity is directly related to the viruses' success in new and changing environments in the laboratory and that differences in a viruses' ability to produce and maintain heterogeneous populations in nature may in some instances explain the variable levels of success seen among arboviruses.

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2007-09-01
2024-05-06
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Adaptation of two flaviviruses results in differences in genetic heterogeneity and virus adaptability
J. Gen. Virol. 88, 2398 (2007); https://doi.org/10.1099/vir.0.83061-0
/content/journal/jgv/10.1099/vir.0.83061-0
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