1887

Abstract

Comparisons of the relative activities of 11 intergenic region (IGR) internal ribosome entry site (IRES) elements of insect with 5′ IRES elements of the hepatitis C and encephalomyocarditis viruses were performed in insect and mammalian cells. Dual luciferase assays were performed to determine the most effective dicistrovirus IGR IRES in the lepidopteran cell lines Sf9 () and BmN (), and the dipteran cell lines S2 () and ATC-10 (). Evaluation of dual luciferase expression from DNA plasmids and -transcribed RNA revealed apparent splicing with certain IRES elements. Though IRES activity depended upon the cell line examined, the black queen cell and C dicistrovirus intergenic IRES elements were most effective for coupled gene expression in the diverse insect cell lines examined.

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2008-12-01
2019-11-17
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