1887

Abstract

Swine beta interferon (swIFN-) produced in HEK 293 cells infected with a recombinant, replication-defective human adenovirus 5 (Ad5) encoding the swIFN- gene was tested for antiviral activity against (PRRSV). MARC-145 cells were incubated overnight with dilutions of supernatant fluids from HEK 293 cells infected with Ad5-swIFN- or with an Ad5 control virus (Ad5-Blue). Treated cells were infected with PRRSV; MARC-145 cells incubated with Ad5-Blue supernatants developed cytopathic effects (CPE), whereas those incubated with swIFN- showed no CPE. To confirm the antiviral activity of swIFN-, culture fluids from Ad5-swIFN--infected cells were affinity-purified on a Sepharose–anti-swIFN- matrix, and the resulting fractions exhibited antiviral activity upon infection with PRRSV. The antiviral effects were specific, as they were blocked by mAbs against swIFN-. Additional cultures of MARC-145 cells treated with swIFN--containing supernatants or affinity-purified swIFN- were infected with PRRSV and tested by real-time RT-PCR for viral RNA in culture supernatants at various times post-inoculation. These experiments confirmed the protective effects of swIFN-. swIFN- was also tested for antiviral activity on porcine alveolar macrophages (PAMs) obtained by bronchoalveolar lavage from PRRSV-negative swine. PAMs were treated with dilutions of swIFN- or Ad5-Blue culture fluids, infected with PRRSV and tested for viral RNA by real-time RT-PCR. The viral load data showed a dose-dependent protection in swIFN--treated PAMs, whereas no protection was evident from Ad5-Blue culture fluids. The data demonstrate that swIFN- protects both MARC-145 cells and PAMs from PRRSV infection.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/vir.0.82585-0
2007-03-01
2021-01-25
Loading full text...

Full text loading...

/deliver/fulltext/jgv/88/3/925.html?itemId=/content/journal/jgv/10.1099/vir.0.82585-0&mimeType=html&fmt=ahah

References

  1. Albina E., Carrat C., Charley B. 1998; Interferon-alpha response to swine arterivirus (PoAV), the porcine reproductive and respiratory syndrome virus. J Interferon Cytokine Res 18:485–490 [CrossRef]
    [Google Scholar]
  2. Batista L., Pijoan C., Dee S., Olin M., Molitor T., Joo H. S., Xiao Z., Murtaugh M. 2004; Virological and immunological responses to porcine reproductive and respiratory syndrome virus in a large population of gilts. Can J Vet Res 68:267–273
    [Google Scholar]
  3. Bautista E. M., Molitor T. 1999; IFN gamma inhibits porcine reproductive and respiratory syndrome virus replication in macrophages. Arch Virol 144:1191–1200 [CrossRef]
    [Google Scholar]
  4. Biron C. A., Sen G. C. 2001; Interferons and other cytokines. In Fields Virology , 4th edn. pp  321–351 Edited by Knipe D. M., Howley P. M. Philadelphia, PA: Lippincott Williams & Wilkins;
    [Google Scholar]
  5. Buddaert W., Van Reeth K., Pensaert M. 1998; In vivo and in vitro interferon (IFN) studies with the porcine reproductive and respiratory syndrome virus. Adv Exp Med Biol 440:461–467
    [Google Scholar]
  6. Carter Q. L., Curiel R. E. 2005; Interleukin-12 (IL-12) ameliorates the effects of porcine respiratory and reproductive syndrome virus (PRRSV) infection. Vet Immunol Immunopathol 107:105–118 [CrossRef]
    [Google Scholar]
  7. Chang H. W., Jeng C. R., Li J. J., Lin T. L., Chang C. C., Chia M. Y., Tsai Y. C., Pang V. F. 2005; Reduction of porcine reproductive respiratory syndrome virus (PRRSV) infection in swine alveolar macrophages by porcine circovirus 2 (PCV2)-induced interferon-alpha. Vet Microbiol 108:167–177 [CrossRef]
    [Google Scholar]
  8. Chinsangaram J., Moraes M. P., Koster M., Grubman M. J. 2003; Novel viral disease control strategy: adenovirus expressing alpha interferon rapidly protects swine from foot-and-mouth disease. J Virol 77:1621–1625 [CrossRef]
    [Google Scholar]
  9. Chung H. K., Lee J. H., Kim S. H., Chae C. 2004; Expression of interferon-alpha and Mx1 protein in pigs acutely infected with porcine reproductive and respiratory syndrome virus (PRRSV). J Comp Pathol 130:299–305 [CrossRef]
    [Google Scholar]
  10. Derbyshire J. B. 1989; The interferon sensitivity of selected porcine viruses. Can J Vet Res 53:52–55
    [Google Scholar]
  11. Kim H. S., Kwang J., Yoon I. J., Joo H. S., Frey M. L. 1993; Enhanced replication of porcine reproductive and respiratory syndrome (PRRS) virus in a homogenous subpopulation of MA-104 cell line. Arch Virol 133:477–483 [CrossRef]
    [Google Scholar]
  12. Lee S. M., Kleiboeker S. B. 2005; Porcine arterivirus activates the NF- κ B pathway through I κ B degradation. Virology 342:47–59 [CrossRef]
    [Google Scholar]
  13. Lee S. M., Schommer S. K., Kleibocker S. B. 2004; Porcine reproductive and respiratory syndrome virus field isolates differ in in vitro interferon phenotypes. Vet Immunol Immunopathol 102:217–231 [CrossRef]
    [Google Scholar]
  14. Lowe J. E., Husmann R., Firkins L. D., Zuckermann F. A., Goldberg T. L. 2005; Correlation of cell-mediated immunity against porcine reproductive and respiratory syndrome virus with protection against reproductive failure in sows during outbreaks of porcine reproductive and respiratory syndrome in commercial herds. J Am Vet Med Assoc 226:1707–1711 [CrossRef]
    [Google Scholar]
  15. Meier W. A., Husmann R. J., Schnitzlein W. N., Osorio F. A., Lunney J. K., Zuckermann F. A. 2004; Cytokines and synthetic double-stranded RNA augment the T helper 1 immune response of swine to porcine reproductive and respiratory syndrome virus. Vet Immunol Immunopathol 102:299–314 [CrossRef]
    [Google Scholar]
  16. Miller L. C., Laegreid W. W., Bono J. L., Chitko-McKown C. G., Fox J. M. 2004; Interferon type I in porcine reproductive and respiratory syndrome virus-infected MARC-145 cells. Arch Virol 149:2453–2463 [CrossRef]
    [Google Scholar]
  17. Moraes M. P., Mayr G. A., Grubman M. J. 2001; pAd5-Blue: direct ligation system for engineering recombinant adenovirus constructs. Biotechniques 31:1050–1056
    [Google Scholar]
  18. Murtaugh M. P., Xiao Z., Zuckermann F. 2002; Immunological responses of swine to porcine reproductive and respiratory syndrome virus infection. Viral Immunol 15:533–547 [CrossRef]
    [Google Scholar]
  19. Rossow K. D. 1998; Porcine reproductive and respiratory syndrome. Vet Pathol 35:1–20 [CrossRef]
    [Google Scholar]
  20. Rowland R. R. R., Robinson B., Stefanick J., Kim T. S., Guanghua L., Lawson S. R., Benfield D. A. 2001; Inhibition of porcine reproductive and respiratory syndrome virus by interferon-gamma and recovery of virus replication with 2-aminopurine. Arch Virol 146:539–555 [CrossRef]
    [Google Scholar]
  21. Royaee A. R., Husmann R. J., Dawson H. D., Calzada-Nova G., Schmitzlein W. M., Zuckermann F. A., Lunney J. K. 2004; Deciphering the involvement of innate immune factors in the development of the host response to PRRSV vaccination. Vet Immunol Immunopathol 102:199–216 [CrossRef]
    [Google Scholar]
  22. Van Reeth K., Labarque G., Nauwynck H., Pensaert M. 1999; Differential production of proinflammatory cytokines in the pig lung during different respiratory virus infections: correlations with pathogenicity. Res Vet Sci 67:47–52 [CrossRef]
    [Google Scholar]
  23. Wu Q., Brum M. C., Caron L., Koster M., Grubman M. J. 2003; Adenovirus-mediated type I interferon expression delays and reduces disease signs in cattle challenged with foot-and-mouth disease virus. J Interferon Cytokine Res 23:359–368 [CrossRef]
    [Google Scholar]
  24. Xiao Z., Batista L., Dee S., Halbur P., Murtaugh M. P. 2004; The level of virus-specific T-cell and macrophage recruitment in porcine reproductive and respiratory syndrome virus infection in pigs is independent of virus load. J Virol 78:5923–5933 [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/vir.0.82585-0
Loading
/content/journal/jgv/10.1099/vir.0.82585-0
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error