1887

Abstract

Maize streak disease is a severe agricultural problem in Africa and the development of maize genotypes resistant to the causal agent, (MSV), is a priority. A transgenic approach to engineering MSV-resistant maize was developed and tested in this study. A pathogen-derived resistance strategy was adopted by using targeted deletions and nucleotide-substitution mutants of the multifunctional MSV replication-associated protein gene (). Various gene constructs were tested for their efficacy in limiting replication of wild-type MSV by co-bombardment of maize suspension cells together with an infectious genomic clone of MSV and assaying replicative forms of DNA by quantitative PCR. , an MSV-sensitive grass species used as a model monocot, was then transformed with constructs that had inhibited virus replication in the transient-expression system. Challenge experiments using leafhopper-transmitted MSV indicated significant MSV resistance – from highly resistant to immune – in regenerated transgenic lines. Whereas regenerated lines containing a mutated full-length gene displayed developmental and growth defects, those containing a truncated gene both were fertile and displayed no growth defects, making the truncated gene a suitable candidate for the development of transgenic MSV-resistant maize.

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2007-01-01
2019-10-21
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