1887

Abstract

A full-length infectious cDNA clone (ic) was constructed from the genome of the dengue virus type 2 (DENV-2) Jamaica83 1409 strain, pBAC1409ic, by using a bacterial artifical chromosome plasmid system. Infectious virus was generated and characterized for growth in cell culture and for infection in mosquitoes. During construction, an isoleucine to methionine (Ile→Met) change was found at position 6 in the envelope glycoprotein sequence between low- and high-passage DENV-2 1409 strains. -transcribed genomic RNA of 1409ic with E6-Ile produced infectious virions following electroporation in mosquito cells, but not mammalian cells, while 1409ic RNA with an E6-Met mutation produced virus in both cell types. Moreover, DENV-2 1409 with the E6-Ile residue produced syncytia in C6/36 cell culture, whereas viruses with E6-Met did not. However, cell culture-derived growth-curve data and mosquito-infection rates revealed that none of the analysed DENV-2 strains differed from each other.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/vir.0.81958-0
2006-08-01
2021-10-19
Loading full text...

Full text loading...

/deliver/fulltext/jgv/87/8/2263.html?itemId=/content/journal/jgv/10.1099/vir.0.81958-0&mimeType=html&fmt=ahah

References

  1. Allison S. L., Schalich J., Stiasny K., Mandl C. W., Heinz F. X. 2001; Mutational evidence for an internal fusion peptide in flavivirus envelope protein E. J Virol 75:4268–4275 [CrossRef]
    [Google Scholar]
  2. Armstrong P. M., Rico-Hesse R. 2001; Differential susceptibility of Aedes aegypti to infection by the American and Southeast Asian genotypes of dengue type 2 virus. Vector Borne Zoonotic Dis 1:159–168 [CrossRef]
    [Google Scholar]
  3. Bennett K. E., Olson K. E., Muñoz M. de L., Fernandez-Salas I., Farfán-Ale J. A., Higgs S., Black W. C. IV, Beaty B. J. 2002; Variation in vector competence for dengue 2 virus among 24 collections of Aedes aegypti from Mexico and the United States. Am J Trop Med Hyg 67:85–92
    [Google Scholar]
  4. Blaney J. E. Jr, Hanson C. T., Hanley K. A., Murphy B. R., Whitehead S. S. 2004; Vaccine candidates derived from a novel infectious cDNA clone of an American genotype dengue virus type 2. BMC Infect Dis 4:39 [CrossRef]
    [Google Scholar]
  5. Bosio C. F., Beaty B. J., Black W. C. IV 1998; Quantitative genetics of vector competence for dengue-2 virus in Aedes aegypti . Am J Trop Med Hyg 59:965–970
    [Google Scholar]
  6. Bressanelli S., Stiasny K., Allison S. L., Stura E. A., Duquerroy S., Lescar J., Heinz F. X., Rey F. A. 2004; Structure of a flavivirus envelope glycoprotein in its low-pH-induced membrane fusion conformation. EMBO J 23:728–738 [CrossRef]
    [Google Scholar]
  7. Chambers T. J., Hahn C. S., Galler R., Rice C. M. 1990; Flavivirus genome organization, expression, and replication. Annu Rev Microbiol 44:649–688 [CrossRef]
    [Google Scholar]
  8. Cologna R., Rico-Hesse R. 2003; American genotype structures decrease dengue virus output from human monocytes and dendritic cells. J Virol 77:3929–3938 [CrossRef]
    [Google Scholar]
  9. Deubel V., Kinney R. M., Trent D. W. 1986; Nucleotide sequence and deduced amino acid sequence of the structural proteins of dengue type 2 virus, Jamaica genotype. Virology 155:365–377 [CrossRef]
    [Google Scholar]
  10. Deubel V., Kinney R. M., Trent D. W. 1988; Nucleotide sequence and deduced amino acid sequence of the nonstructural proteins of dengue type 2 virus, Jamaica genotype: comparative analysis of the full-length genome. Virology 165:234–244 [CrossRef]
    [Google Scholar]
  11. Diamond M. S., Edgil D., Roberts T. G., Lu B., Harris E. 2000a; Infection of human cells by dengue virus is modulated by different cell types and viral strains. J Virol 74:7814–7823 [CrossRef]
    [Google Scholar]
  12. Diamond M. S., Roberts T. G., Edgil D., Lu B., Ernst J., Harris E. 2000b; Modulation of dengue virus infection in human cells by alpha, beta, and gamma interferons. J Virol 74:4957–4966 [CrossRef]
    [Google Scholar]
  13. Edgil D., Diamond M. S., Holden K. L., Paranjape S. M., Harris E. 2003; Translation efficiency determines differences in cellular infection among dengue virus type 2 strains. Virology 317:275–290 [CrossRef]
    [Google Scholar]
  14. Guirakhoo F., Hunt A. R., Lewis J. G., Roehrig J. T. 1993; Selection and partial characterization of dengue 2 virus mutants that induce fusion at elevated pH. Virology 194:219–223 [CrossRef]
    [Google Scholar]
  15. Kapoor M., Zhang L., Mohan P. M., Padmanabhan R. 1995; Synthesis and characterization of an infectious dengue virus type-2 RNA genome (New Guinea C strain). Gene 162:175–180 [CrossRef]
    [Google Scholar]
  16. Kinney R. M., Butrapet S., Chang G.-J. J., Tsuchiya K. R., Roehrig J. T., Bhamarapravati N., Gubler D. J. 1997; Construction of infectious cDNA clones for dengue 2 virus: strain 16681 and its attenuated vaccine derivative, strain PDK-53. Virology 230:300–308 [CrossRef]
    [Google Scholar]
  17. Lee E., Weir R. C., Dalgarno L. 1997; Changes in the dengue virus major envelope protein on passaging and their localization on the three-dimensional structure of the protein. Virology 232:281–290 [CrossRef]
    [Google Scholar]
  18. Lewis J. A., Chang G.-J., Lanciotti R. S., Kinney R. M., Mayer L. W., Trent D. W. 1993; Phylogenetic relationships of dengue-2 viruses. Virology 197:216–224 [CrossRef]
    [Google Scholar]
  19. Loroño-Pino M. A., Farfán-Ale J. A., Zapata-Peraza A. L. 11 other authors 2004; Introduction of the American/Asian genotype of dengue 2 virus into the Yucatan State of Mexico. Am J Trop Med Hyg 71:485–492
    [Google Scholar]
  20. Mandl C. W., Ecker M., Holzmann H., Kunz C., Heinz F. X. 1997; Infectious cDNA clones of tick-borne encephalitis virus European subtype prototypic strain Neudoerfl and high virulence strain Hypr. J Gen Virol 78:1049–1057
    [Google Scholar]
  21. Modis Y., Ogata S., Clements D., Harrison S. C. 2003; A ligand-binding pocket in the dengue virus envelope glycoprotein. Proc Natl Acad Sci U S A 100:6986–6991 [CrossRef]
    [Google Scholar]
  22. Modis Y., Ogata S., Clements D., Harrison S. C. 2004; Structure of the dengue virus envelope protein after membrane fusion. Nature 427:313–319 [CrossRef]
    [Google Scholar]
  23. Monath T. P. 1994; Dengue: the risk to developed and developing countries. Proc Natl Acad Sci U S A 91:2395–2400 [CrossRef]
    [Google Scholar]
  24. Myles K. M., Pierro D. J., Olson K. E. 2004; Comparison of the transmission potential of two genetically distinct Sindbis viruses after oral infection of Aedes aegypti (Diptera: Culicidae). J Med Entomol 41:95–106 [CrossRef]
    [Google Scholar]
  25. Randolph V. B., Stollar V. 1990; Low pH-induced cell fusion in flavivirus-infected Aedes albopictus cell cultures. J Gen Virol 71:1845–1850 [CrossRef]
    [Google Scholar]
  26. Rey F. A., Heinz F. X., Mandl C., Kunz C., Harrison S. C. 1995; The envelope glycoprotein from tick-borne encephalitis virus at 2 Å resolution. Nature 375:291–298 [CrossRef]
    [Google Scholar]
  27. Rice C. M., Grakoui A., Galler R., Chambers T. J. 1989; Transcription of infectious yellow fever RNA from full-length cDNA templates produced by in vitro ligation. New Biol 1:285–296
    [Google Scholar]
  28. Rico-Hesse R. 1990; Molecular evolution and distribution of dengue viruses type 1 and 2 in nature. Virology 174:479–493 [CrossRef]
    [Google Scholar]
  29. Rico-Hesse R., Harrison L. M., Salas R. A. & 7 other authors 1997; Origins of dengue type 2 viruses associated with increased pathogenicity in the Americas. Virology 230:244–251 [CrossRef]
    [Google Scholar]
  30. Sumiyoshi H., Hoke C. H., Trent D. W. 1992; Infectious Japanese encephalitis virus RNA can be synthesized from in vitro-ligated cDNA templates. J Virol 66:5425–5431
    [Google Scholar]
  31. Summers P. L., Cohen W. H., Ruiz M. M., Hase T., Eckels K. H. 1989; Flaviviruses can mediate fusion from without in Aedes albopictus mosquito cell cultures. Virus Res 12:383–392 [CrossRef]
    [Google Scholar]
  32. Twiddy S. S., Farrar J. J., Vinh Chau N., Wills B., Gould E. A., Gritsun T., Lloyd G., Holmes E. C. 2002; Phylogenetic relationships and differential selection pressures among genotypes of dengue-2 virus. Virology 298:63–72 [CrossRef]
    [Google Scholar]
  33. Uzcategui N. Y., Camacho D., Comach G., Cuello de Uzcategui R., Holmes E. C., Gould E. A. 2001; Molecular epidemiology of dengue type 2 virus in Venezuela: evidence for in situ virus evolution and recombination. J Gen Virol 82:2945–2953
    [Google Scholar]
  34. Wang E., Ni H., Xu R., Barrett A. D. T., Watowich S. J., Gubler D. J., Weaver S. C. 2000; Evolutionary relationships of endemic/epidemic and sylvatic dengue viruses. J Virol 74:3227–3234 [CrossRef]
    [Google Scholar]
  35. Yamshchikov V. F., Wengler G., Perelygin A. A., Brinton M. A., Compans R. W. 2001; An infectious clone of the West Nile flavivirus. Virology 281:294–304 [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/vir.0.81958-0
Loading
/content/journal/jgv/10.1099/vir.0.81958-0
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error