1887

Abstract

MicroRNAs (miRNAs) are ∼21–25 nt long and interact with mRNAs to lead to either translational repression or RNA cleavage through RNA interference. A previous study showed that human immunodeficiency virus 1 (HIV-1) dsRNA from AIDS patients who are long-term non-progressors inhibited HIV-1 transcription. In the study reported here, -derived miRNAs in HIV-1-infected and transduced cells were identified, and showed that HIV-1 transcription was suppressed by -expressing miRNA, miR-N367, in human T cells. The miR-N367 could reduce HIV-1 LTR promoter activity through the negative responsive element of the U3 region in the 5′-LTR. Therefore, miRNA produced in HIV-1-infected cells may downregulate HIV-1 transcription through both a post-transcriptional pathway and a transcriptional neo-pathway.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/vir.0.80449-0
2005-03-01
2019-11-21
Loading full text...

Full text loading...

/deliver/fulltext/jgv/86/3/vir860751.html?itemId=/content/journal/jgv/10.1099/vir.0.80449-0&mimeType=html&fmt=ahah

References

  1. Aravin, A. A., Naumova, N. M., Tulin, A. V., Vagin, V. V., Rozovsky, Y. M. & Gvozdev, V. A. ( 2001; ). Double-stranded RNA-mediated silencing of genomic tandem repeats and transposable elements in the D. melanogaster germline. Curr Biol 11, 1017–1027.[CrossRef]
    [Google Scholar]
  2. Baulcombe, D. C. ( 2001; ). RNA silencing. Diced defence. Nature 409, 295–296.[CrossRef]
    [Google Scholar]
  3. Boden, D., Pusch, O., Silbermann, R., Lee, F., Tucker, L. & Ramratnam, B. ( 2004; ). Enhanced gene silencing of HIV-1 specific siRNA using microRNA designed hairpins. Nucleic Acids Res 32, 1154–1158.[CrossRef]
    [Google Scholar]
  4. Brisibe, E. A., Okada, N., Mizukami, H., Okuyama, H. & Fujii, Y. R. ( 2003; ). RNA interference: potentials for the prevention of HIV infections and the challenges ahead. Trends Biotechnol 21, 306–311.[CrossRef]
    [Google Scholar]
  5. Cullen, B. R., Lomedico, P. T. & Ju, G. ( 1984; ). Transcriptional interference in avian retrovirus – implication for the promoter insertion model of leukaemogenesis. Nature 307, 241–245.[CrossRef]
    [Google Scholar]
  6. D'Aloja, P., Olivetta, E., Bona, R., Nappi, F., Pedacchia, D., Pugliese, K., Ferrari, G., Verani, P. & Federico, M. ( 1998; ). gag, vif, and nef genes contribute to the homologous viral interference induced by a nonproducer human immunodeficiency virus type 1 (HIV-1) variant: identification of novel HIV-1-inhibiting viral protein mutants. J Virol 72, 4308–4319.
    [Google Scholar]
  7. Das, A. T., Brummelkamp, T. R., Westerhout, E. M., Vink, M., Madiredjo, M., Bernards, R. & Berkhout, B. ( 2004; ). Human immunodeficiency virus type 1 escapes from RNA interference-mediated inhibition. J Virol 78, 2601–2605.[CrossRef]
    [Google Scholar]
  8. Deacon, N. J., Tsykin, A., Solomon, A. & 17 other authors ( 1995; ). Genomic structure of an attenuated quasi species of HIV-1 from a blood transfusion donor and recipients. Science 270, 988–991.[CrossRef]
    [Google Scholar]
  9. Doench, J. G., Petersen, C. P. & Sharp, P. A. ( 2003; ). siRNAs can function as miRNAs. Genes Dev 17, 438–442.[CrossRef]
    [Google Scholar]
  10. Elbashir, S. M., Lendeckel, W. & Tuschl, T. ( 2001; ). RNA interference is mediated by 21- and 22-nucleotide RNAs. Genes Dev 15, 188–200.[CrossRef]
    [Google Scholar]
  11. Fire, A., Xu, S., Montgomery, M. K., Kostas, S. A., Driver, S. E. & Mello, C. C. ( 1998; ). Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans. Nature 391, 806–811.[CrossRef]
    [Google Scholar]
  12. Fish, R. J. & Kruithof, E. K. ( 2004; ). Short-term cytotoxic effects and long-term instability of RNAi delivered using lentiviral vectors. BMC Mol Biol 5, 9.[CrossRef]
    [Google Scholar]
  13. Gottesman, S. ( 2002; ). Stealth regulation: biological circuits with small RNA switches. Genes Dev 16, 2829–2842.[CrossRef]
    [Google Scholar]
  14. Grad, Y., Aach, J., Hayes, G. D., Reinhart, B. J., Church, G. M., Ruvkun, G. & Kim, J. ( 2003; ). Computational and experimental identification of C. elegans microRNAs. Mol Cell 11, 1253–1263.[CrossRef]
    [Google Scholar]
  15. Hatama, S., Otake, K., Omoto, S., Murase, Y., Ikemoto, A., Mochizuki, M., Takahashi, E., Okuyama, H. & Fujii, Y. R. ( 2001; ). Isolation and sequencing of infectious clones of feline foamy virus and human/feline foamy virus Env chimera. J Gen Virol 82, 2999–3004.
    [Google Scholar]
  16. Hill, C. L., Bieniasz, P. D. & McClure, M. O. ( 1999; ). Properties of human foamy virus relevant to its development as a vector for gene therapy. J Gen Virol 80, 2003–2009.
    [Google Scholar]
  17. Hobert, O. ( 2004; ). Common logic of transcription factor and microRNA action. Trends Biochem Sci 29, 462–468.[CrossRef]
    [Google Scholar]
  18. Jacque, J. M., Triques, K. & Stevenson, M. ( 2002; ). Modulation of HIV-1 replication by RNA interference. Nature 418, 435–438.[CrossRef]
    [Google Scholar]
  19. Kawai, M., He, L., Kawamura, T., Omoto, S., Fujii, Y. R. & Okada, N. ( 2003; ). Chimeric human/murine monoclonal IgM antibodies to HIV-1 Nef antigen expressed on chronically infected cells. Microbiol Immunol 47, 247–253.[CrossRef]
    [Google Scholar]
  20. Kestler, H. W., III, Ringler, D. J., Mori, K., Panicali, D. L., Sehgal, P. K., Daniel, M. D. & Desrosiers, R. C. ( 1991; ). Importance of the nef gene for maintenance of high virus loads and for development of AIDS. Cell 65, 651–662.[CrossRef]
    [Google Scholar]
  21. Ketting, R. F., Haverkamp, T. H., van Luenen, H. G. & Plasterk, R. H. ( 1999; ). Mut-7 of C. elegans, required for transposon silencing and RNA interference, is a homolog of Werner syndrome helicase and RNaseD. Cell 99, 133–141.[CrossRef]
    [Google Scholar]
  22. Kirchhoff, F., Greenough, T. G., Brettler, D. B., Sullivan, J. L. & Desrosiers, R. C. ( 1995; ). Brief report: absence of intact nef sequences in a long-term survivor with nonprogressive HIV-1 infection. N Engl J Med 332, 228–232.[CrossRef]
    [Google Scholar]
  23. Learmont, J. C., Geczy, A. F., Mills, J. & 9 other authors ( 1999; ). Immunologic and virologic status after 14 to 18 years of infection with an attenuated strain of HIV-1. A report from the Sydney Blood Bank Cohort. N Engl J Med 340, 1715–1722.[CrossRef]
    [Google Scholar]
  24. Lee, Y., Jeon, K., Lee, J. T., Kim, S. & Kim, V. N. ( 2002; ). MicroRNA maturation: stepwise processing and subcellular localization. EMBO J 21, 4663–4670.[CrossRef]
    [Google Scholar]
  25. Lee, Y., Ahn, C., Han, J. & 8 other authors ( 2003; ). The nuclear RNase III Drosha initiates microRNA processing. Nature 425, 415–419.[CrossRef]
    [Google Scholar]
  26. Miyagishi, M., Sumimoto, H., Miyoshi, H., Kawakami, Y. & Taira, K. ( 2004; ). Optimization of an siRNA-expression system with an improved hairpin and its significant suppressive effects in mammalian cells. J Gene Med 6, 715–723.[CrossRef]
    [Google Scholar]
  27. Nishitsuji, H., Ikeda, T., Miyoshi, H., Ohashi, T., Kannagi, M. & Masuda, T. ( 2004; ). Expression of small hairpin RNA by lentivirus-based vector confers efficient and stable gene-suppression of HIV-1 on human cells including primary non-dividing cells. Microbes Infect 6, 76–85.[CrossRef]
    [Google Scholar]
  28. Olivetta, E., Pugliese, K., Bona, R., D'Aloja, P., Ferrantelli, F., Santarcangelo, A. C., Mattia, G., Verani, P. & Federico, M. ( 2000; ). cis expression of the F12 human immunodeficiency virus (HIV) Nef allele transforms the highly productive NL4-3 HIV type 1 to a replication-defective strain: involvement of both Env gp41 and CD4 intracytoplasmic tails. J Virol 74, 483–492.[CrossRef]
    [Google Scholar]
  29. Omoto, S., Brisibe, E. A., Okuyama, H. & Fujii, Y. R. ( 2004a; ). Feline foamy virus Tas protein is a DNA-binding transactivator. J Gen Virol 85, 2931–2935.[CrossRef]
    [Google Scholar]
  30. Omoto, S., Ito, M., Tsutsumi, Y., Ichikawa, Y., Okuyama, H., Brisibe, E. A., Saksena, N. K. & Fujii, Y. R. ( 2004b; ). HIV-1 nef suppression by virally encoded microRNA. Retrovirology 1, 44.[CrossRef]
    [Google Scholar]
  31. Pfeffer, S., Zavolan, M., Grasser, F. A. & 8 other authors ( 2004; ). Identification of virus-encoded microRNAs. Science 304, 734–736.[CrossRef]
    [Google Scholar]
  32. Robert-Guroff, M., Popovic, M., Gartner, S., Markham, P., Gallo, R. C. & Reitz, M. S. ( 1990; ). Structure and expression of tat-, rev-, and nef-specific transcripts of human immunodeficiency virus type 1 in infected lymphocytes and macrophages. J Virol 64, 3391–3398.
    [Google Scholar]
  33. Sijen, T. & Plasterk, R. H. ( 2003; ). Transposon silencing in the Caenorhabditis elegans germ line by natural RNAi. Nature 426, 310–314.[CrossRef]
    [Google Scholar]
  34. Sunkar, R. & Zhu, J. K. ( 2004; ). Novel and stress-regulated microRNAs and other small RNAs from Arabidopsis. Plant Cell 16, 2001–2019.[CrossRef]
    [Google Scholar]
  35. Tabara, H., Sarkissian, M., Kelly, W. G., Fleenor, J., Grishok, A., Timmons, L., Fire, A. & Mello, C. C. ( 1999; ). The rde-1 gene, RNA interference, and transposon silencing in C. elegans. Cell 99, 123–132.[CrossRef]
    [Google Scholar]
  36. Vella, M. C., Choi, E. Y., Lin, S. Y., Reinert, K. & Slack, F. J. ( 2004; ). The C. elegans microRNA let-7 binds to imperfect let-7 complementary sites from the lin-41 3′UTR. Genes Dev 18, 132–137.[CrossRef]
    [Google Scholar]
  37. Yamamoto, T., Omoto, S., Mizuguchi, M. & 7 other authors ( 2002; ). Double-stranded nef RNA interferes with human immunodeficiency virus type 1 replication. Microbiol Immunol 46, 809–817.[CrossRef]
    [Google Scholar]
  38. Yekta, S., Shih, I. & Bartel, D. P. ( 2004; ). MicroRNA-directed cleavage of HOXB8 mRNA. Science 304, 594–596.[CrossRef]
    [Google Scholar]
  39. Zeng, Y., Yi, R. & Cullen, B. R. ( 2003; ). MicroRNAs and small interfering RNAs can inhibit mRNA expression by similar mechanisms. Proc Natl Acad Sci U S A 100, 9779–9784.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/vir.0.80449-0
Loading
/content/journal/jgv/10.1099/vir.0.80449-0
Loading

Data & Media loading...

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error