1887

Abstract

The iral hree-prime epair onuclease (-) gene of the multicapsid nucleopolyhedrovirus (AgMNPV) is the first baculovirus gene to be described with significant homology to a 3′ exonuclease. - is an early gene that is expressed by AgMNPV from 3 h post-infection. In the present study, the AgMNPV - ORF was cloned into the baculovirus multicapsid nucleopolyhedrovirus (AcMNPV) under the control of a polyhedrin promoter. The resulting virus produced an abundant, soluble protein that migrated with an apparent size of 23·7 kDa. The 3′ to 5′ exonuclease activity associated with this --expressing recombinant AcMNPV was 2000-fold above that of wild-type AcMNPV. This exonuclease activity was inhibited by EDTA and was activated in the presence of Mg and, to a lesser extent, Mn. From these results, the AgMNPV - gene is concluded to encode an independently active 3′ to 5′ exonuclease.

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2004-10-01
2020-01-18
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