1887

Abstract

In 2003, we described the first human G6P[6] rotavirus strain (B1711). To investigate further the molecular origin of this strain and to determine the possible reassortments leading to this new gene constellation, the complete genome of strain B1711 was sequenced. SimPlot analyses were conducted to compare strain B1711 with other known rotavirus gene segments, and phylogenetic dendrograms were constructed to analyse the origin of the eleven genome segments of strain B1711. Our analysis indicated that strain B1711 acquired its VP1-, VP2-, VP4-, VP6- and NSP1–5-encoding gene segments from human DS-1-like P[6] rotavirus strains, and its VP3 and VP7 gene segments from a bovine rotavirus strain through reassortment. The introduction of animal–human reassortant strains, which might arise in either of the hosts, into the human rotavirus population is an important mechanism for the generation of rotavirus diversity, and might be a challenge for the current rotavirus vaccines and vaccines under development.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/vir.0.2008/003780-0
2008-10-01
2019-12-11
Loading full text...

Full text loading...

/deliver/fulltext/jgv/89/10/2630.html?itemId=/content/journal/jgv/10.1099/vir.0.2008/003780-0&mimeType=html&fmt=ahah

References

  1. Altschul, S. F., Gish, W., Miller, W., Myers, E. W. & Lipman, D. J. ( 1990; ). Basic local alignment search tool. J Mol Biol 215, 403–410.[CrossRef]
    [Google Scholar]
  2. Bányai, K., Gentsch, J. R., Griffin, D. D., Holmes, J. L., Glass, R. I. & Szucs, G. ( 2003; ). Genetic variability among serotype G6 human rotaviruses: identification of a novel lineage isolated in Hungary. J Med Virol 71, 124–134.[CrossRef]
    [Google Scholar]
  3. Estes, M. & Kapikian, A. ( 2007; ). Rotaviruses. In Fields Virology, 5th edn, vol. 2, pp. 1917–1974. Edited by D. M. Knipe, P. M. Howley, D. E. Griffin, R. A. Lamb, M. A. Martin, B. Roizman & S. E. Straus. Philadelphia, PA: Kluwer/Lippincott, Williams and Wilkins.
  4. Gerna, G., Sarasini, A., Parea, M., Arista, S., Miranda, P., Brussow, H., Hoshino, Y. & Flores, J. ( 1992; ). Isolation and characterization of two distinct human rotavirus strains with G6 specificity. J Clin Microbiol 30, 9–16.
    [Google Scholar]
  5. Griffin, D. D., Nakagomi, T., Hoshino, Y., Nakagomi, O., Kirkwood, C. D., Parashar, U. D., Glass, R. I. & Gentsch, J. R. ( 2002; ). Characterization of nontypeable rotavirus strains from the United States: identification of a new rotavirus reassortant (P2A[6],G12) and rare P3[9] strains related to bovine rotaviruses. Virology 294, 256–269.[CrossRef]
    [Google Scholar]
  6. Heaton, P. M. & Ciarlet, M. ( 2007; ). Vaccines: the pentavalent rotavirus vaccine: discovery to licensure and beyond. Clin Infect Dis 45, 1618–1624.[CrossRef]
    [Google Scholar]
  7. Hoshino, Y., Saif, L. J., Kang, S. Y., Sereno, M. M., Chen, W. K. & Kapikian, A. Z. ( 1995; ). Identification of group A rotavirus genes associated with virulence of a porcine rotavirus and host range restriction of a human rotavirus in the gnotobiotic piglet model. Virology 209, 274–280.[CrossRef]
    [Google Scholar]
  8. Ito, H., Sugiyama, M., Masubuchi, K., Mori, Y. & Minamoto, N. ( 2001; ). Complete nucleotide sequence of a group A avian rotavirus genome and a comparison with its counterparts of mammalian rotaviruses. Virus Res 75, 123–138.[CrossRef]
    [Google Scholar]
  9. Khamrin, P., Maneekarn, N., Peerakome, S., Chan-it, W., Yagyu, F., Okitsu, S. & Ushijima, H. ( 2007; ). Novel porcine rotavirus of genotype P[27] shares new phylogenetic lineage with G2 porcine rotavirus strain. Virology 361, 243–252.[CrossRef]
    [Google Scholar]
  10. Kumar, S., Tamura, K., Jakobsen, I. B. & Nei, M. ( 2001; ). mega2: molecular evolutionary genetics analysis software. Bioinformatics 17, 1244–1245.[CrossRef]
    [Google Scholar]
  11. Lambden, P. R., Cooke, S. J., Caul, E. O. & Clarke, I. N. ( 1992; ). Cloning of noncultivatable human rotavirus by single primer amplification. J Virol 66, 1817–1822.
    [Google Scholar]
  12. Martella, V., Ciarlet, M., Bányai, K., Lorusso, E., Cavalli, A., Corrente, M., Elia, G., Arista, S., Camero, M. & other authors ( 2006; ). Identification of a novel VP4 genotype carried by a serotype G5 porcine rotavirus strain. Virology 346, 301–311.[CrossRef]
    [Google Scholar]
  13. Martella, V., Ciarlet, M., Bányai, K., Lorusso, E., Arista, S., Lavazza, A., Pezzotti, G., Decaro, N., Cavalli, A. & other authors ( 2007; ). Identification of group A porcine rotavirus strains bearing a novel VP4 (P) genotype in Italian swine herds. J Clin Microbiol 45, 577–580.[CrossRef]
    [Google Scholar]
  14. Matthijnssens, J., Rahman, M., Martella, V., Xuelei, Y., De Vos, S., De Leener, K., Ciarlet, M., Buonavoglia, C. & Van Ranst, M. ( 2006a; ). Full genomic analysis of human rotavirus strain B4106 and lapine rotavirus strain 30/96 provides evidence for interspecies transmission. J Virol 80, 3801–3810.[CrossRef]
    [Google Scholar]
  15. Matthijnssens, J., Rahman, M., Yang, X., Delbeke, T., Arijs, I., Kabue, J. P., Muyembe, J. J. & Van Ranst, M. ( 2006b; ). G8 rotavirus strains isolated in the Democratic Republic of Congo belong to the DS-1-like genogroup. J Clin Microbiol 44, 1801–1809.[CrossRef]
    [Google Scholar]
  16. Matthijnssens, J., Ciarlet, M., Heiman, E., Arijs, I., Delbeke, T., McDonald, S. M., Palombo, A. E., Iturriza-Gómara, M., Maes, P. & other authors ( 2008a; ). Full genome-based classification of rotaviruses reveals common origin between human Wa-like and porcine rotavirus strains and human DS-1-like and bovine rotavirus strains. J Virol 82, 3204–3219.[CrossRef]
    [Google Scholar]
  17. Matthijnssens, J., Rahman, M., Ciarlet, M. & Van Ranst, M. ( 2008b; ). Emerging human rotavirus genotypes. In Viruses in the Environment, pp. 171–219. Edited by A. E. Palombo & C. D. Kirkwood. Trivandrum, India: Research Signpost.
  18. Nicholas, K. B., Nicholas, H. B., Jr & Deerfield, D. W., II ( 1997; ). GeneDoc: analysis and visualization of genetic variation. EMBnet News 4 (2), 1–4. http://www.embnet.org/files/shared/EMBnetNews/embnet_news_4_2.pdf
  19. Palombo, E. A. & Bishop, R. F. ( 1995; ). Genetic and antigenic characterization of a serotype G6 human rotavirus isolated in Melbourne, Australia. J Med Virol 47, 348–354.[CrossRef]
    [Google Scholar]
  20. Rahman, M., De Leener, K., Goegebuer, T., Wollants, E., Van der Donck, I., Van Hoovels, L. & Van Ranst, M. ( 2003; ). Genetic characterization of a novel, naturally occurring recombinant human G6P[6] rotavirus. J Clin Microbiol 41, 2088–2095.[CrossRef]
    [Google Scholar]
  21. Rahman, M., Matthijnssens, J., Nahar, S., Podder, G., Sack, D. A., Azim, T. & Van Ranst, M. ( 2005; ). Characterization of a novel P[25],G11 human group a rotavirus. J Clin Microbiol 43, 3208–3212.[CrossRef]
    [Google Scholar]
  22. Rahman, M., Matthijnssens, J., Yang, X., Delbeke, T., Arijs, I., Taniguchi, K., Iturriza-Gómara, M., Iftekharuddin, N., Azim, T. & Van Ranst, M. ( 2007; ). Evolutionary history and global spread of the emerging G12 human rotaviruses. J Virol 81, 2382–2390.[CrossRef]
    [Google Scholar]
  23. Rao, C. D., Gowda, K. & Reddy, B. S. ( 2000; ). Sequence analysis of VP4 and VP7 genes of nontypeable strains identifies a new pair of outer capsid proteins representing novel P and G genotypes in bovine rotaviruses. Virology 276, 104–113.[CrossRef]
    [Google Scholar]
  24. Santos, N. & Hoshino, Y. ( 2005; ). Global distribution of rotavirus serotypes/genotypes and its implication for the development and implementation of an effective rotavirus vaccine. Rev Med Virol 15, 29–56.[CrossRef]
    [Google Scholar]
  25. Small, C., Barro, M., Brown, T. L. & Patton, J. T. ( 2007; ). Genome heterogeneity of SA11 rotavirus due to reassortment with “O” agent. Virology 359, 415–424.[CrossRef]
    [Google Scholar]
  26. Steyer, A., Poljsak-Prijatelj, M., Barlic-Maganja, D., Jamnikar, U., Mijovski, J. Z. & Marin, J. ( 2007; ). Molecular characterization of a new porcine rotavirus P genotype found in an asymptomatic pig in Slovenia. Virology 359, 275–282.[CrossRef]
    [Google Scholar]
  27. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G. ( 1997; ). The clustal_x Windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef]
    [Google Scholar]
  28. Van Damme, P., Giaquinto, C., Maxwell, M., Todd, P. & Van der Wielen, M. ( 2007; ). Distribution of rotavirus genotypes in Europe, 2004–2005: the REVEAL Study. J Infect Dis 195 (Suppl 1), S17–S25.[CrossRef]
    [Google Scholar]
  29. Vesikari, T., Karvonen, A., Prymula, R., Schuster, V., Tejedor, J. C., Cohen, R., Meurice, F., Han, H. H., Damaso, S. & Bouckenooghe, A. ( 2007; ). Efficacy of human rotavirus vaccine against rotavirus gastroenteritis during the first 2 years of life in European infants: randomised, double-blind controlled study. Lancet 370, 1757–1763.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/vir.0.2008/003780-0
Loading
/content/journal/jgv/10.1099/vir.0.2008/003780-0
Loading

Data & Media loading...

Supplements

Primers used to amplify the VP1–4, VP6, VP7 and NSP1–5 gene segments described in this study [PDF](54 KB)

PDF

[single PDF](211 KB)

PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error