1887

Abstract

To evaluate the potential pathogenicity to mammals of the recent H5N1 avian , viruses recovered from dead mice infected with A/chicken/Yamaguchi/7/2004 isolated in Japan were examined. All recovered viruses from the brains of dead mice infected with this strain (without any prior adaptation to mice) had substituted the amino acid at position 627 of the PB2 protein from glutamic acid to lysine. Their mouse lethality had increased by approximately 5×10 times over that of the original virus. Histopathological analysis reinforced the finding that these variants caused more rapid and severe damage to mice than the original virus. This revealed that it might be useful to characterize the recovered virus to assess its potential pathogenicity to mammals.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/vir.0.81843-0
2006-12-01
2024-10-06
Loading full text...

Full text loading...

/deliver/fulltext/jgv/87/12/3655.html?itemId=/content/journal/jgv/10.1099/vir.0.81843-0&mimeType=html&fmt=ahah

References

  1. Chen H., Deng G., Li Z. & 7 other authors 2004; The evolution of H5N1 influenza viruses in ducks in southern China. Proc Natl Acad Sci U S A 101:10452–10457 [CrossRef]
    [Google Scholar]
  2. Chen H., Smith G. J. D., Zhang S. Y., Qin K., Wang J., Li K. S., Webster R. G., Peiris J. S. M., Guan Y. 2005; Avian flu: H5N1 virus outbreak in migratory waterfowl. Nature 436:191–192 [CrossRef]
    [Google Scholar]
  3. Fouchier R. A. M., Schneeberger P. M., Rozendaal F. W. & 11 other authors 2004; Avian influenza A virus (H7N7) associated with human conjunctivitis and a fatal case of acute respiratory distress syndrome. Proc Natl Acad Sci U S A 101:1356–1361 [CrossRef]
    [Google Scholar]
  4. Gabriel G., Dauber B., Wolff T., Planz O., Klenk H.-D., Stech J. 2005; The viral polymerase mediates adaptation of an avian influenza virus to a mammalian host. Proc Natl Acad Sci U S A 102:18590–18595 [CrossRef]
    [Google Scholar]
  5. Gao P., Watanabe S., Ito T., Goto H., Wells K., McGregor M., Cooley A. J., Kawaoka Y. 1999; Biological heterogeneity, including systemic replication in mice, of H5N1 influenza A virus isolates from humans in Hong Kong. J Virol 73:3184–3189
    [Google Scholar]
  6. Govorkova E. A., Rehg J. E., Krauss S. & 11 other authors 2005; Lethality to ferrets of H5N1 influenza viruses isolated from humans and poultry in 2004. J Virol 79:2191–2198 [CrossRef]
    [Google Scholar]
  7. Guan Y., Peiris J. S. M., Lipatov A. S., Ellis T. M., Dyrting K. C., Krauss S., Zhang L. J., Webster R. G., Shortridge K. F. 2002; Emergence of multiple genotypes of H5N1 avian influenza viruses in Hong Kong SAR. Proc Natl Acad Sci U S A 99:8950–8955 [CrossRef]
    [Google Scholar]
  8. Guan Y., Poon L. L. M., Cheung C. Y. & 10 other authors 2004; H5N1 influenza: a protean pandemic threat. Proc Natl Acad Sci U S A 101:8156–8161 [CrossRef]
    [Google Scholar]
  9. Hatta M., Gao P., Halfmann P., Kawaoka Y. 2001; Molecular basis for high virulence of Hong Kong H5N1 influenza A viruses. Science 293:1840–1842 [CrossRef]
    [Google Scholar]
  10. Keawcharoen J., Oraveerakul K., Kuiken T. & 12 other authors 2004; Avian influenza H5N1 in tigers and leopards. Emerg Infect Dis 10:2189–2191 [CrossRef]
    [Google Scholar]
  11. Li K. S., Guan Y., Wang J. & 19 other authors 2004; Genesis of a highly pathogenic and potentially pandemic H5N1 influenza virus in eastern Asia. Nature 430:209–213 [CrossRef]
    [Google Scholar]
  12. Lipatov A. S., Krauss S., Guan Y., Peiris M., Rehg J. E., Perez D. R., Webster R. G. 2003; Neurovirulence in mice of H5N1 influenza virus genotypes isolated from Hong Kong poultry in 2001. J Virol 77:3816–3823 [CrossRef]
    [Google Scholar]
  13. Liu J., Xiao H., Lei F. & 11 other authors 2005; Highly pathogenic H5N1 influenza virus infection in migratory birds. Science 309:1206 [CrossRef]
    [Google Scholar]
  14. Lu X., Tumpey T. M., Morken T., Zaki S. R., Cox N. J., Katz J. M. 1999; A mouse model for the evaluation of pathogenesis and immunity to influenza A (H5N1) viruses isolated from humans. J Virol 73:5903–5911
    [Google Scholar]
  15. Mase M., Eto M., Tanimura N., Imai K., Tsukamoto K., Horimoto T., Kawaoka Y., Yamaguchi S. 2005a; Isolation of a genotypically unique H5N1 influenza virus from duck meat imported into Japan from China. Virology 339:101–109 [CrossRef]
    [Google Scholar]
  16. Mase M., Tsukamoto K., Imada T. & 11 other authors 2005b; Characterization of H5N1 influenza A viruses isolated during the 2003–2004 influenza outbreaks in Japan. Virology 332:167–176 [CrossRef]
    [Google Scholar]
  17. Massin P., van der Werf S., Naffakh N. 2001; Residue 627 of PB2 is a determinant of cold sensitivity in RNA replication of avian influenza viruses. J Virol 75:5398–5404 [CrossRef]
    [Google Scholar]
  18. Naffakh N., Massin P., Escriou N., Crescenzo-Chaigne B., van der Werf S. 2000; Genetic analysis of the compatibility between polymerase proteins from human and avian strains of influenza A viruses. J Gen Virol 81:1283–1291
    [Google Scholar]
  19. Nishimura H., Itamura S., Iwasaki T., Kurata T., Tashiro M. 2000; Characterization of human influenza A (H5N1) virus infection in mice: neuro-, pneumo- and adipotropic infection. J Gen Virol 81:2503–2510
    [Google Scholar]
  20. Park C. H., Ishinaka M., Takada A., Kida H., Kimura T., Ochiai K., Umemura T. 2002; The invasion routes of neurovirulent A/Hong Kong/483/97 (H5N1) influenza virus into the central nervous system after respiratory infection in mice. Arch Virol 147:1425–1436 [CrossRef]
    [Google Scholar]
  21. Peiris J. S. M., Yu W. C., Leung C. W. & 9 other authors 2004; Re-emergence of fatal human influenza A subtype H5N1 disease. Lancet 363:617–619 [CrossRef]
    [Google Scholar]
  22. Puthavathana P., Auewarakul P., Charoenying P. C. & 7 other authors 2005; Molecular characterization of the complete genome of human influenza H5N1 virus isolates from Thailand. J Gen Virol 86:423–433 [CrossRef]
    [Google Scholar]
  23. Reed L. J., Muench H. 1938; A simple method of estimating fifty percent endpoints. Am J Hyg 27:493–497
    [Google Scholar]
  24. Shinya K., Hamm S., Hatta M., Ito H., Ito T., Kawaoka Y. 2004; PB2 amino acid at position 627 affects replicative efficiency, but not cell tropism, of Hong Kong H5N1 influenza A viruses in mice. Virology 320:258–266 [CrossRef]
    [Google Scholar]
  25. Shortridge K. F., Zhou N. N., Guan Y. & 12 other authors 1998; Characterization of avian H5N1 influenza viruses from poultry in Hong Kong. Virology 252:331–342 [CrossRef]
    [Google Scholar]
  26. Subbarao E. K., Kawaoka Y., Murphy B. R. 1993; Rescue of an influenza A virus wild-type PB2 gene and a mutant derivative bearing a site-specific temperature-sensitive and attenuating mutation. J Virol 67:7223–7228
    [Google Scholar]
  27. Subbarao K., Klimov A., Katz J. & 13 other authors 1998; Characterization of an avian influenza A (H5N1) virus isolated from a child with a fatal respiratory illness. Science 279:393–396 [CrossRef]
    [Google Scholar]
  28. Tanimura N., Imada T., Kashiwazaki Y., Shamusudin S., Syed Hassan S., Jamaluddin A., Russell G., White J. 2004; Reactivity of anti-Nipah virus monoclonal antibodies to formalin-fixed, paraffin-embedded lung tissues from experimental Nipah and Hendra virus infections. J Vet Med Sci 66:1263–1266 [CrossRef]
    [Google Scholar]
  29. WHO 2006; Cumulative number of confirmed human cases of avian influenza A/(H5N1) reported to WHO. http://www.who.int/csr/disease/avian_influenza/country/cases_table_2006_06_16/en/index.html
  30. Wright P. F., Webster R. G. 2001; Orthomyxoviruses. In Fields Virology , 4th edn. pp  1538–1579 Edited by Knipe D. M., Howley P. M. Philadelphia, PA: Lippincott Williams & Wilkins;
    [Google Scholar]
  31. Yuen K. Y., Chan P. K. S., Peiris M. & 8 other authors 1998; Clinical features and rapid viral diagnosis of human disease associated with avian influenza A H5N1 virus. Lancet 351:467–471 [CrossRef]
    [Google Scholar]
/content/journal/jgv/10.1099/vir.0.81843-0
Loading
/content/journal/jgv/10.1099/vir.0.81843-0
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error