1887

Abstract

The vaccinia virus (VACV) protein A55 is a BTB/kelch protein with a broad-complex, tramtrack and bric-a-brac (BTB) domain in the N-terminal region and five kelch repeats in the C-terminal half. The BTB/kelch subgroup of the kelch superfamily of proteins has been associated with a wide variety of functions including regulation of the cytoskeleton. VACV contains three genes predicted to encode BTB/kelch proteins: , and . The gene product has been identified as an intracellular protein of 64 kDa that is expressed late in infection. A VACV strain lacking 93.6 % of the A55R open reading frame (vΔA55) was constructed and found to have an unaltered growth rate but a different plaque morphology and cytopathic effect, as well as reduced development of VACV-induced Ca-independent cell/extracellular matrix adhesion. In a murine intradermal model of VACV infection, a virus lacking the gene induced larger lesions than wild-type and revertant control viruses.

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2006-06-01
2019-09-15
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References

  1. Adams, J., Kelso, R. & Cooley, L. ( 2000; ). The kelch repeat superfamily of proteins: propellers of cell function. Trends Cell Biol 10, 17–24.[CrossRef]
    [Google Scholar]
  2. Aguado, B., Selmes, I. P. & Smith, G. L. ( 1992; ). Nucleotide sequence of 21.8 kbp of variola major virus strain Harvey and comparison with vaccinia virus. J Gen Virol 73, 2887–2902.[CrossRef]
    [Google Scholar]
  3. Alcami, A. & Smith, G. L. ( 1992; ). A soluble receptor for interleukin-1β encoded by vaccinia virus: a novel mechanism of virus modulation of the host response to infection. Cell 71, 153–167.[CrossRef]
    [Google Scholar]
  4. Alcami, A. & Smith, G. L. ( 1995; ). Vaccinia, cowpox, and camelpox viruses encode soluble gamma interferon receptors with novel broad species specificity. J Virol 69, 4633–4639.
    [Google Scholar]
  5. Antoine, G., Scheiflinger, F., Dorner, F. & Falkner, F. G. ( 1998; ). The complete genomic sequence of the modified vaccinia Ankara strain: comparison with other orthopoxviruses. Virology 244, 365–396.[CrossRef]
    [Google Scholar]
  6. Bardwell, V. J. & Treisman, R. ( 1994; ). The POZ domain: a conserved protein–protein interaction motif. Genes Dev 8, 1664–1677.[CrossRef]
    [Google Scholar]
  7. Beattie, E., Tartaglia, J. & Paoletti, E. ( 1991; ). Vaccinia virus-encoded eIF-2 alpha homolog abrogates the antiviral effect of interferon. Virology 183, 419–422.[CrossRef]
    [Google Scholar]
  8. Bomont, P., Cavalier, L., Blondeau, F. & 10 other authors ( 2000; ). The gene encoding gigaxonin, a new member of the cytoskeletal BTB/kelch repeat family, is mutated in giant axonal neuropathy. Nat Genet 26, 370–374.[CrossRef]
    [Google Scholar]
  9. Bowie, A., Kiss-Toth, E., Symons, J. A., Smith, G. L., Dower, S. K. & O'Neill, L. A. ( 2000; ). A46R and A52R from vaccinia virus are antagonists of host IL-1 and toll-like receptor signaling. Proc Natl Acad Sci U S A 97, 10162–10167.[CrossRef]
    [Google Scholar]
  10. Brown, C. K., Turner, P. C. & Moyer, R. W. ( 1991; ). Molecular characterization of the vaccinia virus hemagglutinin gene. J Virol 65, 3598–3606.
    [Google Scholar]
  11. Chang, H. W., Watson, J. C. & Jacobs, B. L. ( 1992; ). The E3L gene of vaccinia virus encodes an inhibitor of the interferon-induced, double-stranded RNA-dependent protein kinase. Proc Natl Acad Sci U S A 89, 4825–4829.[CrossRef]
    [Google Scholar]
  12. Dobbelstein, M. & Shenk, T. ( 1996; ). Protection against apoptosis by the vaccinia virus SPI-2 (B13R) gene product. J Virol 70, 6479–6485.
    [Google Scholar]
  13. Esposito, J., Condit, R. & Obijeski, J. ( 1981; ). The preparation of orthopoxvirus DNA. J Virol Methods 2, 175–179.[CrossRef]
    [Google Scholar]
  14. Falkner, F. G. & Moss, B. ( 1990; ). Transient dominant selection of recombinant vaccinia viruses. J Virol 64, 3108–3111.
    [Google Scholar]
  15. Feierbach, B., Verde, F. & Chang, F. ( 2004; ). Regulation of a formin complex by the microtubule plus end protein tea1p. J Cell Biol 165, 697–707.[CrossRef]
    [Google Scholar]
  16. Furukawa, M., He, Y. J., Borchers, C. & Xiong, Y. ( 2003; ). Targeting of protein ubiquitination by BTB-Cullin 3-Roc1 ubiquitin ligases. Nat Cell Biol 5, 1001–1007.[CrossRef]
    [Google Scholar]
  17. Goebel, S. J., Johnson, G. P., Perkus, M. E., Davis, S. W., Winslow, J. P. & Paoletti, E. ( 1990; ). The complete DNA sequence of vaccinia virus. Virology 179, 247–266, 517–263.
    [Google Scholar]
  18. Harte, M. T., Haga, I. R., Maloney, G., Gray, P., Reading, P. C., Bartlett, N. W., Smith, G. L., Bowie, A. & O'Neill, L. A. ( 2003; ). The poxvirus protein A52R targets toll-like receptor signaling complexes to suppress host defense. J Exp Med 197, 343–351.[CrossRef]
    [Google Scholar]
  19. Heiska, L. & Carpen, O. ( 2005; ). Src phosphorylates ezrin at tyrosine 477 and induces a phosphospecific association between ezrin and a kelch-repeat protein family member. J Biol Chem 280, 10244–10252.[CrossRef]
    [Google Scholar]
  20. Hernandez, M. C., Andres-Barquin, P. J., Martinez, S., Bulfone, A., Rubenstein, J. L. & Israel, M. A. ( 1997; ). ENC-1: a novel mammalian kelch-related gene specifically expressed in the nervous system encodes an actin-binding protein. J Neurosci 17, 3038–3051.
    [Google Scholar]
  21. Horton, R. M., Hunt, H. D., Ho, S. N., Pullen, J. K. & Pease, L. R. ( 1989; ). Engineering hybrid genes without the use of restriction enzymes: gene splicing by overlap extension. Gene 77, 61–68.[CrossRef]
    [Google Scholar]
  22. Ito, N., Phillips, S. E., Yadav, K. D. & Knowles, P. F. ( 1994; ). Crystal structure of a free radical enzyme, galactose oxidase. J Mol Biol 238, 794–814.
    [Google Scholar]
  23. Jiang, S., Avraham, H. K., Park, S. Y., Kim, T. A., Bu, X., Seng, S. & Avraham, S. ( 2005; ). Process elongation of oligodendrocytes is promoted by the Kelch-related actin-binding protein Mayven. J Neurochem 92, 1191–1203.[CrossRef]
    [Google Scholar]
  24. Kang, M. I., Kobayashi, A., Wakabayashi, N., Kim, S. G. & Yamamoto, M. ( 2004; ). Scaffolding of Keap1 to the actin cytoskeleton controls the function of Nrf2 as key regulator of cytoprotective phase 2 genes. Proc Natl Acad Sci U S A 101, 2046–2051.[CrossRef]
    [Google Scholar]
  25. Kettle, S., Alcami, A., Khanna, A., Ehret, R., Jassoy, C. & Smith, G. L. ( 1997; ). Vaccinia virus serpin B13R (SPI-2) inhibits interleukin-1β-converting enzyme and protects virus-infected cells from TNF- and Fas-mediated apoptosis, but does not prevent IL-1β-induced fever. J Gen Virol 78, 677–685.
    [Google Scholar]
  26. Kochneva, G., Kolosova, I., Maksyutova, T., Ryabchikova, E. & Shchelkunov, S. ( 2005; ). Effects of deletions of kelch-like genes on cowpox virus biological properties. Arch Virol 150, 1857–1870.[CrossRef]
    [Google Scholar]
  27. Kotwal, G. J. & Moss, B. ( 1988a; ). Vaccinia virus encodes a secretory polypeptide structurally related to complement control proteins. Nature 335, 176–178.[CrossRef]
    [Google Scholar]
  28. Kotwal, G. J. & Moss, B. ( 1988b; ). Analysis of a large cluster of nonessential genes deleted from a vaccinia virus terminal transposition mutant. Virology 167, 524–537.
    [Google Scholar]
  29. Li, X., Zhang, D., Hannink, M. & Beamer, L. J. ( 2004; ). Crystal structure of the Kelch domain of human Keap1. J Biol Chem 279, 54750–54758.[CrossRef]
    [Google Scholar]
  30. Liang, X. Q., Avraham, H. K., Jiang, S. & Avraham, S. ( 2004; ). Genetic alterations of the NRP/B gene are associated with human brain tumors. Oncogene 23, 5890–5900.[CrossRef]
    [Google Scholar]
  31. Mai, A., Jung, S. K. & Yonehara, S. ( 2004; ). hDKIR, a human homologue of the Drosophila kelch protein, involved in a ring-like structure. Exp Cell Res 300, 72–83.[CrossRef]
    [Google Scholar]
  32. Mata, J. & Nurse, P. ( 1997; ). Tea1 and the microtubular cytoskeleton are important for generating global spatial order within the fisson yeast cell. Cell 89, 939–949.[CrossRef]
    [Google Scholar]
  33. McCraith, S., Holtzman, T., Moss, B. & Fields, S. ( 2000; ). Genome-wide analysis of vaccinia virus protein–protein interactions. Proc Natl Acad Sci U S A 97, 4879–4884.[CrossRef]
    [Google Scholar]
  34. McKenzie, R., Kotwal, G. J., Moss, B., Hammer, C. H. & Frank, M. M. ( 1992; ). Regulation of complement activity by vaccinia virus complement-control protein. J Infect Dis 166, 1245–1250.[CrossRef]
    [Google Scholar]
  35. Parkinson, J. E. & Smith, G. L. ( 1994; ). Vaccinia virus gene A36R encodes a M r 43–50 K protein on the surface of extracellular enveloped virus. Virology 204, 376–390.[CrossRef]
    [Google Scholar]
  36. Pintard, L., Willems, A. & Peter, M. ( 2004; ). Cullin-based ubiquitin ligases: Cul3-BTB complexes join the family. EMBO J 23, 1681–1687.[CrossRef]
    [Google Scholar]
  37. Pires de Miranda, M., Reading, P. C., Tscharke, D. C., Murphy, B. J. & Smith, G. L. ( 2003; ). The vaccinia virus kelch-like protein C2L affects calcium-independent adhesion to the extracellular matrix and inflammation in a murine intradermal model. J Gen Virol 84, 2459–2471.[CrossRef]
    [Google Scholar]
  38. Prag, S. & Adams, J. C. ( 2003; ). Molecular phylogeny of the kelch-repeat superfamily reveals an expansion of BTB/kelch proteins in animals. BMC Bioinformatics 4, 42.[CrossRef]
    [Google Scholar]
  39. Robinson, D. N. & Cooley, L. ( 1997; ). Drosophila kelch is an oligomeric ring canal actin organizer. J Cell Biol 138, 799–810.[CrossRef]
    [Google Scholar]
  40. Sanderson, C. M. & Smith, G. L. ( 1998; ). Vaccinia virus induces Ca2+-independent cell-matrix adhesion during the motile phase of infection. J Virol 72, 9924–9933.
    [Google Scholar]
  41. Sanderson, C. M., Way, M. & Smith, G. L. ( 1998; ). Virus-induced cell motility. J Virol 72, 1235–1243.
    [Google Scholar]
  42. Seet, B. T., Johnston, J. B., Brunetti, C. R. & 7 other authors ( 2003; ). Poxviruses and immune evasion. Annu Rev Immunol 21, 377–423.[CrossRef]
    [Google Scholar]
  43. Shchelkunov, S., Totmenin, A. & Kolosova, I. ( 2002; ). Species-specific differences in organization of orthopoxvirus kelch-like proteins. Virus Genes 24, 157–162.[CrossRef]
    [Google Scholar]
  44. Spence, H. J., Johnston, I., Ewart, K., Buchanan, S. J., Fitzgerald, U. & Ozanne, B. W. ( 2000; ). Krp1, a novel kelch related protein that is involved in pseudopod elongation in transformed cells. Oncogene 19, 1266–1276.[CrossRef]
    [Google Scholar]
  45. Spriggs, M. K., Hruby, D. E., Maliszewski, C. R., Pickup, D. J., Sims, J. E., Buller, R. M. & VanSlyke, J. ( 1992; ). Vaccinia and cowpox viruses encode a novel secreted interleukin-1-binding protein. Cell 71, 145–152.[CrossRef]
    [Google Scholar]
  46. Stack, J., Haga, I. R., Schroder, M., Bartlett, N. W., Maloney, G., Reading, P. C., Fitzgerald, K. A., Smith, G. L. & Bowie, A. G. ( 2005; ). Vaccinia virus protein A46R targets multiple toll-like-interleukin-1 receptor adaptors and contributes to virulence. J Exp Med 201, 1007–1018.[CrossRef]
    [Google Scholar]
  47. Tscharke, D. C. & Smith, G. L. ( 1999; ). A model for vaccinia virus pathogenesis and immunity based on intradermal injection of mouse ear pinnae. J Gen Virol 80, 2751–2755.
    [Google Scholar]
  48. Tscharke, D. C., Reading, P. C. & Smith, G. L. ( 2002; ). Dermal infection with vaccinia virus reveals roles for virus proteins not seen using other inoculation routes. J Gen Virol 83, 1977–1986.
    [Google Scholar]
  49. Tulman, E. R., Afonso, C. L., Lu, Z. & 7 other authors ( 2002; ). The genomes of sheeppox and goatpox viruses. J Virol 76, 6054–6061.[CrossRef]
    [Google Scholar]
  50. Upton, C., Mossman, K. & McFadden, G. ( 1992; ). Encoding of a homolog of the IFN-gamma receptor by myxoma virus. Science 258, 1369–1372.[CrossRef]
    [Google Scholar]
  51. Wakabayashi, N., Itoh, K., Wakabayashi, J. & 10 other authors ( 2003; ). Keap1-null mutation leads to postnatal lethality due to constitutive Nrf2 activation. Nat Genet 35, 238–245.[CrossRef]
    [Google Scholar]
  52. Wang, S., Zheng, H., Esaki, Y., Kelly, F. & Yan, W. ( 2006; ). Cullin3 is a KLHL10-interacting protein preferentially expressed during late spermiogenesis. Biol Reprod 74, 102–108.
    [Google Scholar]
  53. Wasilenko, S. T., Stewart, T. L., Meyers, A. F. & Barry, M. ( 2003; ). Vaccinia virus encodes a previously uncharacterized mitochondrial-associated inhibitor of apoptosis. Proc Natl Acad Sci U S A 100, 14345–14350.[CrossRef]
    [Google Scholar]
  54. Xue, F. & Cooley, L. ( 1993; ). Kelch encodes a component of intercellular bridges in Drosophila egg chambers. Cell 72, 681–693.[CrossRef]
    [Google Scholar]
  55. Zhang, D. D., Lo, S. C., Cross, J. V., Templeton, D. J. & Hannink, M. ( 2004; ). Keap1 is a redox-regulated substrate adaptor protein for a Cul3-dependent ubiquitin ligase complex. Mol Cell Biol 24, 10941–10953.[CrossRef]
    [Google Scholar]
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