1887

Abstract

Full-length nucleotide sequences for the genome segments (S1–S6) of cytoplasmic polyhedrosis virus type 14 (HaCPV-14) have been characterized. Each segment consists of a single open reading frame with conserved motifs AGAA and AGCU at the 5′ and 3′ ends, respectively. Comparison of the proteins of HaCPV-14 with those of other members of the family suggests that S1 encodes an RNA-dependent RNA polymerase (RdRp), whilst S2 encodes a major capsid protein of the virus. Phylogenetic analysis of RdRps from 16 viruses in the family reveals that the genera and may have originated from a common insect virus ancestor. A series of viable dwarf segments originating from S5 of HaCPV-14 has been identified. Analysis of the predicted secondary structures for these dwarf segments suggests that the signals essential for replication and packaging are located within the terminal sequences of these segments.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/vir.0.82673-0
2007-03-01
2019-11-21
Loading full text...

Full text loading...

/deliver/fulltext/jgv/88/3/991.html?itemId=/content/journal/jgv/10.1099/vir.0.82673-0&mimeType=html&fmt=ahah

References

  1. Arella, M., Lavalle, C., Belloncik, S. & Furuichi, Y. ( 1988; ). Molecular cloning and characterization of cytoplasmic polyhedrosis virus polyhedrin and a viable deletion mutant gene. J Virol 62, 211–217.
    [Google Scholar]
  2. Attoui, H., Billoir, F., Cantaloube, J. F., Biagini, P., de Micco, P. & de Lamballerie, X. ( 2000a; ). Strategies for the sequence determination of viral dsRNA genomes. J Virol Methods 89, 147–158.[CrossRef]
    [Google Scholar]
  3. Attoui, H., Billoir, F., Biagini, P., de Micco, P. & de Lamballerie, X. ( 2000b; ). Complete sequence determination and genetic analysis of Banna virus and Kadipiro virus: proposal for assignment to a new genus (Seadornavirus) within the family Reoviridae. J Gen Virol 81, 1507–1515.
    [Google Scholar]
  4. Attoui, H., Mohd Jaafar, F., Belhouchet, M., Biagini, P., Cantaloube, J. F., de Micco, P. & de Lamballerie, X. ( 2005; ). Expansion of family Reoviridae to include nine-segmented dsRNA viruses: isolation and characterization of a new virus designated Aedes pseudoscutellaris reovirus assigned to a proposed genus (Dinovernavirus). Virology 343, 212–223.[CrossRef]
    [Google Scholar]
  5. Belloncik, S. ( 1989; ). Cytoplasmic polyhedrosis viruses – Reoviridae. Adv Virus Res 37, 173–209.
    [Google Scholar]
  6. Belloncik, S. ( 1996; ). Interactions of cytoplasmic polyhedrosis viruses with insects. Adv Insect Physiol 26, 235–295.
    [Google Scholar]
  7. Belloncik, S. & Mori, H. ( 1998; ). Cypoviruses. In The Insect Viruses, pp. 337–369. Edited by L. K. Miller & L. A. Ball. New York: Plenum.
  8. Gouet, P., Diprose, J. M., Grimes, J. M., Malby, R., Burroughs, J. N., Zientara, S., Stuart, D. I. & Mertens, P. P. C. ( 1999; ). The highly ordered double-stranded RNA genome of bluetongue virus revealed by crystallography. Cell 97, 481–490.[CrossRef]
    [Google Scholar]
  9. Graham, R. I., Rao, S. J., Possee, R. D., Sait, S. M., Mertens, P. P. C. & Hails, R. S. ( 2006; ). Detection and characterisation of three novel species of reovirus (Reoviridae), isolated from geographically separate populations of the winter moth Operophtera brumata (Lepidoptera: Geometridae) on Orkney. J Invertebr Pathol 91, 79–87.[CrossRef]
    [Google Scholar]
  10. Hagiwara, K. & Matsumoto, T. ( 2000; ). Nucleotide sequences of genome segments 6 and 7 of Bombyx mori cypovirus 1, encoding the viral structural proteins V4 and V5, respectively. J Gen Virol 81, 1143–1147.
    [Google Scholar]
  11. Hagiwara, K. & Naitow, H. ( 2003; ). Assembly into single-shelled virus-like particles by major capsid protein VP1 encoded by genome segment S1 of Bombyx mori cypovirus 1. J Gen Virol 84, 2439–2441.[CrossRef]
    [Google Scholar]
  12. Hagiwara, K., Tomita, M., Kobayashi, J., Miyajima, S. & Yoshimura, T. ( 1998a; ). Nucleotide sequence of Bombyx mori cytoplasmic polyhedrosis virus segment 8. Biochem Biophys Res Commun 247, 549–553.[CrossRef]
    [Google Scholar]
  13. Hagiwara, K., Tomita, M., Nakai, K., Kobayashi, J., Miyajima, S. & Yoshimura, T. ( 1998b; ). Determination of the nucleotide sequence of Bombyx mori cytoplasmic polyhedrosis virus segment 9 and its expression in BmN4 cells. J Virol 72, 5762–5768.
    [Google Scholar]
  14. Hagiwara, K., Kobayashi, J., Tomita, M. & Yoshimura, T. ( 2001; ). Nucleotide sequence of genome segment 5 from Bombyx mori cypovirus 1. Arch Virol 146, 181–187.[CrossRef]
    [Google Scholar]
  15. Hagiwara, K., Rao, S., Scott, S. W. & Carner, G. R. ( 2002; ). Nucleotide sequences of segments 1, 3 and 4 of the genome of Bombyx mori cypovirus 1 encoding putative capsid proteins VP1, VP3 and VP4, respectively. J Gen Virol 83, 1477–1482.
    [Google Scholar]
  16. Hill, C. L., Booth, T. F., Prasad, B. V. V., Grimes, J. M., Mertens, P. P. C., Sutton, G. C. & Stuart, D. I. ( 1999; ). The structure of a cypovirus and the functional organization of dsRNA viruses. Nat Struct Biol 6, 565–568.[CrossRef]
    [Google Scholar]
  17. Ikeda, K., Nagaoka, S., Winkler, S., Kotani, K., Yagi, H., Nakanishi, K., Miyajima, S., Kobayashi, J. & Mori, H. ( 2001; ). Molecular characterization of Bombyx mori cytoplasmic polyhedrosis virus genome segment 4. J Virol 75, 988–995.[CrossRef]
    [Google Scholar]
  18. Lambden, P. R., Cooke, S. J., Caul, E. O. & Clarke, I. N. ( 1992; ). Cloning of noncultivatable human rotavirus by single primer amplification. J Virol 66, 1817–1822.
    [Google Scholar]
  19. Li, Y., Tan, L., Li, Y., Chen, W., Zhang, J. & Hu, Y. ( 2006; ). Identification and genome characterization of Heliothis armigera cypovirus types 5 and 14 and Heliothis assulta cypovirus type 14. J Gen Virol 87, 387–394.[CrossRef]
    [Google Scholar]
  20. Mertens, P. P. C., Crook, N. E., Rubinstein, R., Pedley, S. & Payne, C. C. ( 1989; ). Cytoplasmic polyhedrosis virus classification by electropherotype; validation by serological analyses and agarose gel electrophoresis. J Gen Virol 70, 173–185.[CrossRef]
    [Google Scholar]
  21. Mertens, P. P. C., Pedley, S., Crook, N. E., Rubinstein, R. & Payne, C. C. ( 1999; ). A comparison of six cypovirus isolates by cross-hybridisation of their dsRNA genome segments. Arch Virol 144, 561–576.[CrossRef]
    [Google Scholar]
  22. Mertens, P. P. C., Duncan, R., Attoui, H. & Dermody, T. S. ( 2004; ). Family Reoviridae. In Virus Taxonomy: Eighth Report of the International Committee on Taxonomy of Viruses, pp. 447–454. Edited by C. M. Fauquet, M. A. Mayo, J. Maniloff, U. Desselberger & L. A. Ball. London: Elsevier/Academic Press.
  23. Nibert, M. L. & Kim, J. ( 2004; ). Conserved sequence motifs for nucleoside triphosphate binding unique to turreted Reoviridae members and coltiviruses. J Virol 78, 5528–5530.[CrossRef]
    [Google Scholar]
  24. Nuss, D. L. & Summers, D. ( 1984; ). Variant dsRNAs associated with transmission-defective isolates of wound tumor virus represent terminally conserved remnants of genome segments. Virology 133, 276–288.[CrossRef]
    [Google Scholar]
  25. Page, R. D. M. ( 1996; ). TreeView: an application to display phylogenetic trees on personal computers. Comput Appl Biosci 12, 357–358.
    [Google Scholar]
  26. Patton, J. T. & Spencer, E. ( 2000; ). Genome replication and packaging of segmented double-stranded RNA viruses. Virology 277, 217–225.[CrossRef]
    [Google Scholar]
  27. Payne, C. C. & Rivers, C. F. ( 1976; ). A provisional classification of cytoplasmic polyhedrosis viruses based on the sizes of the RNA genome segments. J Gen Virol 33, 71–85.[CrossRef]
    [Google Scholar]
  28. Rao, S., Carner, G. R., Scott, S. W., Omura, T. & Hagiwara, K. ( 2003; ). Comparison of the amino acid sequences of RNA-dependent RNA polymerases of cypoviruses in the family Reoviridae. Arch Virol 148, 209–219.[CrossRef]
    [Google Scholar]
  29. Taniguchi, K., Kojima, K. & Urasawa, S. ( 1996; ). Nondefective rotavirus mutants with an NSP1 gene which has a deletion of 500 nucleotides, including a cysteine-rich zinc finger motif-encoding region (nucleotides 156 to 248), or which has a nonsense codon at nucleotides 153-155. J Virol 70, 4125–4130.
    [Google Scholar]
  30. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G. ( 1997; ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef]
    [Google Scholar]
  31. Tyler, K. L. & Fields, B. N. ( 1996; ). Reoviruses. In Fields Virology, 3rd edn, pp. 1597–1623. Edited by B. N. Fields, D. M. Knipe & P. M. Howley. Philadelphia, PA: Lippincott–Raven.
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/vir.0.82673-0
Loading
/content/journal/jgv/10.1099/vir.0.82673-0
Loading

Data & Media loading...

Supplements

vol. , part 3, pp. 991 – 997

Primers used for amplification of 5′ and 3′ parts of segments S1–S6 of the HaCPV genome [ PDF] (108 KB)



PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error