1887

Abstract

The vaccinia virus (VACV) protein A55 is a BTB/kelch protein with a broad-complex, tramtrack and bric-a-brac (BTB) domain in the N-terminal region and five kelch repeats in the C-terminal half. The BTB/kelch subgroup of the kelch superfamily of proteins has been associated with a wide variety of functions including regulation of the cytoskeleton. VACV contains three genes predicted to encode BTB/kelch proteins: , and . The gene product has been identified as an intracellular protein of 64 kDa that is expressed late in infection. A VACV strain lacking 93.6 % of the A55R open reading frame (vΔA55) was constructed and found to have an unaltered growth rate but a different plaque morphology and cytopathic effect, as well as reduced development of VACV-induced Ca-independent cell/extracellular matrix adhesion. In a murine intradermal model of VACV infection, a virus lacking the gene induced larger lesions than wild-type and revertant control viruses.

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2006-06-01
2021-04-16
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