1887

Abstract

Cherry chlorotic rusty spot (CCRS) and Amasya cherry disease (ACD) display similar symptoms and are associated with a series of dsRNAs. However, a direct comparison has been lacking. Here, a side-by-side analysis confirmed that both diseases were symptomatologically very similar, as were the number (10–12) and size of their associated dsRNAs. Sequence determination of four of these dsRNAs revealed that they were essentially identical for CCRS and ACD. The largest (3399 bp), which potentially encoded a protein of 1087 aa with the eight motifs conserved in RNA-dependent RNA polymerases of dsRNA mycoviruses, had the highest similarity to those coded by dsRNA 1 of viruses belonging to the genus and was termed CCRS or ACD chrys-dsRNA 1. The three closely migrating dsRNAs had the properties of the other components of a chrysovirus and in CCRS and ACD versions, respectively, were chrys-dsRNA 2 (3125 and 3128 bp), chrys-dsRNA 3 (2833 bp) and chrys-dsRNA 4 (2499 and 2498 bp), potentially encoding the major capsid protein (993 and 994 aa) and two proteins (884 and 677 aa, respectively) of unknown function. The four 5′- and 3′-UTRs shared internal similarities and had conserved GAAAAUUAUGG and AUAUGC termini, respectively. The 5′-UTRs contained the ‘Box 1’ motif followed by a stretch rich in CAA, CAAA and CAAAA repeats, characteristic of chrysovirus dsRNAs. Because species of the genus have only been described as infecting fungi, this suggests a fungal aetiology for CCRS and ACD, a proposal supported by the properties of two other CCRS- and ACD-associated dsRNAs (see accompanying paper by Coutts ., 2004 , in this issue).

Loading

Article metrics loading...

/content/journal/jgv/10.1099/vir.0.80181-0
2004-11-01
2019-10-13
Loading full text...

Full text loading...

/deliver/fulltext/jgv/85/11/vir853389.html?itemId=/content/journal/jgv/10.1099/vir.0.80181-0&mimeType=html&fmt=ahah

References

  1. Açıkgöz, S., Doken, M. T., Citir, A. & Bostan, H. ( 1994; ). The analysis of double stranded (ds) RNA for the diagnosis of the causal agent of Amasya cherry disease in Turkey. EMBO–INRA Workshop Plant and Viruses: Partners in Pathogenicity, Grignon, abstract 36.
  2. Alioto, D., Zaccaria, F., Covelli, L., Ragozzino, A., Di Serio, F. & Milne, R. G. ( 2003; ). Light and electron microscope observations on chlorotic rusty spot, a disorder of cherry in Italy. J Plant Pathol 85, 215–218.
    [Google Scholar]
  3. Blodgett, E. C., Ismen, F. H., Gomec, B., Kalay, K., Altinyay, N. & Wagnon, H. K. ( 1970; ). La maladie d'Amasya du cerisier en Turquie. FAO Plant Prot Bull 18, 49–52.
    [Google Scholar]
  4. Bruenn, J. A. ( 1993; ). A closely related group of RNA-dependent RNA polymerases from double-stranded RNA viruses. Nucleic Acids Res 21, 5667–5669.[CrossRef]
    [Google Scholar]
  5. Castón, J. R., Ghabrial, S. A., Jiang, D., Rivas, G., Alfonso, C., Roca, R., Luque, D. & Carrascosa, J. L. ( 2003; ). Three-dimensional structure of Penicillium chrysogenum virus: a double-stranded RNA virus with a genuine T=1 capsid. J Mol Biol 331, 417–431.[CrossRef]
    [Google Scholar]
  6. Citir, A. ( 1987; ). Preliminary investigations about identity of the causal agent of Amasya cherry disease in Turkey. J Turk Phytopathol 16, 23–34.
    [Google Scholar]
  7. Coutts, R. H. A. & Livieratos, I. C. ( 2003; ). A rapid method for sequencing the 5′- and 3′-termini of dsRNA viral templates using RLM-RACE. J Phytopathol 151, 525–527.[CrossRef]
    [Google Scholar]
  8. Coutts, R. H. A., Covelli, L., Di Serio, F., Citir, A., Açıkgöz, S., Hernández, C., Ragozzino, A. & Flores, R. ( 2004; ). Cherry chlorotic rusty spot and Amasya cherry diseases are associated with a complex pattern of mycoviral-like double-stranded RNAs. II. Characterization of a new species in the genus Partitivirus. J Gen Virol 85, 000–000.
    [Google Scholar]
  9. Di Serio, F., Flores, R. & Ragozzino, A. ( 1996; ). Cherry chlorotic rusty spot: description of a virus-like disease from cherry and studies on its etiological agent. Plant Dis 80, 1203–1206.[CrossRef]
    [Google Scholar]
  10. Di Serio, F., Daròs, J. A., Ragozzino, A. & Flores, R. ( 1997; ). A 451-nt circular RNA from cherry with hammerhead ribozymes in its strands of both polarities. J Virol 71, 6603–6610.
    [Google Scholar]
  11. Di Serio, F., Alioto, D., Ragozzino, A. & Flores, R. ( 1998; ). Both the small circular RNAs and the double-stranded RNAs associated with the chlorotic rusty spot disease of sweet cherry are also found in sour cherry with similar symptoms. Acta Hortic 472, 291–297.
    [Google Scholar]
  12. Gallie, D. R. ( 2002; ). The 5′-leader of tobacco mosaic virus promotes translation through enhanced recruitment of eIF4F. Nucleic Acids Res 30, 3401–3411.[CrossRef]
    [Google Scholar]
  13. Gallie, D. R. & Walbot, V. ( 1992; ). Identification of the motifs within the tobacco mosaic virus 5′-leader responsible for enhancing translation. Nucleic Acids Res 20, 4631–4638.[CrossRef]
    [Google Scholar]
  14. Ghabrial, S. A. ( 1994; ). New developments in fungal virology. Adv Virus Res 43, 303–388.
    [Google Scholar]
  15. Ghabrial, S. A. ( 1998; ). Origin, adaptation and evolutionary pathways of fungal viruses. Virus Genes 16, 119–131.[CrossRef]
    [Google Scholar]
  16. Ghabrial, S. A. & Castón, J. R. ( 2004; ). Chrysoviridae. In Virus Taxonomy. Eighth Report of the International Committee on Taxonomy of Viruses. Edited by C. M. Fauquet, M. A. Mayo, J. Maniloff, U. Desselberger & A. L. Ball. London: Elsevier/Academic Press (in press).
  17. Ghabrial, S. A., Soldevila, A. I. & Havens, W. M. ( 2002; ). Molecular genetics of the viruses infecting the plant pathogenic fungus Helminthosporium victoriae. In Molecular Biology of RNA: Concepts and Applications in Agriculture, Forestry and Medicine, pp. 213–236. Edited by S. Tavantzis. Boca Raton, FL: CRC Press.
  18. Ghabrial, S. A., Buck, K. W., Hillman, B. I. & Milne, R. G. ( 2004; ). Partitiviridae. In Virus Taxonomy. Eighth Report of the International Committee on Taxonomy of Viruses. Edited by C. M. Fauquet, M. A. Mayo, J. Maniloff, U. Desselberger & A. L. Ball. London: Elsevier/Academic Press (in press).
  19. Isogai, M., Uyeda, I. & Hataya, T. ( 1998; ). An efficient cloning strategy for viral double-stranded RNAs with unknown sequences. Ann Phytopathol Soc Jpn 64, 244–248.[CrossRef]
    [Google Scholar]
  20. Jiang, D. & Ghabrial, S. A. ( 2004; ). Molecular characterization of Penicillium chrysogenum virus: reconsideration of the taxonomy of the genus Chrysovirus. J Gen Virol 85, 2111–2121.[CrossRef]
    [Google Scholar]
  21. Kadaré, G. & Haenni, A. L. ( 1997; ). Virus-encoded RNA helicases. J Virol 71, 2583–2590.
    [Google Scholar]
  22. Kozak, M. ( 1986; ). Point mutations define a sequence flanking the AUG initiator codon that modulates translation by eukaryotic ribosomes. Cell 44, 283–292.[CrossRef]
    [Google Scholar]
  23. Kumar, S., Tamura, K., Jakobsen, I. B. & Nei, M. ( 2001; ). mega 2: molecular evolutionary genetics analysis software. Bioinformatics 17, 1244–1245.[CrossRef]
    [Google Scholar]
  24. Lütcke, H. A., Chow, K. C., Mickel, F. S., Moss, K. A., Kern, H. F. & Scheele, G. A. ( 1987; ). Selection of AUG initiation codons differs in plants and animals. EMBO J 6, 43–48.
    [Google Scholar]
  25. Makarova, K. S., Aravind, L. & Koonin, E. V. ( 2000; ). A novel superfamily of predicted cysteine proteases from eukaryotes, viruses and Chlamydia pneumoniae. Trends Biochem Sci 25, 50–52.[CrossRef]
    [Google Scholar]
  26. McCabe, P. M., Pfeiffer, P. & Van Alfen, N. K. ( 1999; ). The influence of dsRNA viruses on the biology of plant pathogenic fungi. Trends Microbiol 7, 377–381.[CrossRef]
    [Google Scholar]
  27. Morris, T. J. & Dodds, J. A. ( 1979; ). Isolation and analysis of double-stranded RNA from virus-infected plant and fungal tissue. Phytopathology 69, 854–858.[CrossRef]
    [Google Scholar]
  28. Navarro, B., Daròs, J. A. & Flores, R. ( 1998; ). Reverse transcription polymerase chain reaction protocols for cloning small circular RNAs. J Virol Methods 73, 1–9.[CrossRef]
    [Google Scholar]
  29. Nuss, D. L. ( 1996; ). Using hypoviruses to probe and perturb signal transduction process underlying fungal pathogenesis. Plant Cell 8, 1845–1853.[CrossRef]
    [Google Scholar]
  30. Pallás, V., Navarro, A. & Flores, R. ( 1987; ). Isolation of a viroid-like RNA from hop different from hop stunt viroid. J Gen Virol 68, 3201–3205.[CrossRef]
    [Google Scholar]
  31. Sambrook, J., Fritsch, E. F. & Maniatis, T. ( 1989; ). Molecular Cloning: a Laboratory Manual, 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory.
  32. Sanderlin, R. S. & Ghabrial, S. A. ( 1978; ). Physiochemical properties of two distinct types of virus-like particles from Helminthosporium victoriae. Virology 87, 142–151.[CrossRef]
    [Google Scholar]
  33. Thompson, J. D., Gibson, T. J., Plewniak, F. & Higgins, D. G. ( 1997; ). The clustal x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef]
    [Google Scholar]
  34. Vreede, F. T., Cloete, M., Napier, G. B., Van Dijk, A. A. & Viljoen, G. J. ( 1998; ). Sequence-independent amplification and cloning of large dsRNA virus genome segments by poly (dA)-oligonucleotide ligation. J Virol Methods 72, 243–247.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/vir.0.80181-0
Loading
/content/journal/jgv/10.1099/vir.0.80181-0
Loading

Data & Media loading...

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error