1887

Abstract

Vector insect cells infected with Rice dwarf virus had vesicular compartments containing viral particles located adjacent to the viroplasm when examined by transmission electron and confocal microscopy. Such compartments were often at the periphery of infected cells. Inhibitors of vesicular transport, brefeldin A and monensin, and an inhibitor of myosin motor activity, butanedione monoxime, abolished the formation of such vesicles and prevented the release of viral particles from infected cells without significant effects on virus multiplication. Furthermore, the actin-depolymerizing drug, cytochalasin D, inhibited the formation of actin filaments without significantly interfering with formation of vesicular compartments and the release of viruses from treated cells. These results together revealed intracellular vesicular compartments as a mode for viral transport in and release from insect vector cells infected with a plant-infecting reovirus.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/vir.0.2008/002063-0
2008-11-01
2019-11-22
Loading full text...

Full text loading...

/deliver/fulltext/jgv/89/11/2915.html?itemId=/content/journal/jgv/10.1099/vir.0.2008/002063-0&mimeType=html&fmt=ahah

References

  1. Blank, C. A., Anderson, D. A., Beard, M. & Lemon, S. M. ( 2000; ). Infection of polarized cultures of human intestinal epithelial cells with hepatitis A virus: vectorial release of progeny virions through apical cellular membranes. J Virol 74, 6476–6484.[CrossRef]
    [Google Scholar]
  2. Boulanger, D., Smith, T. & Skinner, M. A. ( 2000; ). Morphogenesis and release of fowlpox virus. J Gen Virol 81, 675–687.
    [Google Scholar]
  3. Bugarcic, A. & Taylor, J. A. ( 2006; ). Rotavirus nonstructural glycoprotein NSP4 is secreted from the apical surfaces of polarized epithelial cells. J Virol 80, 12343–12349.[CrossRef]
    [Google Scholar]
  4. Cramer, L. P. & Mitchison, T. J. ( 1995; ). Myosin is involved in postmitotic cell spreading. J Cell Biol 131, 179–189.[CrossRef]
    [Google Scholar]
  5. Durán, J. M., Valderrama, F., Castel, S., Magdalena, J., Tomás, M., Hosoya, H., Renau-Piqueras, J., Malhotra, V. & Egea, G. ( 2003; ). Myosin motors and not actin comets are mediators of the actin-based Golgi-to-endoplasmic reticulum protein transport. Mol Biol Cell 14, 445–459.[CrossRef]
    [Google Scholar]
  6. Fukushi, T., Shikata, E. & Kimura, I. ( 1962; ). Some morphological characters of rice dwarf virus. Virology 18, 192–205.[CrossRef]
    [Google Scholar]
  7. Goddette, D. W. & Frieden, C. ( 1986; ). Actin polymerization. The mechanism of action of cytochalasin D. J Biol Chem 261, 15974–15980.
    [Google Scholar]
  8. Gottlieb, T. A., Ivanov, I. E., Adesnik, M. & Sabatini, D. D. ( 1993; ). Actin microfilaments play a critical role in endocytosis at the apical but not the basolateral surface of polarized epithelial cells. J Cell Biol 120, 695–710.[CrossRef]
    [Google Scholar]
  9. Kimura, I. ( 1986; ). A study of rice dwarf virus in vector cell monolayers by fluorescent antibody focus counting. J Gen Virol 67, 2119–2124.[CrossRef]
    [Google Scholar]
  10. Klausner, R. D., Donaldson, J. G. & Lippincott-Schwartz, J. ( 1992; ). Brefeldin A: insights into control of membrane traffic and organelle structure. J Cell Biol 116, 1071–1080.[CrossRef]
    [Google Scholar]
  11. Kolesnikova, L., Bamberg, S., Berghöfer, B. & Becker, S. ( 2004; ). The matrix protein of Marburg virus is transported to the plasma membrane along cellular membranes: exploiting the retrograde late endosomal pathway. J Virol 78, 2382–2393.[CrossRef]
    [Google Scholar]
  12. Ng, M. L., Tan, S. H., See, E. E., Ooi, E. E. & Ling, A. E. ( 2003; ). Proliferative growth of SARS coronavirus in vero E6 cells. J Gen Virol 84, 3291–3303.[CrossRef]
    [Google Scholar]
  13. Nydegger, S., Foti, M., Derdowski, A., Spearman, P. & Thali, M. ( 2003; ). HIV-1 egress is gated through late endosomal membranes. Traffic 4, 902–910.[CrossRef]
    [Google Scholar]
  14. Omura, T., Hibino, H., Inoue, H. & Tsuchizaki, T. ( 1985; ). Particles of rice gall dwarf virus in thin sections of diseased rice plants and insect vector cells. J Gen Virol 66, 2581–2587.[CrossRef]
    [Google Scholar]
  15. Omura, T., Yan, J., Zhong, B., Wada, M., Zhu, Y., Tomaru, M., Maruyama, W., Kikuchi, A., Watanabe, Y. & other authors ( 1998; ). The P2 protein of rice dwarf phytoreovirus is required for adsorption of the virus to cells of the insect vector. J Virol 72, 9370–9373.
    [Google Scholar]
  16. Pelham, H. R. B. ( 1991; ). Multiple targets for brefeldin A. Cell 67, 449–451.[CrossRef]
    [Google Scholar]
  17. Peterson, A. J. & Nuss, D. L. ( 1985; ). Wound tumor virus polypeptide synthesis in productive noncytopathic infection of cultured insect vector cells. J Virol 56, 620–624.
    [Google Scholar]
  18. Radtke, K., Dohner, K. & Sodeik, B. ( 2006; ). Viral interactions with the cytoskeleton: a hitchhiker's guide to the cell. Cell Microbiol 8, 387–400.[CrossRef]
    [Google Scholar]
  19. Sampath, P. & Pollard, T. D. ( 1991; ). Effects of cytochalasin, phalloidin, and pH on the elongation of actin filaments. Biochemistry 30, 1973–1980.[CrossRef]
    [Google Scholar]
  20. Sasaki, H., Nakamura, M., Ohno, T., Matsuda, Y., Yuda, Y. & Nonomura, Y. ( 1995; ). Myosin-actin interaction plays an important role in human immunodeficiency virus type 1 release from host cells. Proc Natl Acad Sci U S A 92, 2026–2030.[CrossRef]
    [Google Scholar]
  21. Shikata, E. ( 1969; ). Electron microscopic studies on rice viruses. In The Virus Disease of the Rice Plant, pp. 223–240. Baltimore: Johns Hopkins Press.
  22. Shikata, E. & Maramorosch, K. ( 1965; ). Electron microscopic evidence for the systemic invasion of an insect host by a plant pathogenic virus. Virology 27, 461–475.[CrossRef]
    [Google Scholar]
  23. Suikkanen, S., Antila, M., Jaatinen, A., Vihinen-Ranta, M. & Vuento, M. ( 2003; ). Release of canine parvovirus from endocytic vesicles. Virology 316, 267–280.[CrossRef]
    [Google Scholar]
  24. Tartakoff, A. M. ( 1983; ). Perturbation of vesicular traffic with the carboxylic ionophore monensin. Cell 32, 1026–1028.[CrossRef]
    [Google Scholar]
  25. Vidano, C. ( 1970; ). Phases of maize rough dwarf virus multiplication in the vector Laodelphax striatellus (Fallén). Virology 41, 218–232.[CrossRef]
    [Google Scholar]
  26. Wei, T., Kikuchi, A., Moriyasu, Y., Suzuki, N., Shimizu, T., Hagiwara, K., Chen, H., Takahashi, M., Ichiki-Uehara, T. & Omura, T. ( 2006a; ). The spread of rice dwarf virus among cells of its insect vector exploits virus-induced tubular structures. J Virol 80, 8593–8602.[CrossRef]
    [Google Scholar]
  27. Wei, T., Kikuchi, A., Suzuki, N., Shimizu, T., Hagiwara, K., Chen, H. & Omura, T. ( 2006b; ). Pns4 of rice dwarf virus is a phosphoprotein, localized around the viroplasm matrix and forms minitubules. Arch Virol 151, 1701–1712.[CrossRef]
    [Google Scholar]
  28. Wei, T., Shimizu, T., Hagiwara, K., Kikuchi, A., Moriyasu, Y., Suzuki, N., Chen, H. & Omura, T. ( 2006c; ). Pns12 protein of rice dwarf virus is essential for formation of viroplasms and nucleation of viral-assembly complexes. J Gen Virol 87, 429–438.[CrossRef]
    [Google Scholar]
  29. Wei, T., Chen, H., Ichiki-Uehara, T., Hibino, H. & Omura, T. ( 2007; ). Entry of rice dwarf virus into cultured cells of its insect vector involves clathrin-mediated endocytosis. J Virol 81, 7811–7815.[CrossRef]
    [Google Scholar]
  30. Wei, T., Shimizu, T. & Omura, T. ( 2008; ). Endomembranes and myosin mediate assembly into tubules of Pns10 of rice dwarf virus and intercellular spreading of the virus in cultured insect vector cells. Virology 372, 349–356.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/vir.0.2008/002063-0
Loading
/content/journal/jgv/10.1099/vir.0.2008/002063-0
Loading

Data & Media loading...

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error