1887

Abstract

The potyvirus coat protein (CP) is involved in aphid transmission, cell-to-cell movement and virus assembly, not only by binding to viral RNA, but also by self-interaction or interactions with other factors. In this study, a number of CP mutants of (SMV) containing deletions and site-directed mutations were generated and cloned into yeast two-hybrid vectors. Interaction was confirmed by the expression of reporter genes, including , and , in yeast strain AH109. Deletion of the C-terminal region of the CP caused loss of the CP–CP self-interaction ability detected in CP mutants with the C-terminal region. Alanine substitution at the amino acid positions R190, E191, E212, R245, H246 and R249 disrupted CP–CP interaction, whereas substitutions at the amino acid positions R188, D189, D198, K205, K218 and D250 did not. These results indicate that the C-terminal region of SMV CP may contain a domain(s) or amino acids required for CP–CP interaction and virus assembly.

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2006-01-01
2019-11-17
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Supplements

vol. , part 1, pp. 225 – 229

Amino acid sequence alignments of the SMV-G7H sequence with other SMV strains and potyviruses.

Interactions among truncated mutants as measured by a yeast two-hybrid assay.

Nucleotide sequences of primers used for alanine-scanning mutagenesis.

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