1887

Abstract

Sequencing of the monopartite RNA genome of a Fijian isolate of Taro vein chlorosis virus (TaVCV) confirmed that it is a definitive rhabdovirus with most similarity to members of the genus . The TaVCV 12 020 nt negative-sense RNA genome contained six ORFs in the antigenomic sequence, equivalent to the N, P, 3, M, G and L genes that have been identified in other rhabdoviruses. The putative gene products had highest similarity to those of the nucleorhabdovirus . A characteristic 3′-AAUUCUUUUUGGGUUGU/A-5′ sequence was identified in each of the intergenic regions and the TaVCV leader and trailer sequences comprised 140 and 61 nt, respectively. Assignment of TaVCV to the genus was supported by thin-section electron microscopy of TaVCV-infected taro leaves, which identified virions budding from nuclear membranes into the perinuclear space. Variability studies identified high levels of TaVCV sequence diversity. Within the L gene of 20 TaVCV isolates from Fiji, the Federated States of Micronesia, New Caledonia, Papua New Guinea, Solomon Islands and Vanuatu, maximum variability at the nucleotide level was 27·4 %. Within the N gene, maximum variability among 15 isolates at the nucleotide level was 19·3 %. The high level of TaVCV variability observed suggested that the introduction of TaVCV to the Pacific Islands was not a recent occurrence.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/vir.0.80591-0
2005-02-01
2019-11-17
Loading full text...

Full text loading...

/deliver/fulltext/jgv/86/2/vir860491.html?itemId=/content/journal/jgv/10.1099/vir.0.80591-0&mimeType=html&fmt=ahah

References

  1. Brunt, A., Crabtree, K. & Gibbs, A. (editors) ( 1990; ). Viruses of Tropical Plants. Descriptions and Lists from the VIDE Database. Wallingford, UK: CAB International.
  2. Chen, X.-Y., Luo, Z.-L. & Fang, R.-X. ( 1998; ). Structure analysis of the rice yellow stunt rhabdovirus gene 3. Chin Sci Bull 43, 745–748 (in Chinese).
    [Google Scholar]
  3. Choi, T.-J., Kuwata, S., Koonin, E. V., Heaton, L. A. & Jackson, A. O. ( 1992; ). Structure of the L (polymerase) protein gene of sonchus yellow net virus. Virology 189, 31–39.[CrossRef]
    [Google Scholar]
  4. Denham, T. P., Haberle, S. G., Lentfer, C., Fullagar, R., Field, J., Therin, M., Porch, N. & Winsborough, B. ( 2003; ). Origins of agriculture at Kuk swamp in the highlands of New Guinea. Science 301, 189–193.[CrossRef]
    [Google Scholar]
  5. Devitt, L. C., Hafner, G., Dale, J. L. & Harding, R. M. ( 2001; ). Partial characterisation of a new dsRNA virus infecting taro. In Proceedings of the First Australian Virology Congress, 5–9 December, 2001, Fraser Island, Australia.
  6. Fang, R.-X., Wang, Q., Xu, B.-Y., Pang, Z., Zhu, H.-T., Mang, K.-Q., Gao, D.-M., Qin, W.-S. & Chua, N.-H. ( 1994; ). Structure of the nucleocapsid protein gene of rice yellow stunt rhabdovirus. Virology 204, 367–375.[CrossRef]
    [Google Scholar]
  7. Gill, D. S. & Banerjee, A. K. ( 1986; ). Complete nucleotide sequence of the matrix protein mRNA of vesicular stomatitis virus (New Jersey serotype). Virology 150, 308–312.[CrossRef]
    [Google Scholar]
  8. Goldberg, K.-B., Modrell, B., Hillman, B. I., Heaton, L. A., Choi, T.-J. & Jackson, A. O. ( 1991; ). Structure of the glycoprotein gene of sonchus yellow net virus, a plant rhabdovirus. Virology 185, 32–38.[CrossRef]
    [Google Scholar]
  9. Goodin, M. M., Austin, J., Tobias, R., Fujita, M., Morales, C. & Jackson, A. O. ( 2001; ). Interactions and nuclear import of the N and P proteins of sonchus yellow net virus, a plant nucleorhabdovirus. J Virol 75, 9393–9406.[CrossRef]
    [Google Scholar]
  10. Goodin, M. M., Dietzgen, R. G., Schichnes, D., Ruzin, S. & Jackson, A. O. ( 2002; ). pGD vectors: versatile tools for the expression of green and red fluorescent protein fusions in agroinfiltrated plant leaves. Plant J 31, 375–383.[CrossRef]
    [Google Scholar]
  11. Hall, J. L. & Hawes, C. (editors) ( 1991; ). Electron Microscopy of Plant Cells. London: Academic Press.
  12. Heaton, L. A., Zuidema, D. & Jackson, A. O. ( 1987; ). Structure of the M2 protein gene of sonchus yellow net virus. Virology 161, 234–241.[CrossRef]
    [Google Scholar]
  13. Heaton, L. A., Hillman, B. I., Hunter, B. G., Zuidema, D. & Jackson, A. O. ( 1989; ). Physical map of the genome of sonchus yellow net virus, a plant rhabdovirus with six genes and conserved gene junction sequences. Proc Natl Acad Sci U S A 86, 8665–8668.[CrossRef]
    [Google Scholar]
  14. Hillman, B. I., Heaton, L. A., Hunter, B. G., Modrell, B. & Jackson, A. O. ( 1990; ). Structure of the gene encoding the M1 protein of sonchus yellow net virus. Virology 179, 201–207.[CrossRef]
    [Google Scholar]
  15. Hogenhout, S. A., Redinbaugh, M. G. & Ammar, E.-D. ( 2003; ). Plant and animal rhabdovirus host range: a bug's view. Trends Microbiol 11, 264–271.[CrossRef]
    [Google Scholar]
  16. Hsu, H. T. & Black, L. M. ( 1968; ). Polyethylene glycol for purification of potato yellow dwarf virus. Phytopathology 63, 692–696.
    [Google Scholar]
  17. Huang, Y., Zhao, H., Luo, Z., Chen, X. & Fang, R.-X. ( 2003; ). Novel structure of the genome of Rice yellow stunt virus: identification of the gene 6-encoded virion protein. J Gen Virol 84, 2259–2264.[CrossRef]
    [Google Scholar]
  18. Jackson, A. O., Francki, R. I. B. & Zuidema, D. ( 1987; ). Biology, structure and replication of plant rhabdoviruses. In The Rhabdoviruses, pp. 427–508. Edited by R. R. Wagner. New York: Plenum.
  19. James, M., Kenten, R. H. & Woods, R. D. ( 1973; ). Virus-like particles associated with two diseases of Colocasia esculenta (L.) Schott in the Solomon Islands. J Gen Virol 21, 145–153.[CrossRef]
    [Google Scholar]
  20. Klerks, M. M., Lindner, J. L., Vaskova, D., Spak, J., Thompson, J. F., Jelkmann, W. & Schoen, C. D. ( 2004; ). Detection and tentative grouping of strawberry crinkle virus isolates. Eur J Plant Pathol 110, 45–52.[CrossRef]
    [Google Scholar]
  21. Koonin, E. V. ( 1991; ). The phylogeny of RNA-dependent RNA polymerases of positive-strand RNA viruses. J Gen Virol 72, 2197–2206.[CrossRef]
    [Google Scholar]
  22. Koonin, E. V. & Dolja, V. V. ( 1993; ). Evolution and taxonomy of positive-strand RNA viruses: implications of comparative analysis of amino acid sequences. Crit Rev Biochem Mol Biol 28, 375–430.[CrossRef]
    [Google Scholar]
  23. Luo, Z. L. & Fang, R. X. ( 1998; ). Structure analysis of the rice yellow stunt rhabdovirus glycoprotein gene and its mRNA. Arch Virol 143, 2453–2459.[CrossRef]
    [Google Scholar]
  24. Luo, Z., Chen, X., Gao, D. & Fang, R. ( 1998; ). The gene 4 of rice yellow stunt rhabdovirus encodes the matrix protein. Virus Genes 16, 277–280.[CrossRef]
    [Google Scholar]
  25. Maino, M. K. ( 2003; ). The development of a serological-based diagnostic test for Dasheen mosaic potyvirus (DsMV). MSc Thesis, School of Life Sciences, Queensland University of Technology, Australia.
  26. Melcher, U. ( 2000; ). The ‘30K’ superfamily of viral movement proteins. J Gen Virol 81, 257–266.
    [Google Scholar]
  27. Neumann, G., Whitt, M. A. & Kawaoka, Y. ( 2002; ). A decade after the generation of a negative-sense RNA virus from cloned cDNA – what have we learned? J Gen Virol 83, 2635–2662.
    [Google Scholar]
  28. Page, R. D. M. ( 1996; ). treeview: an application to display phylogenetic trees on personal computers. Comput Appl Biosci 12, 357–358.
    [Google Scholar]
  29. Pearson, M. N., Jackson, G. V. H., Saelea, J. & Morar, S. G. ( 1999; ). Evidence for two rhabdoviruses in taro (Colocasia esculenta) in the Pacific region. Aust Plant Pathol 28, 248–253.[CrossRef]
    [Google Scholar]
  30. Posthuma, K. I., Adams, A. N., Hong, Y. & Kirby, M. J. ( 2002; ). Detection of strawberry crinkle virus in plant and aphids by RT-PCR using conserved L gene sequences. Plant Pathol 51, 266–274.[CrossRef]
    [Google Scholar]
  31. Rayssiguier, C., Cioe, L., Withers, E., Wunner, W. H. & Curtis, P. J. ( 1986; ). Cloning of rabies virus matrix protein mRNA and determination of its amino acid sequence. Virus Res 5, 177–190.[CrossRef]
    [Google Scholar]
  32. Sasaya, T., Kusaba, S., Ishikawa, K. & Koganezawa, H. ( 2004; ). Nucleotide sequence of RNA2 of Lettuce big-vein virus and evidence for a possible transcription termination/initiation strategy similar to that of rhabdoviruses. J Gen Virol 85, 2709–2717.[CrossRef]
    [Google Scholar]
  33. Scholthof, K.-B. G., Hillman, B. I., Modrell, B., Heaton, L. A. & Jackson, A. O. ( 1994; ). Characterization and detection of sc4: a sixth gene encoded by sonchus yellow net virus. Virology 204, 279–288.[CrossRef]
    [Google Scholar]
  34. Shaw, D. E., Plumb, R. T. & Jackson, G. V. H. ( 1979; ). Virus diseases of taro (Colocasia esculenta) and Xanthosoma sp. in Papua New Guinea. Papua New Guinea Agric J 30, 71–97.
    [Google Scholar]
  35. Tanno, F., Nakatsu, A., Toriyama, S. & Kojima, M. ( 2000; ). Complete nucleotide sequence of Northern cereal mosaic virus and its genome organization. Arch Virol 145, 1373–1384.[CrossRef]
    [Google Scholar]
  36. Teninges, D., Bras, F. & Dezélée, S. ( 1993; ). Genome organization of the Sigma rhabdovirus: six genes and a gene overlap. Virology 193, 1018–1023.[CrossRef]
    [Google Scholar]
  37. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G. ( 1997; ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef]
    [Google Scholar]
  38. Walker, P. J., Benmansour, A., Dietzgen, R. & 7 other authors ( 2000; ). Family Rhabdoviridae. In Virus Taxonomy and Nomenclature of Viruses. Seventh Report of the International Committee on Taxonomy of Viruses, pp. 563–583. Edited by M. H. V. van Regenmortel, C. M. Fauquet, D. H. L. Bishop, E. B. Carstens, M. K. Estes, S. M. Lemon, J. Maniloff, M. A. Mayo, D. J. McGeoch, C. R. Pringle & R. B. Wickner. San Diego: Academic Press.
  39. Wang, Q., Chen, X.-Y., Luo, Z.-L. & Fang, R.-X. ( 1999; ). Sequence analysis of leader and trailer regions of rice yellow stunt rhabdovirus and characterization of their in vivo transcripts. Sci China Ser C Life Sci 42, 50–56.[CrossRef]
    [Google Scholar]
  40. Wetzel, T., Dietzgen, R. G., Geering, A. D. W. & Dale, J. L. ( 1994a; ). Analysis of the nucleocapsid gene of lettuce necrotic yellows rhabdovirus. Virology 202, 1054–1057.[CrossRef]
    [Google Scholar]
  41. Wetzel, T., Dietzgen, R. G. & Dale, J. L. ( 1994b; ). Genomic organization of lettuce necrotic yellows rhabdovirus. Virology 200, 401–412.[CrossRef]
    [Google Scholar]
  42. Yang, I. C., Hafner, G. J., Dale, J. L. & Harding, R. M. ( 2003a; ). Genomic characterization of Taro bacilliform virus. Arch Virol 148, 937–949.[CrossRef]
    [Google Scholar]
  43. Yang, I. C., Hafner, G. J., Revill, P. A., Dale, J. L. & Harding, R. M. ( 2003b; ). Sequence diversity of South Pacific isolates of Taro bacilliform virus and the development of a PCR-based diagnostic test. Arch Virol 148, 1957–1968.[CrossRef]
    [Google Scholar]
  44. Zhu, H.-T., Chen, X.-Y., Luo, Z.-L., Fang, R.-X. & Gao, D.-M. ( 1997; ). Nucleotide sequence of the rice yellow stunt rhabdovirus gene 2. Chin J Virol 13, 369–375 (in Chinese).
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/vir.0.80591-0
Loading
/content/journal/jgv/10.1099/vir.0.80591-0
Loading

Data & Media loading...

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error