1887

Abstract

(JUNV), the causative agent of Argentine haemorrhagic fever, is a human pathogen that naturally enters the body through the epithelial cells of the respiratory and digestive tracts. The interaction of JUNV with two types of polarized epithelial cultures, Vero C1008 and A549, was investigated. Radioactive virus-binding assays showed that JUNV infects polarized lines preferentially through the apical surface. High-level expression of viral nucleoprotein was detected in polarized cell lines infected through the apical domain. Virus production from apical media was about 100-fold higher than that found into the basolateral medium. Confocal-immunofluorescence analysis revealed high-level expression of glycoprotein at the apical-membrane surface. Disruption of the microtubule network by colchicine impaired JUNV vectorial release. This is the first study to analyse the interaction between a member of the virus family and polarized epithelial cells, showing preferential entry and release from the apical plasma membrane.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/vir.0.80473-0
2005-05-01
2019-09-23
Loading full text...

Full text loading...

/deliver/fulltext/jgv/86/5/vir861475.html?itemId=/content/journal/jgv/10.1099/vir.0.80473-0&mimeType=html&fmt=ahah

References

  1. Blau, D. M. & Compans, R. W. ( 1995; ). Entry and release of measles virus are polarized in epithelial cells. Virology 210, 91–99.[CrossRef]
    [Google Scholar]
  2. Bose, S., Malur, A. & Banerjee, A. K. ( 2001; ). Polarity of human parainfluenza virus type 3 infection in polarized human lung epithelial A549 cells: role of microfilament and microtubule. J Virol 75, 1984–1989.[CrossRef]
    [Google Scholar]
  3. Candurra, N. A., Lago, M. J., Maskin, L. & Damonte, E. B. ( 1999; ). Involvement of the cytoskeleton in Junin virus multiplication. J Gen Virol 80, 147–156.
    [Google Scholar]
  4. Cao, W., Henry, M. D., Borrow, P. & 7 other authors ( 1998; ). Identification of α-dystroglycan as a receptor for lymphocytic choriomeningitis virus and Lassa fever virus. Science 282, 2079–2081.[CrossRef]
    [Google Scholar]
  5. Chen, S. Y., Matsuoka, Y. & Compans, R. W. ( 1991; ). Assembly and polarized relese of Punta Toro virus and effects of brefeldin A. J Virol 65, 1427–1439.
    [Google Scholar]
  6. Chu, J. J. H. & Ng, M. L. ( 2002; ). Infection of polarized epithelial cells with flavivirus West Nile: polarized entry and egress of virus occur though the apical surface. J Gen Virol 83, 2427–2435.
    [Google Scholar]
  7. Clayson, E. T., Brando, L. V. J. & Compans, R. W. ( 1989; ). Release of simian virus 40 virions from epithelial cells is polarized and occurs without cell lysis. J Virol 63, 2278–2288.
    [Google Scholar]
  8. Clegg, J. C. ( 2002; ). Molecular phylogeny of the arenaviruses. Curr Top Microbiol Immunol 262, 1–24.
    [Google Scholar]
  9. Contigiani, M. S. & Sabattini, M. S. ( 1977; ). Virulencia diferencial de cepas de virus Junín por marcadores Biológicos en ratones y cobayos. Medicina (B Aires) 37, 244–251 (in Spanish).
    [Google Scholar]
  10. Cordo, S. M. & Candurra, N. A. ( 2003; ). Intermediate filament integrity is required for Junin virus replication. Virus Res 97, 47–55.[CrossRef]
    [Google Scholar]
  11. Damonte, E. B., Mersich, S. E. & Candurra, N. A. ( 1994; ). Intracellular processing and transport of Junin virus glycoproteins influences virion infectivity. Virus Res 34, 317–326.[CrossRef]
    [Google Scholar]
  12. Fuller, S., von Bonsdorff, C.-H. & Simons, K. ( 1984; ). Vesicular stomatitis virus infects and matures only through the basolateral surface of the polarized epithelial cell line, MDCK. Cell 38, 65–77.[CrossRef]
    [Google Scholar]
  13. Gonzalez, P. H., Cossio, P. M., Arana, R., Maiztegui, J. I. & Laguens, R. P. ( 1980; ). Lymphatic tissue in Argentine hemorrhagic fever. Pathologic features. Arch Pathol Lab Med 104, 250–254.
    [Google Scholar]
  14. Guadiz, G., Sporn, L. A., Goss, R. A., Lawrence, S. O., Marder, V. J. & Simpson-Haidaris, P. J. ( 1997; ). Polarized secretion of fibrinogen by lung epithelial cells. Am J Respir Cell Mol Biol 17, 60–69.[CrossRef]
    [Google Scholar]
  15. Jacob, R., Heine, M., Alfalah, M. & Naim, H. Y. ( 2003; ). Distinct cytoskeletal tracks direct individual vesicle populations to the apical membrane of epithelial cells. Curr Biol 13, 607–612.[CrossRef]
    [Google Scholar]
  16. Lafont, F., Burkhardt, J. K. & Simons, K. ( 1994; ). Involvement of microtubule motors in basolateral and apical transport in kidney cells. Nature 372, 801–803.[CrossRef]
    [Google Scholar]
  17. Laguens, M., Chambó, J. G. & Laguens, R. P. ( 1983; ). In vivo replication of pathogenic and attenuated strains of Junin virus in different cell populations of lymphatic tissue. Infect Immun 41, 1279–1283.
    [Google Scholar]
  18. Marozin, S., Prank, U. & Sodeik, B. ( 2004; ). Herpes simplex virus type 1 infection of polarized epithelial cells requires microtubules and access to receptors present at cell–cell contact sites. J Gen Virol 85, 775–786.[CrossRef]
    [Google Scholar]
  19. Neely, J. D., Amiry-Moghaddam, M., Ottersen, O. P., Froehner, S. C., Agre, P. & Adams, M. E. ( 2001; ). Syntrophin-dependent expression and localization of Aquaporin-4 water channel protein. Proc Natl Acad Sci U S A 98, 14108–14113.[CrossRef]
    [Google Scholar]
  20. Parodi, A., Greenway, D., Rugiero, H. R. & 7 other authors ( 1958; ). Sobre la etiología del brote epidémico de Junín. Dia Med 30, 2300–2302 (in Spanish).
    [Google Scholar]
  21. Romanowski, V. ( 1993; ). Genetic organization of Junín virus, the etiological agent of Argentine hemorrhagic fever. In The Arenaviridae, pp. 51–83. Edited by M. S. Salvato. New York: Plenum.
  22. Rossen, J. W. A., Bekker, C. P. J., Voorhout, W. F., Strous, G. J. A. M., van der Ende, A. & Rottier, P. J. M. ( 1994; ). Entry and release of transmissible gastroenteritis coronavirus are restricted to apical surfaces of polarized epithelial cells. J Virol 68, 7966–7973.
    [Google Scholar]
  23. Schneider-Schaulies, J. ( 2000; ). Cellular receptors for viruses: links to tropism and pathogenesis. J Gen Virol 81, 1413–1429.
    [Google Scholar]
  24. Scolaro, L. A., Mersich, S. E. & Damonte, E. B. ( 1989; ). Reduced virulence of a Junin virus mutant is associated with restricted multiplication in murine cells. Virus Res 13, 283–294.[CrossRef]
    [Google Scholar]
  25. Spiropoulou, C. F., Kunz, S., Rollin, P. E., Campbell, K. P. & Oldstone, M. B. A. ( 2002; ). New World arenavirus clade C, but not clade A and B viruses, utilizes α-dystroglycan as its major receptor. J Virol 76, 5140–5146.[CrossRef]
    [Google Scholar]
  26. Srinivas, R. V., Balachandran, N., Alonso-Caplen, F. V. & Compans, R. W. ( 1986; ). Expression of herpes simplex virus glycoproteins in polarized epithelial cells. J Virol 58, 689–693.
    [Google Scholar]
  27. Weissenbacher, M. C., Laguens, R. P. & Coto, C. E. ( 1987; ). Argentine hemorrhagic fever. Curr Top Microbiol Immunol 134, 79–116.
    [Google Scholar]
  28. Zegers, M. M. P., Zaal, K. J. M., van IJzendoorn, S. C. D., Klappe, K. & Hoekstra, D. ( 1998; ). Actin filaments and microtubules are involved in different membrane traffic pathways that transport sphingolipids to the apical surface of polarized HepG2 cells. Mol Biol Cell 9, 1939–1949.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/vir.0.80473-0
Loading
/content/journal/jgv/10.1099/vir.0.80473-0
Loading

Data & Media loading...

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error