1887

Abstract

The Borna disease virus (BDV) proteins X and P are translated from a bicistronic viral mRNA. Here, it was shown that the rescue of recombinant BDV from cDNA was enhanced approximately eightfold if reconstitution of the viral polymerase complex was performed with an expression vector encoding X and P rather than P alone. The results provide evidence that appropriate amounts of X reduce the previously reported high sensitivity of the BDV polymerase to imbalances between the viral proteins N and P. These data indicate that X buffers an unfavourable excess of P, thereby stimulating the assembly of functional BDV polymerase complexes.

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2008-06-01
2019-11-20
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References

  1. Briese, T., Schneemann, A., Lewis, A. J., Park, Y. S., Kim, S., Ludwig, H. & Lipkin, W. I. ( 1994; ). Genomic organization of Borna disease virus. Proc Natl Acad Sci U S A 91, 4362–4366.[CrossRef]
    [Google Scholar]
  2. de la Torre, J. C. ( 2002; ). Molecular biology of Borna disease virus and persistence. Front Biosci 7, d569–d579.[CrossRef]
    [Google Scholar]
  3. Martin, A., Staeheli, P. & Schneider, U. ( 2006; ). RNA polymerase II-controlled expression of antigenomic RNA enhances the rescue efficacies of two different members of the Mononegavirales independently of the site of viral genome replication. J Virol 80, 5708–5715.[CrossRef]
    [Google Scholar]
  4. Perez, M., Sanchez, A., Cubitt, B., Rosario, D. & de la Torre, J. C. ( 2003; ). A reverse genetics system for Borna disease virus. J Gen Virol 84, 3099–3104.[CrossRef]
    [Google Scholar]
  5. Poenisch, M., Unterstab, G., Wolff, T., Staeheli, P. & Schneider, U. ( 2004; ). The X protein of Borna disease virus regulates viral polymerase activity through interaction with the P protein. J Gen Virol 85, 1895–1898.[CrossRef]
    [Google Scholar]
  6. Poenisch, M., Wille, S., Ackermann, A., Staeheli, P. & Schneider, U. ( 2007; ). The X protein of Borna disease virus serves essential functions in the viral multiplication cycle. J Virol 81, 7297–7299.[CrossRef]
    [Google Scholar]
  7. Schneemann, A., Schneider, P. A., Kim, S. & Lipkin, W. I. ( 1994; ). Identification of signal sequences that control transcription of Borna disease virus, a nonsegmented, negative-strand RNA virus. J Virol 68, 6514–6522.
    [Google Scholar]
  8. Schneider, U. ( 2005; ). Novel insights into the regulation of the viral polymerase complex of neurotropic Borna disease virus. Virus Res 111, 148–160.[CrossRef]
    [Google Scholar]
  9. Schneider, U., Naegele, M., Staeheli, P. & Schwemmle, M. ( 2003; ). Active Borna disease virus polymerase complex requires a distinct nucleoprotein-to-phosphoprotein ratio but no viral X protein. J Virol 77, 11781–11789.[CrossRef]
    [Google Scholar]
  10. Schneider, U., Schwemmle, M. & Staeheli, P. ( 2005; ). Genome trimming: a unique strategy for replication control employed by Borna disease virus. Proc Natl Acad Sci U S A 102, 3441–3446.[CrossRef]
    [Google Scholar]
  11. Schwardt, M., Mayer, D., Frank, R., Schneider, U., Eickmann, M., Planz, O., Wolff, T. & Schwemmle, M. ( 2005; ). The negative regulator of Borna disease virus polymerase is a non-structural protein. J Gen Virol 86, 3163–3169.[CrossRef]
    [Google Scholar]
  12. Staeheli, P., Sauder, C., Hausmann, J., Ehrensperger, F. & Schwemmle, M. ( 2000; ). Epidemiology of Borna disease virus. J Gen Virol 81, 2123–2135.
    [Google Scholar]
  13. Yanai, H., Hayashi, Y., Watanabe, Y., Ohtaki, N., Kobayashi, T., Nozaki, Y., Ikuta, K. & Tomonaga, K. ( 2006; ). Development of a novel Borna disease virus reverse genetics system using RNA polymerase II promoter and SV40 nuclear import signal. Microbes Infect 8, 1522–1529.[CrossRef]
    [Google Scholar]
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